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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
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PF Cake Slurry to Grain Bags?
#26631169 - 04/27/20 01:32 AM (3 years, 8 months ago) |
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Is it bad practice to make a slurry from a colonized PF cake and then add it to a bag of grain? Is this pretty safe/reasonable or is it prone to infection?
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poisoned
untitled



Registered: 04/17/13
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Sounds like a bad idea. How would you even do the slurry and transfer in a clean matter? Remember, that top layer of verm is dirty.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
Last seen: 1 hour, 31 minutes
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Re: PF Cake Slurry to Grain Bags? [Re: poisoned]
#26631233 - 04/27/20 02:51 AM (3 years, 8 months ago) |
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In a really large SAB?
I would clean the cake first with clean water, top verm and all, then blend then add to a bag of grain.
But maybe crumbling a freshly-cleaned dunked cake may be better than a slurry....
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Ovoidhunter
Buttery Crescent



Registered: 09/17/16
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I did it once and it worked but it went bacterial. Probably because it was an MS cake tho.
I didn't put it to grains tho I inoculated more cakes. Kinda like an LC or something dunno what you would call it. It didn't work out to well.
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
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Re: PF Cake Slurry to Grain Bags? [Re: Ovoidhunter] 1
#26631241 - 04/27/20 02:57 AM (3 years, 8 months ago) |
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Sorry, but it sounds like a shit ton of contamination vectors to me. Why would you even do that?
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Ovoidhunter
Buttery Crescent



Registered: 09/17/16
Posts: 2,016
Last seen: 2 years, 8 months
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Re: PF Cake Slurry to Grain Bags? [Re: poisoned] 1
#26631245 - 04/27/20 03:00 AM (3 years, 8 months ago) |
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Quote:
Why would you even do that?
Because trying new stuff is fun. Its okay to deviate from the tek sometimes.
The tek does not control your being.
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poisoned
untitled



Registered: 04/17/13
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Loc: Yurop
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Re: PF Cake Slurry to Grain Bags? [Re: Ovoidhunter]
#26631252 - 04/27/20 03:05 AM (3 years, 8 months ago) |
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I definitely do deviate from teks quite often, but this sounds so counterproductive. You're not gaining either time, simplicity or cleanliness with this approach.
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Ovoidhunter
Buttery Crescent



Registered: 09/17/16
Posts: 2,016
Last seen: 2 years, 8 months
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Re: PF Cake Slurry to Grain Bags? [Re: poisoned]
#26631255 - 04/27/20 03:09 AM (3 years, 8 months ago) |
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No doubt about that. When I tried it I ground up cakes in a ziplock bag with water squished it up drained the water out sucked it up in a syringe and used it as inoculant. It colonized but got contamed with bacteria. Probably fruited it in a mason jar.
I don't do that stuff anymore tho. Cakes are now in my past. Thank god.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
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Re: PF Cake Slurry to Grain Bags? [Re: Ovoidhunter]
#26631256 - 04/27/20 03:11 AM (3 years, 8 months ago) |
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Found what I needed: https://www.shroomery.org/forums/showflat.php/Number/20410651#20410651
Posting for Reference. Special thanks to eatyoualive
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Yeah Eats has amax Zing success with Slurry cakes.
The most important thing is to follow the TEK to a T. Open jar in SAB make sure your using sterile TEK. 1 Jar can get like 3-4 bags colonized in 1/4 of the time for AGAR wedges.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
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Re: PF Cake Slurry to Grain Bags? [Re: Sockadin]
#26631471 - 04/27/20 06:07 AM (3 years, 8 months ago) |
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Definitely. I just upgraded my SAB so it should be good for more advanced work such as this.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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I want to mod my jelly jars with.the oyster lids and install ships in them.
They you can make your cake. Inoculate it and blend at 100% with out ever removing the blender lid until your in the SAB.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
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Re: PF Cake Slurry to Grain Bags? [Re: Sockadin]
#26631481 - 04/27/20 06:13 AM (3 years, 8 months ago) |
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I just saw that tek! But I forgot what it was called....
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
Last seen: 1 hour, 31 minutes
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Roadkill makes a pretty cool comment in this old thread: https://www.shroomery.org/forums/showflat.php/Number/812249
Looks like a "dry slurry" is best.
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