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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26606513 - 04/16/20 07:21 PM (3 years, 9 months ago) |
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I use pasty plate recipe. I see a consistent growth timeframe of 7-10 days max. My plates are thin and I don’t even let the plate colonize the surface completely. It usually doesn’t start colonizing under the agar until it runs the plate. I’m only leaving a 1/4 inch around w culture from the edge of plate. I don’t know if this helps, I use a big glob of karo syrup by eye. One big glob per 8-10 plates. Now I’m using 1 gram agar powder to this recipe because 2 grams is so solid I can barely cut it with the scalpel.
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FooMan



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Re: EAT LIQUID INOCULATES™ [Re: Bph] 1
#26609793 - 04/18/20 07:50 AM (3 years, 9 months ago) |
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Quote:
Bph said: It's made if "tritan" by eastman plastics. Just says it's durable and won't warp in the dishwasher so I'm thinking its good... The wife will likely kill me if I melt it tho.
The plastic should say dishwasher AND microwave safe. Dishwasher safe does not equal PC safe. That thing will melt in your PC guaranteed. Trying to save a marriage here
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Quick WBS Prep
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tedoro
ToadStool Tender



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Re: EAT LIQUID INOCULATES™ [Re: FooMan]
#26633266 - 04/27/20 11:37 PM (3 years, 9 months ago) |
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You know when you have an idea and you think its smart, but then you realize others have definitely tried it before you....
Has anyone made LI with some water that is slightly sweetened, say with LME? making a poke inoculation super explosive?
Save me some time here... shoot me down.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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poisoned
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633678 - 04/28/20 06:39 AM (3 years, 9 months ago) |
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That's basically a LC then
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: poisoned]
#26633725 - 04/28/20 07:27 AM (3 years, 9 months ago) |
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Quote:
poisoned said: That's basically a LC then
yeah, but is it? With LC, you have all of this uncolonized nutrient, waiting for days to get colonized with a small piece of inoculate.
With this... its a whole colonized plate of mycelia, blended and used immediately.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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A.k.a
Stranger



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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633729 - 04/28/20 07:31 AM (3 years, 9 months ago) |
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I’m not getting it.
A whole plate of blended myc is just the blender li tek right?
Or like the super soft plates that dissolve during shaking.
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LAGM2020     
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poisoned
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26633746 - 04/28/20 07:45 AM (3 years, 9 months ago) |
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But why sweeten then?
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: poisoned] 1
#26633781 - 04/28/20 08:12 AM (3 years, 9 months ago) |
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Quote:
poisoned said: But why sweeten then?
I am trying to chase down why I am having varying leapoff from my LI. And one hypothesis is that the wheat berry bags that had more exploded kernels spread a bit of sugar on the external hulls of the berries...
... so I got to thinking that maybe if I added a bit of sweetening to my LI, I could reproduce the effect without intentionally bursting kernels.
* its not just the blender tek... I'm proposing adding nutes to the blender water
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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A.k.a
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Re: EAT LIQUID INOCULATES™ [Re: tedoro] 1
#26633782 - 04/28/20 08:13 AM (3 years, 9 months ago) |
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Give it a shot man it might speed things up.
One way to find out.
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LAGM2020     
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poisoned
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26633797 - 04/28/20 08:21 AM (3 years, 9 months ago) |
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Make sure to post the results. I'm still figuring out how low should I go with agar. Made it at 0.7% last time, waiting for plates to colonize. It didn't really liquefy at 1% and I had glass shards in that jar. I guess I have some super strong agar here.
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A.k.a
Stranger



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Re: EAT LIQUID INOCULATES™ [Re: poisoned]
#26633804 - 04/28/20 08:24 AM (3 years, 9 months ago) |
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For li plates I used 3-4g agar to 450ml.
I’d make them slightly on the thinner side cuz when you pick a piece up it’ll split in half from its own weight if the agars thicker.
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LAGM2020     
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26633813 - 04/28/20 08:26 AM (3 years, 9 months ago) |
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Quote:
A.k.a said: For li plates I used 3-4g agar to 450ml.
I’d make them slightly on the thinner side cuz when you pick a piece up it’ll split in half from its own weight if the agars thicker.
cool. and 1% nutes?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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A.k.a
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633826 - 04/28/20 08:33 AM (3 years, 9 months ago) |
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Idk usually like 2-4g lme also.
It’s been a minute since I did them but I remember making some with so little agar that instead of setting it made like a syrup.
I keep meaning to go back and make it again cuz it would probably work really well if you dropped a wedge in it and let it colonize.
Even with the soft plates I got a lot of myc growing down into the agar. It looks weird but packs way more myc per same amount of agar than a normal plate.
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LAGM2020     
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26633878 - 04/28/20 09:01 AM (3 years, 9 months ago) |
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I've been doing some sampling of fully colonized 2% agar plates... and I'm starting to think subsurface mycelia colonization is common with most agar recipes.

-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633880 - 04/28/20 09:01 AM (3 years, 9 months ago) |
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what I'm not sure of is the density of that colonization.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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poisoned
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633934 - 04/28/20 09:29 AM (3 years, 9 months ago) |
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Yeah, I always get fluffy growth on the other side of agar puck next day when doing A2G.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: EAT LIQUID INOCULATES™ [Re: poisoned]
#26667179 - 05/12/20 06:15 PM (3 years, 9 months ago) |
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Ted check it out. Tested out a new toy today. It didn’t melt at 15 psi for 30 mins, but it wasn’t closed when I opened the foil. I was thinking it might have warped a little but when I pressed in the cap it was fine. I like the extra protection after each pour. These are nice.

Also a pointer for anyone having issues blending plates. I usually put mine on the liquify setting as it’s the strongest setting on my oster. But I had a fairly thick colonized plate today that wouldn’t break up. So I changed speed to the normal blend setting and it did the trick. So mixing up speed settings on the blender can pulverize the plate of you are having difficulty.
Edited by eatyualive (05/12/20 06:23 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26683352 - 05/20/20 01:18 PM (3 years, 8 months ago) |
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So I had a new record for LI this week. I actually aplplied less inoculant per plate culture. I did roughly 15 quarts to one holy grail plate and the jars colonized in 4 days! I usually do 10. Might be these new pour lids getting a nice even pour.
This was APEU that doesn’t normally colonize this fast but it did. One thing is that a good shake makes the difference. I think I shook nicely and got the speed I always wanted with less inoculant using LI. Generally I’ve only had this speed with cornmeal agar plates. 
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ouuwee
Garden of Weeden


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26683466 - 05/20/20 02:19 PM (3 years, 8 months ago) |
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Can you PC those plastic lids? I'm looking to replace the original lids to plastic but the ball brand plastic lids i've found on amazon states their not used for canning
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: EAT LIQUID INOCULATES™ [Re: ouuwee]
#26683614 - 05/20/20 03:32 PM (3 years, 8 months ago) |
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Yes I’ve done it twice. 30 mins 15 psi. No warping so far and I ordered another pack.
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