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Offlinekeegan.wrathmall
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Registered: 01/19/20
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How to renew vigour with slow grain colonisation? * 1
    #26629797 - 04/26/20 12:46 PM (3 years, 8 months ago)

I have some Oyster grain that I’ve transferred a few times now (always onto same grain). They have almost stopped colonising at this point and the mycelium looks very tomentose and fluffy. What is the next step now? Am I supposed to take spores from the fruit and go that route or should you take a tissue sample from the fruit and onto agar? I’m guessing cloning with agar is the same as G2G and will not solve this problem... in addition, has it been proven that having your transfers on the same grain negatively impacts the general health of your mycelium?


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OfflineForresterM
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Re: How to renew vigour with slow grain colonisation? [Re: keegan.wrathmall]
    #26629904 - 04/26/20 01:29 PM (3 years, 8 months ago)

Are you sure it's senescence causing this and not a contamination in the grain? 

You could try running it across some different types of agar, with different nutrients in it.  I'm not sure if it's been proven that always using the same grain can hurt the health of your culture, but I have heard of people running across different agar recipes to fix it.

I'm not an expert in this field though, so maybe others can give a better answer.  I'd just get a new culture...


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinekeegan.wrathmall
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Re: How to renew vigour with slow grain colonisation? [Re: Forrester]
    #26637898 - 04/29/20 11:28 PM (3 years, 8 months ago)

Quote:

Forrester said:
Are you sure it's senescence causing this and not a contamination in the grain? 

You could try running it across some different types of agar, with different nutrients in it.  I'm not sure if it's been proven that always using the same grain can hurt the health of your culture, but I have heard of people running across different agar recipes to fix it.

I'm not an expert in this field though, so maybe others can give a better answer.  I'd just get a new culture...




It’s not necessarily patchy colonisation which would make me think it’s bacterial, could be some sort of mould but I would expect it to explode in there if it were. Maybe my moisture content is off.

I will probably just go to the super market and clone another fruiting body but I was wondering how the guys do it with strains that are not accessible on the web or in the supermarket, spores is the only way I’m guessing.


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Offlinebogdancev
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Re: How to renew vigour with slow grain colonisation? [Re: keegan.wrathmall]
    #26638785 - 04/30/20 11:15 AM (3 years, 8 months ago)

Spores will give you unpredictable characteristics.
You rather clone from known nice fruiting body or buy mycelium/spawn from cultivators.


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OfflineSHROOMSISAY01M
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Re: How to renew vigour with slow grain colonisation? *DELETED* [Re: keegan.wrathmall]
    #26639156 - 04/30/20 02:19 PM (3 years, 8 months ago)

Post deleted by SHROOMSISAY01

Reason for deletion: I thought he was talking about sub.


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Offlinekeegan.wrathmall
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Registered: 01/19/20
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Re: How to renew vigour with slow grain colonisation? [Re: SHROOMSISAY01]
    #26640400 - 05/01/20 02:59 AM (3 years, 8 months ago)



This is what some of the stalled jars look like. I’ve moved them towards my radiator, could be a bit cold at the moment but the grain seems pretty well hydrated... I have never done any sectoring as I’m just a new hobby grower so maybe I’ve got some poor genetics to couple with any other negatives that are going on.


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OfflineForresterM
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Re: How to renew vigour with slow grain colonisation? [Re: keegan.wrathmall]
    #26643436 - 05/02/20 10:54 AM (3 years, 8 months ago)

Hmm... that is strange.  I don't see any obvious contamination, maybe you're right about the culture :confused:


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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