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Offlinerickysavage
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I have done PF Tek and want to go bigger. I don't know where to start.
    #26630539 - 04/26/20 06:33 PM (3 years, 9 months ago)

Actually, I am on my second trial of PF Tek. I have 10 jars that have been sterilized and inoculated. I am waiting for colonization. Additionally, I have 5 spore syringes on the way, arriving Wednesday and I wanted to try a bulk technique.

I have done a ton of reading here, other places on the internet, watching youtube videos and I am getting the hang of the process in general.

I am no expert and wanted to follow a technique that is proven. The thing is, I don't know where to start. Even with "bod" I find his threads overwhelming with information without a clear path. What is a bulk technique that I can start with, that provides a clear path?

Still a beginner trying to learn.


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Offlinerickysavage
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: rickysavage]
    #26630584 - 04/26/20 06:59 PM (3 years, 9 months ago)

If I am following BOD's Easy AF unmodified monotub, am I right that these would be the best step? I am using a spore syringe, not agar.
  • make grain spawn with oats
  • inoculate grain spawn with spore syringes, allow colonization
  • make substrate
  • mix substrate and grain spawn in monotub
  • change to growing conditions


I am finding a hard time finding the ratio of grain spawn to substrate


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InvisibleRoger Clemency
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: rickysavage]
    #26630593 - 04/26/20 07:02 PM (3 years, 9 months ago)

Between 1:1 and 1:4 spawn to bulk sub.

Those are the steps basically, you’ll not know what your putting in those grains until it germinates so you can end up wasting a lot of time and grain. Agar is super easy, just another process and basically necessary for growing in bulk.

There is a flow chart of the process as the first link on the “everything you need to know” page


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Offlinerickysavage
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: Roger Clemency]
    #26630601 - 04/26/20 07:05 PM (3 years, 9 months ago)

Okay. So as long as ratio is between 1:1 and 1:4, that is okay?

After making grain spawn, I can inoculate with syringe, correct?


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InvisibleRoger Clemency
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: rickysavage]
    #26630616 - 04/26/20 07:14 PM (3 years, 9 months ago)

Yup.

You can if you must


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OfflineEnkidu
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: Roger Clemency]
    #26630634 - 04/26/20 07:24 PM (3 years, 9 months ago)

Some people it works some dont
Syringe to grain was hard for me when starting

Agar is easy bro

Mix 3 ingredients, load in pc, cook, let cool, pour plates in SAB, let cool, transfer sample

Easy as pie and much better for going to bulk

The thing is there are a lot of different teks for the same thing so a lot of personal preference

You got the general idea

Personally i do wbs
Spawn to coir and verm

7 or 8 qt of spawn to a brick of coir and verm is how i do it

Theres a lot of leeway with spawn sub ratio


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Offlinerickysavage
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: Enkidu]
    #26630673 - 04/26/20 07:56 PM (3 years, 9 months ago)

Okay, I will look into agar. The steps just look so overwhelming especially when I am already trying to learn monotub method from PF TEK. Its another barrier.

Can you make agar from spore syringes? Or Where does the "important" ingredient come from?


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Offlinerickysavage
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: Enkidu]
    #26630678 - 04/26/20 08:01 PM (3 years, 9 months ago)

If I am using agar, my updated steps go like this, correct?

  • make grain spawn with oats
  • add agar to grain spawn quart jar, allow colonization
  • make substrate
  • mix substrate and grain spawn in monotub, 1:1 to 1:4 grain to sub ratio
  • change to growing conditions


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OfflineHamHead
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: rickysavage]
    #26630693 - 04/26/20 08:10 PM (3 years, 9 months ago)

First step would be to germinate spores on agar, then clean up a culture to drop to grains. Germination takes anywhere from 3 days to 4 weeks, typically about a week or so, IME.

Plenty of time to keep reading. And reading. And reading.


--------------------
The Italian researchers’ findings, published by the INT’s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February.

https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF

This online first version has been peer-reviewed, accepted and edited,  but not formatted and finalized with corrections from authors and proofreaders

https://www.icandecide.org/


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InvisibleFooMan
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: rickysavage] * 1
    #26630759 - 04/26/20 08:52 PM (3 years, 9 months ago)

You can use colonized cakes to inoculate grains. Check THIS out. You can inoculate several quarts of grains with each cake. I haven't found a faster way to colonize grain.


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OfflineLogicaL ChaosM
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: rickysavage]
    #26631002 - 04/26/20 11:09 PM (3 years, 9 months ago)

I would recommend PF Tek to bulk, aka Crumble and Case. Basically mixing colonized PF cakes with uncolonized grains. Shoebox tek is probably the best way for start bulk using PF cakes as the starting material. Hope this helps.


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Offlinepoisoned
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: LogicaL Chaos] * 1
    #26631246 - 04/27/20 03:01 AM (3 years, 9 months ago)

Going BRF to bulk is viable IMO, but don't involve any grains in process. Just mix the cakes with bulk substrate like coir.

I'd also recommend you to start with shoeboxes instead of monotubs.


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How I do glass dishes


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OfflineLogicaL ChaosM
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: poisoned]
    #26631253 - 04/27/20 03:08 AM (3 years, 9 months ago)

Whats the main disadvantage of adding colonized PF cake pieces to grain? Is there more of a risk of contamination?


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Offlinepoisoned
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: LogicaL Chaos]
    #26631259 - 04/27/20 03:14 AM (3 years, 9 months ago)

If you can do grains, why wouldn't you start with grains? They are way faster than cakes, they will crumble if you bang the jars against your hand or a tire and they don't have any dirty stuff inside the jar.

PF tek jars have contaminants inside on the dry verm layer and probably the glass too.


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OfflineLogicaL ChaosM
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: poisoned]
    #26631266 - 04/27/20 03:18 AM (3 years, 9 months ago)

You can start with grains, but you would have to learn agar too. Maybe OP is in a hurry :wink:

I would recommend this for someone who was transitioning from PF Tek to Grains:  https://www.shroomery.org/forums/showflat.php/Number/20410651#20410651


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Offlinepoisoned
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: LogicaL Chaos]
    #26631273 - 04/27/20 03:24 AM (3 years, 9 months ago)

MS->Grain seems like a more valid approach than this. Eat knows his shit tho, so IDK.


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OfflineLogicaL ChaosM
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: poisoned]
    #26631277 - 04/27/20 03:30 AM (3 years, 9 months ago)

To my understanding, spore syringe is too dirty for MS to syringe. Although straight off a spore print *might* work but not sure :shrug:

I feel like PF Tek to Grain is the safer pathway. Eat definitely knows his shroomz. Top of the Class :mushroom2:


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OfflineInnerWisdom
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: Enkidu]
    #26631278 - 04/27/20 03:31 AM (3 years, 9 months ago)

By qt/quarts do people always mean the actual volume of 0.94liters or whatever the exact number was? Cos that is a lot of spawn


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OfflineLogicaL ChaosM
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: InnerWisdom]
    #26631280 - 04/27/20 03:33 AM (3 years, 9 months ago)

It means you can fit a quart of grain inside a quart sized jar. Bulk is all about huge amounts of spawn :lol:

Also should be noted to not fill a quart jar completely full. 75% full of grain is best so you can shake the jar if needed.


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OfflineInnerWisdom
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Re: I have done PF Tek and want to go bigger. I don't know where to start. [Re: LogicaL Chaos] * 1
    #26631285 - 04/27/20 03:41 AM (3 years, 9 months ago)

I actually might use my colonizing 3 brf jars to inoculate grains like in the tek you linked. Then I only need a PC but no agar so I could get more shrooms faster :crazy2::lol:


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