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rezasmith
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Registered: 01/27/20
Posts: 92
Last seen: 3 years, 5 months
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Question about an old AA tek
#26629840 - 04/26/20 01:05 PM (3 years, 9 months ago) |
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so i came across this tek and i think it's pretty useful for me since All my contams were bacteria so far i was wondering if there is an updated version of this or this simply do the job? https://www.shroomery.org/forums/showflat.php/Number/17356799 thank you
Edit : is there any other kind of antibiotics that can be used instead of Furan-2?
Edited by rezasmith (04/26/20 01:07 PM)
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redhandmat
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Registered: 05/09/19
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Re: Question about an old AA tek [Re: rezasmith]
#26629870 - 04/26/20 01:16 PM (3 years, 9 months ago) |
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I would personally say that you should work harder on your sterile tek first and foremost. Like really down to the nitty gritty cleanliness.
Once you got that down you can make use of hotpours to sandwich your agar culture between two layers of agar and the healthy mycelium should outrun the bacteria to the top of the plate after some time. Then you quickly transfer the growths to new plates and see which one will turn out to be mycelium. I have successfully done this as an absolute nooob for the first plate I manages to get some myc I had a ton of bacteria. And when its all said and done, the whole point of agar is to transfer away from contams several times till we get a clean culture.
I was also recommended to add black tea to my agar for those transfers as it have shown to have antibacterial properties:
Quote:
LadysKnight said: You don't need antibiotics. Black tea agar works great to freeze bacteria in place. But I think you can clean these up with just a couple transfers.
https://www.shroomery.org/forums/showflat.php/Number/23860431/fpart/1/vc/1
But if however you really want to do antibiotics then Ihave a mushroom growth book (By Rusell Stephen) and it says this:
"Stamets recommends 100 mg per liter based on Gentamycin that’s 60 to 80 percent pure, so you may need to adjust your formula based on the purity of the product you obtain. Use less (closer to 50 mg) of a product that has a higher purity"
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rezasmith
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Registered: 01/27/20
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Last seen: 3 years, 5 months
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Re: Question about an old AA tek [Re: redhandmat]
#26629940 - 04/26/20 01:41 PM (3 years, 9 months ago) |
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do you think it's possible to use tetracycline? i cant find the other ones easily at all so thats why im asking
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redhandmat
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Registered: 05/09/19
Posts: 463
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Re: Question about an old AA tek [Re: rezasmith]
#26629993 - 04/26/20 02:02 PM (3 years, 9 months ago) |
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SO my search on forums found this:
Pastywhite has apparently used it so its possible to use it, and from what he says you kind of understand that tetra should be added to agar AFTER the agar plates have cooled. Not sure about BEFORE sterilization.
Quote:
Pastywhyte said: Back on topic I like the idea of the bath to bring down to temp. I am a cheap fucker and prefer to by tetracycline from the pet store than gentamiacin. The bath would speed up the wait for the agar to cool enough to add the tetra.
Trusted cultivator said this:
Quote:
Diploid said: if you are hydrating spores in sterile water, and you add just a tiny bit of the Tetracycline - enough just to tinge your spore-water light yellow - you will NOT develop bacterial contamination.
Antibiotics shouldn't be used preemptively.
Antibiotics treat the symptoms of contamination, not the problem. If you have a contamination problem, perhaps because of sloppy sterile technique or a dirty grow room, you should improve your technique or clean your grow room.
Trying to fix a contamination problem with antibiotics will select for antibiotic resistance in the vector. When that happens, antibiotics will no longer work and you'll have an even bigger problem then when you started.
Use antibiotics very sparingly and only when you're sure all other measures to block contamination are optimized so you don't end up with super-bacteria roaming your lab.
Just my 2 cents.
This guys is not a trusted cultivator, and was actually asking same question as you , later he wrote this in the thread:
Quote:
District said: Bumping this.
Most research recommend a 'pinch' of tetracycline. I want to share my experience with concentration.
I usually run about 250ml agar solution. With a 'pinch' I found that bacteria still blooms. So, I would say, 1 tsp per 250ml is a safe amount based on my experience.
Edited by redhandmat (04/26/20 02:04 PM)
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