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InvisibleSarz


Registered: 02/07/12
Posts: 15
How do you spawn your tubs?
    #26629472 - 04/26/20 10:47 AM (3 years, 9 months ago)

Hey there,

  Been a long time since I've posted here. Started my first grow from reading here, and have been growing for a few years since. I recently moved into a new house and upgraded my setup to accommodate more. I never had much problem with contamination before, but it seems to be popping up now almost every tub I spawn.

  I've got a small greenhouse setup in my basement for spawning tubs. I usually spray the greenhouse with lysol an hour or so before spawning, as well as wiping the tubs out with alcohol and peroxide. I also added a UVC light that I run for an hour prior to spawning as well inside the greenhouse with my tubs and tools inside. I'm not sure how effective this actually is, but I figured it wouldn't hurt to try. I typically spawn in just my boxers so I don't have clothes on that might trap any dust or mold. I shower beforehand, and usually clean my hands and arms with alcohol before I enter the greenhouse. I wear gloves that I also clean with alcohol before I start working, and I wipe everything that I will handle with alcohol (jars, bucket, tubs). I also give the tubs another quick wipe down with iso alcohol. I usually wear a respirator as well due to the alcohol fumes.

  I then spawn my tubs. 1 brick coco coir, 3 quarts verm, handful of gypsum, 4 quarts of spawn. Seal em up and let them go for 14 days.

They usually colonize fully, and after I've opened them and polyfilled they tend to contam within a few days. I am wondering if my this is due to my spawning practices, or if there is something in the environment where they are fruiting that is getting them after they've been opened.

I also want to know how anyone else spawns their tubs. What methods do you use? How do you stay sterile when spawning tubs?

I've gotten my jar colonization to 100% with my homemade still air box, and I've been ecstatic about that. But these tubs contaminating 3 days after they've been opened is really discouraging.

I had a lot of luck last summer with my tubs. I did have a few that contaminated though, and I am also wondering if it has just fucked my entire basement from ever being a viable place for growing.


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OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house Flag
Last seen: 22 hours, 50 minutes
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Re: How do you spawn your tubs? [Re: Sarz] * 1
    #26629516 - 04/26/20 11:06 AM (3 years, 9 months ago)

Contamination is from your spawn. Sterile doesn't matter when your spawning. Once your grains are colonized your good. I'll spawn in my living room smoking a cigarette and scratching my balls. IF your spawn is good you wont have green, at least until flush 2 or so. Some people get 5.


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Crack's Tek's and Shit



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InvisibleRoger Clemency
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Registered: 03/23/20
Posts: 2,005
Re: How do you spawn your tubs? [Re: Crackatoa] * 1
    #26629563 - 04/26/20 11:19 AM (3 years, 9 months ago)

What kind of contamination are you getting? Trich? Bright white that turns green..

Like crack says, scratch balls + spawn tub = 🍄 ...pretty much. As long as you had clean grain spawn to start with. How are you inoculating your grains? How are you ‘sealing up’ your tubs after spawning?


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Sour grapes, sweet revenge
Heaven starts right where hell ends


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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
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Loc: Milky way
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Re: How do you spawn your tubs? [Re: Roger Clemency] * 1
    #26629571 - 04/26/20 11:22 AM (3 years, 9 months ago)

Sealing them up for a 14 long ass day colonization is a prime environment for contaminants


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OfflineSockadin
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Registered: 01/03/10
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Re: How do you spawn your tubs? [Re: Sarz] * 1
    #26629593 - 04/26/20 11:27 AM (3 years, 9 months ago)

Check your grain jars. You will find your contamination there most likely.


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,755
Re: How do you spawn your tubs? [Re: Roger Clemency]
    #26629653 - 04/26/20 11:51 AM (3 years, 9 months ago)

Quote:

The Abhorrent Chef said:
How are you inoculating your grains?




:whathesaid:


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InvisibleSarz


Registered: 02/07/12
Posts: 15
Re: How do you spawn your tubs? [Re: LadysKnight]
    #26632011 - 04/27/20 11:40 AM (3 years, 9 months ago)

For Inoculation I use a MS  syringe to liquid culture, then LC to grain. I should note I did not start working with LC until I moved into this house.

I'm sure it's entirely possible that my LC is contaminated. Makes me really want to switch it up and start working with agar.

I use a SAB with alcohol lamp and jars with resealable injection ports. ~3cc of LC per jar.

Let them colonize usually 3-4 weeks, and then spawn.

I recently switched to a new style tub. Those sterilites with the gasket on the inside of the lid to seal and latches on the side. The lids stayed on nicely, and were easy to move around.

I also recently switched to a differently kind of micropore tape because the one i was using wasn't sticking very well. After doing this I noticed early fruiting, and I felt like it was due to more FAE. I looked closer at the tape and it was perforated in the middle, so I decided to just use duct tape this time, so they were extra sealed. I imagine probably a fatal error.

Let them sit for 14 days. =/  Only 2 showed contamination before I opened them. The rest flourished as soon as they were opened.

Also: Contam is trich

The feedback I have received so far has been extremely valuable. Thank you all.


Edited by Sarz (04/27/20 02:09 PM)


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OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house Flag
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Re: How do you spawn your tubs? [Re: Sarz] * 1
    #26632039 - 04/27/20 11:55 AM (3 years, 9 months ago)

Spore syringe to LCs or straight to grain isn't advised. You should get into agar for allot more success. There's a link in my signature that's a no pour agar with very cheap containers of you want to get started cheaply.


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Crack's Tek's and Shit



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OfflineGrowtech
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Registered: 11/09/19
Posts: 165
Last seen: 2 years, 5 months
Re: How do you spawn your tubs? [Re: Sarz] * 1
    #26632046 - 04/27/20 12:03 PM (3 years, 9 months ago)

Quote:

Sarz said:
For Inoculation I use a MS spore syringe to liquid culture, then LC to grain. I should note I did not start working with LC until I moved into this house.

I'm sure it's entirely possible that my LC is contaminated. Makes me really want to switch it up and start working with agar.



In all likelihood, your first step there is the issue.

Assume all spore syringes are contaminated.  Even if you don't see contamination when you use them, still assume they are contaminated.  I'm not sure if I've ever run across a truly contamination-free spore syringe (it's just a matter of how quickly you can tell they are contaminated).

That's not to say you can't have success with contamination (people do multi-spore syringe straight to grain and get decent results every day), however you will *always* have better results if you start with something clean (mycelium spends less time/energy fighting contaminants, and more time/energy making mushrooms).


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Mycolo.gyGenetics & yield tracking system


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OfflineGrungeman17
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Registered: 05/06/09
Posts: 1,438
Loc: usa Flag
Last seen: 4 hours, 29 minutes
Re: How do you spawn your tubs? [Re: Growtech]
    #26632059 - 04/27/20 12:09 PM (3 years, 9 months ago)

Im about to do three all in trash bags, set it in there and cut above the sub level below the bottom big holes.


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