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Offlinetomcards
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #26739342 - 06/12/20 11:13 AM (3 years, 9 months ago)

Ah, it's good to know that a little carbon won't hurt.  In fact, it may be beneficial.  Anecdotal reports indicate that, to some degree, charcoal protects the agar/myc from contams. 

I love the photos of your black agar and the contrast it provides. 

Thanks again!

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Invisibleeatyualive
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Re: D3's Perfect transfers and agar TEK. [Re: tomcards] * 1
    #26739628 - 06/12/20 01:30 PM (3 years, 9 months ago)



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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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OfflineSockadin
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Re: D3's Perfect transfers and agar TEK. [Re: eatyualive]
    #26746632 - 06/15/20 01:37 PM (3 years, 9 months ago)

So I started using like the 5/16 punch opting for a smaller sample size. I started out with the 3/8. I am noticing a lot more organization in the growth and less sectoring.

Will post pictures in a day or two of some Nats!

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InvisibleD3_Myc
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Registered: 05/06/18
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Re: D3's Perfect transfers and agar TEK. [Re: Sockadin]
    #26747374 - 06/15/20 07:14 PM (3 years, 9 months ago)

Think I started with the 5/16 and ended up down to a quarter.




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Invisiblesandman420
Saint PP
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Registered: 06/17/04
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #26762432 - 06/21/20 07:58 AM (3 years, 9 months ago)

This is great stuff I will be trying this soon. What fantastic pictures too. You can really see the difference in clarity in good old fashioned PS petri dishes versus PP dishes that eats is using. It's night and day truly. Disposables are king, shame about the waste issue.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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InvisibleLadysKnight
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Registered: 10/09/15
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Re: D3's Perfect transfers and agar TEK. [Re: sandman420]
    #26762454 - 06/21/20 08:22 AM (3 years, 9 months ago)

Quote:

sandman420 said:
This is great stuff I will be trying this soon. What fantastic pictures too. You can really see the difference in clarity in good old fashioned PS petri dishes versus PP dishes that eats is using. It's night and day truly. Disposables are king, shame about the waste issue.




Glass is an option.

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InvisiblebodhisattaMDiscordReddit
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Re: D3's Perfect transfers and agar TEK. [Re: sandman420] * 2
    #26762468 - 06/21/20 08:33 AM (3 years, 9 months ago)

Quote:

sandman420 said:
This is great stuff I will be trying this soon. What fantastic pictures too. You can really see the difference in clarity in good old fashioned PS petri dishes versus PP dishes that eats is using. It's night and day truly. Disposables are king, shame about the waste issue.



I think of waste from a use standpoint. The dishes lead to plenty good. But something like a water bottle or straw are pretty useless. Most people willingly waste plenty of plastic and water all day but throw a fit at it in the mycology perspective. I don't get it.

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Invisiblesandman420
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Re: D3's Perfect transfers and agar TEK. [Re: bodhisatta]
    #26762481 - 06/21/20 08:39 AM (3 years, 9 months ago)

no doubt I think thats a fair way to see it. I have a case of glass dishes in storage and use my trusty disposables every day. Because they are just better.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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InvisibleHobbit GDF
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Re: D3's Perfect transfers and agar TEK. [Re: sandman420] * 1
    #26763082 - 06/21/20 01:23 PM (3 years, 9 months ago)

I love the punch. It makes things faster if you got your sterile tek down.

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OfflineIrishmule169
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Re: D3's Perfect transfers and agar TEK. [Re: Hobbit GDF] * 1
    #26777432 - 06/23/20 05:48 PM (3 years, 8 months ago)



😬


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Invisiblesandman420
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Re: D3's Perfect transfers and agar TEK. [Re: Irishmule169]
    #26777553 - 06/23/20 06:52 PM (3 years, 8 months ago)

Is that some kind of tape Hobbit? Nice pic.

I ordered a piece of 1/4" stainless seamless tubing that should be here soon. It should make a good punch with less thermal mass. Stoked.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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InvisibleHobbit GDF
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Re: D3's Perfect transfers and agar TEK. [Re: sandman420] * 1
    #26777577 - 06/23/20 07:05 PM (3 years, 8 months ago)

Yeah its graphting tape for plants. Just like Suran wrap. That pic is before I knew to stretch it. Now it doesn't look so sloppy.

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OfflineSoupyGeorge
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Registered: 05/25/20
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc] * 1
    #26780685 - 06/24/20 10:59 PM (3 years, 8 months ago)

Just here for the tek, a pic of some black looking agar brought me here. The contrast of the mycelium to the agar hooked me. Thanks my dude.


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Mellow heads will prevail.

You look like everyone else because the T.V. tells you to.
I look like everyone else so that i don't stand out to the man.
We are not the same.

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Offlinelabbar
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Re: D3's Perfect transfers and agar TEK. [Re: SoupyGeorge]
    #26783706 - 06/26/20 05:26 AM (3 years, 8 months ago)

Thank You D3monic for amazing tech :thumbup:



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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: D3's Perfect transfers and agar TEK. [Re: Hobbit GDF]
    #26783874 - 06/26/20 07:39 AM (3 years, 8 months ago)

Quote:

Hobbit GDF said:
Yeah its graphting tape for plants. Just like Suran wrap. That pic is before I knew to stretch it. Now it doesn't look so sloppy.




Responded to this a few days ago but I must not of hit the confirm button. (I’m always doing this 😔)

I’ve been using this grafting tape with good success. Just got to be careful not to stretch TOO tight or it splits along the plate seams.

Parafilm Nursery Grafting Tapes... https://www.amazon.com/dp/B00G3Q5O7O?ref=ppx_pop_mob_ap_share

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OfflineVernsMB
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Registered: 05/13/20
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #26784573 - 06/26/20 12:16 PM (3 years, 8 months ago)

Excellent Agar Tek!  I need to find that food coloring!  what agar recipes do you use besides the MEA?  Do you use any other supplements like Yeast Extract, Yeast Nutrient, peptone or DAP(DiAmmonium Phosphate)?

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InvisibleD3_Myc
Weeb Trash
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Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: D3's Perfect transfers and agar TEK. [Re: VernsMB]
    #26784790 - 06/26/20 01:31 PM (3 years, 8 months ago)

No not yet, tried yeast and my plates went haywire. Took a few xfers to recover.

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InvisiblecArcace-x
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Registered: 10/17/13
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc] * 1
    #26786918 - 06/27/20 10:06 AM (3 years, 8 months ago)

:hellyeah:
Hell yeah!
Bookmarked


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InvisibleD3_Myc
Weeb Trash
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Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: D3's Perfect transfers and agar TEK. [Re: labbar]
    #26786947 - 06/27/20 10:15 AM (3 years, 8 months ago)

Quote:

labbar said:
Thank You D3monic for amazing tech :thumbup:






Not sure how I feel about that myc. Post back once it's grown in some more. If it was my plate I'd be extremely suspect and possibly toss it in the scrap pile. Then again i'm notorious for tossing probably perfectly good plates.

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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,852
Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #26786963 - 06/27/20 10:23 AM (3 years, 8 months ago)

Quote:

D3monic said:
Quote:

labbar said:
Thank You D3monic for amazing tech :thumbup:






Not sure how I feel about that myc. Post back once it's grown in some more. If it was my plate I'd be extremely suspect and possibly toss it in the scrap pile. Then again i'm notorious for tossing probably perfectly good plates.




Agreed, looks like mold. Glad you said it first.

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