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Offlinerobertplant
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Agar - is ANY of this mycelium?
    #26630257 - 04/26/20 04:22 PM (3 years, 8 months ago)

So far, ALL the agar plates I've tried have been contaminated.  I've tried spore syringes and recently some tissue cuts from inside fruit stems (first photo).  While I know they are contaminated I'm just surprised that there seems to be zero mycelium on any of my plates.  So, I dug a little deeper.  Seems the pre-poured plates I bought are tryptic soy agar with Cycloheximide which is "for use as a general growth medium for the isolation and cultivation of microorganisms while inhibiting Saprophytic fungal organisms." 

Tossed them all and took new tissue cuts today on new medium.  Keep your fingers crossed for me!









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InvisibleTheStallionMang
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Registered: 10/18/17
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Re: Agar - is ANY of this mycelium? [Re: robertplant]
    #26630276 - 04/26/20 04:33 PM (3 years, 8 months ago)

I'm pretty sure with anti-fungal agar you're just going to be growing mold and bacteria. I don't see anything that looks like myc

Try checking your local Asian grocery market for agar-agar and you probably want to add some nutes.

Check out the "no pour agar dishes" link in my signature if you need some quick and cheap petri dishes


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