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OfflineJbrady5555
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Registered: 12/10/19
Posts: 124
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Second attempt at agar w/transfer questions
    #26634193 - 04/28/20 11:26 AM (3 years, 8 months ago)

So my second attempt at agar went much better than the first. Just not as messy and I remembered to get some green food coloring. It’s been about 11 days or so since I made my first again plates and inoculated. I’m starting to get some growth and another member mentioned that I needed to transfer soon so I just made these new plates. Used pastys potato flake agar with 500ml of water 10g agar powder and 40ml of potato flakes and a drop of honey.

When I transfer tomorrow how large of a wedge should I be transferring to each new plate? Am I looking to transfer anything in particular, like thicker pieces of mycelium? Stuff that’s raising off the agar or more flat? Newbie questions I know.  Below are some pics from today’s work. Thanks for any info or constructive criticism.




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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Second attempt at agar w/transfer questions [Re: Jbrady5555]
    #26634264 - 04/28/20 12:19 PM (3 years, 8 months ago)

about grain size more or less. You would want to transfer any mycelial growth that looks to be doing better then the others. I prefer rhizo growth.


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