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OfflineMusHVAC_HD73
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Reasons spores won’t germinate or grow?
    #26633641 - 04/28/20 05:50 AM (3 years, 8 months ago)

This may be a dumb ? But figured I’d ask the trained pros out their. What are reasons why spores won’t work or grow in brf tek? All help and advice is much appreciated!


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OfflineOne of Us
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Re: Reasons spores won’t germinate or grow? [Re: MusHVAC_HD73]
    #26633665 - 04/28/20 06:17 AM (3 years, 8 months ago)

The spores could be too old, or stored improperly (like frozen for example).

The jars did not cool enough before inoculation.

Or the brf is too wet or dry

It could be that you forgot to shake the syringe to distribute the spores evenly, and you essentially inoculated them with plain water.

Lots of possibilities.


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OfflineLogicaL ChaosM
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Re: Reasons spores won’t germinate or grow? [Re: MusHVAC_HD73]
    #26633682 - 04/28/20 06:43 AM (3 years, 8 months ago)

Mixture too wet.
Mixture too dry.
Too cold (under 59F).
Not Enough Spores.

Those are your main reasons.


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OfflineMusHVAC_HD73
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Re: Reasons spores won’t germinate or grow? [Re: LogicaL Chaos]
    #26633703 - 04/28/20 07:11 AM (3 years, 8 months ago)

What if the jars didn’t cook right could that be one of them? Or will the spores still try to grow no matter what?

And for moisture content if it’s to dry they won’t grow at all? And would it be the same if it’s to wet?


Could it have to do with only wiping the syringe with alcohol wipe instead of flame sterilizing?

Just trying to figure out what is going wrong so I can try and fix it. Thank you I appreciate the info you guys have granted me😁🍄


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OfflineMusHVAC_HD73
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Re: Reasons spores won’t germinate or grow? [Re: One of Us]
    #26633711 - 04/28/20 07:18 AM (3 years, 8 months ago)

Been storing the spores in the refrigerator. And shake before each inoculation. What would you recommend for time to let cool? Before inoculation I usually wait atleast 12 hrs maybe it’s not enough? I was thinking maybe I’m not cooking the jars right? I pressure cook them at 15 psi with tin foil on the loose lids. Only thing is when I pull them out the tin foil looks like there’s like a smoke residue on it. And sometimes has condensation under the tin foil. Maybe to much water in the cooker?


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Invisiblegoatchild
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Re: Reasons spores won’t germinate or grow? [Re: MusHVAC_HD73]
    #26633714 - 04/28/20 07:19 AM (3 years, 8 months ago)

Same thing happened to me recently with 6 jars (PF tek). None of the 6 germinated for 20 days. I figured 20 days was too much so I tossed them out (not the jars itself just the substrate etc).

Also this time I used coffee not from grains but from a coffee machine that uses capsules (I got lazy). Maybe the coffee had some anti-fungal agent/chemical that prevented the fungus from germinating. The rest I did properly: shook the seginges, used the proper amount of water+coffee, SAB, sterile technique etc.

A couple of them also had this smell that reminded me of garbage so maybe those had some bacteria that prevents germination of mushroom spores? The syringes were the same I used for my first PF tek grow and all 12 germinated ok without contamination etc.


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OfflineMusHVAC_HD73
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Re: Reasons spores won’t germinate or grow? [Re: goatchild]
    #26633723 - 04/28/20 07:27 AM (3 years, 8 months ago)

Maybe I’ll try and make a batch with a tad more water and another with a tad less. And try just flame sterilization instead of wiping see if anything changes. I use the 2.1.1 and it’s exact so not sure what’s happening I haven’t had any luck at all this is my third try with nothing.


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OfflineA.k.aM
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Re: Reasons spores won’t germinate or grow? [Re: MusHVAC_HD73] * 1
    #26633800 - 04/28/20 08:22 AM (3 years, 8 months ago)

Is too wet really a thing?

I mean they’re stored in water for syringes and spore lc definitely will grow.


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OfflineOne of Us
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Re: Reasons spores won’t germinate or grow? [Re: A.k.a]
    #26633818 - 04/28/20 08:29 AM (3 years, 8 months ago)

Post some pics if you can. That way we can determine if your jars were too wet/dry or something else.

Also, how long has it been since inoculation?


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