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Invisiblec10h12n2o
serial dilutor
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Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: D3's Perfect transfers and agar TEK. [Re: Sockadin]
    #27253309 - 03/15/21 02:47 AM (2 years, 10 months ago)

How are you liking the charcoal plates?

I love getting such a good view of the agar surface that i dont feel like a need to backlight them to do close inspection


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Offlinehongito33
Lord of the detritivores


Registered: 10/22/18
Posts: 144
Loc: in your head Flag
Last seen: 1 year, 7 months
Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27253315 - 03/15/21 02:54 AM (2 years, 10 months ago)

D3monic,

You sir are worthy of being call a true evil satan loving genius!!! Not only did your write up is at a level that ebbs and flows so the average block can follow along.  Your video just out right incited in the desire me to be a mad agar disciple. I think it was the wicket rock, fuck that was wood ROCK!!!!!!!!

Thank you again for a wicked write up!!


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Invisiblec10h12n2o
serial dilutor
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Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: D3's Perfect transfers and agar TEK. [Re: hongito33]
    #27253318 - 03/15/21 02:58 AM (2 years, 10 months ago)

He killed it :smile:

I love the ghost track in the background, that album was amazing


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: D3's Perfect transfers and agar TEK. [Re: c10h12n2o]
    #27253395 - 03/15/21 05:19 AM (2 years, 10 months ago)

Thanks guys *blushing

One habit I have worth note is not mixing, shaking or really swirling the media bottles once they are done PC. I also leave the last 1/4-1/8th inch in the bottle so that if there is any sediment it stays settled out in the bottom.


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc] * 1
    #27254577 - 03/15/21 07:11 PM (2 years, 10 months ago)

I have to say that between your tek and C10's you guys have really upped my agar game. I'm getting nice clean plates with no sediment. The nutrient profile looks good and the color compliments my Black Sunflower seeds nicely.


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OfflineGoldenBoy
Stranger
Registered: 10/19/20
Posts: 17
Last seen: 1 year, 4 months
Re: D3's Perfect transfers and agar TEK. [Re: Sockadin]
    #27256436 - 03/16/21 11:05 PM (2 years, 10 months ago)

Thanks for the tek! I've done 4 or 5 batches using your method with some slight variations and it's practically flawless. The recipe and stick blender is key. I'm cheap and lazy so i use 3oz souffle cups instead of petri's. They take up more space vertically but it works well and i don't have to mess with parafilm. I adopted someone else's idea to deal with condensation. I lay all of the cups out and place a sheet cake pan filled with hot water on top. This way all of the cups cool slowly and the condensation is absorbed in to the agar before it cools. Thanks Again!


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InvisibleLovage
Full Time Ding Dong
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Registered: 04/22/19
Posts: 380
Re: D3's Perfect transfers and agar TEK. [Re: GoldenBoy]
    #27317842 - 05/21/21 05:51 PM (2 years, 8 months ago)

I for sure will be trying this out. your dishes are super beautiful!!!

Question. are those dishes vented or not vented? Or does that even matter.

Also in the video of you taking the punch tool samples, that band sounded like Cynic. Is that in fact who that is?


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Dear God, did you see all the boobs on that guy…


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: D3's Perfect transfers and agar TEK. [Re: Lovage]
    #27318014 - 05/21/21 08:31 PM (2 years, 8 months ago)

I believe it was ghost


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OfflineForgottenone
Stranger
Registered: 05/22/21
Posts: 2
Last seen: 2 years, 8 months
Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27318324 - 05/22/21 06:03 AM (2 years, 8 months ago)

Great info thank you for the write up


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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,396
Loc: Germany Flag
Re: D3's Perfect transfers and agar TEK. [Re: Lovage]
    #27318354 - 05/22/21 06:35 AM (2 years, 8 months ago)

Quote:

Lovage said:
that band sounded like Cynic. Is that in fact who that is?




Yeah, that`s "Year Zero" from Ghost B.C. - Infestissumam.

                  :cookiemonster:


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InvisibleLovage
Full Time Ding Dong
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Registered: 04/22/19
Posts: 380
Re: D3's Perfect transfers and agar TEK. [Re: Goatrider]
    #27319686 - 05/23/21 08:00 AM (2 years, 8 months ago)

Quote:

Goatrider said:
Quote:

Lovage said:
that band sounded like Cynic. Is that in fact who that is?




Yeah, that`s "Year Zero" from Ghost B.C. - Infestissumam.

                  :cookiemonster:



👍🏼


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Dear God, did you see all the boobs on that guy…


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InvisibleLovage
Full Time Ding Dong
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Registered: 04/22/19
Posts: 380
Re: D3's Perfect transfers and agar TEK. [Re: Lovage]
    #27325321 - 05/27/21 02:41 PM (2 years, 7 months ago)

sorry guys i have screwed this post up a few times now so I am posting it again and hoping i get it right.

DO NOT FOR ANY REASON SHAKE THE MEDIA BOTTLE WITH HOT SHIT IN IT!!!!!!

I figured this out the hard way. It is not only messy, IT HURTS!!


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Dear God, did you see all the boobs on that guy…


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OfflineB H O
Male


Registered: 03/17/13
Posts: 675
Last seen: 1 year, 21 days
Re: D3's Perfect transfers and agar TEK. [Re: Lovage]
    #27337848 - 06/06/21 05:14 PM (2 years, 7 months ago)

This has probably been asked before but after you take say 3 or 4 transfers from a nice looking dish, what do you do with the rest of the myc on that dish? is it still usable in other projects or grows?? or do you just trash the rest of the dish at this point? (referring to the plates you showed in the example video)


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: D3's Perfect transfers and agar TEK. [Re: B H O]
    #27337869 - 06/06/21 05:31 PM (2 years, 7 months ago)

If it looks clean I toss the rest to grain or throw plate in fridge as a backup


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OfflineIrishmule169
Hunter
Other


Registered: 10/21/19
Posts: 212
Loc: McKenna’s house
Last seen: 1 year, 1 day
Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc] * 1
    #27347235 - 06/13/21 08:42 PM (2 years, 7 months ago)

I use this to mix up my agar in a media bottle with the stir bar in it.. heat it up to 160 degrees and blend the shit out of it till it’s sediment free and then add the color !!

https://www.amazon.com/dp/B078HY1TSF/ref=cm_sw_em_r_mt_dp_J6B8P70CV661A8J0D9P5?_encoding=UTF8&psc=1





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Edited by Irishmule169 (06/13/21 08:45 PM)


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OfflinepossiblynotJack
Stranger
Registered: 08/01/20
Posts: 7
Last seen: 3 months, 9 hours
Re: D3's Perfect transfers and agar TEK. [Re: Irishmule169]
    #27353606 - 06/18/21 12:59 PM (2 years, 7 months ago)

After you've completed a transfer, where do you store the petri dishes?


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: D3's Perfect transfers and agar TEK. [Re: possiblynotJack]
    #27353669 - 06/18/21 01:29 PM (2 years, 7 months ago)

On a shelf


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InvisibleElonMuskrat
I Throw Bags Out For A Living
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Registered: 06/30/09
Posts: 167
Re: D3's Perfect transfers and agar TEK. [Re: verum subsequentis]
    #27377676 - 07/07/21 01:57 PM (2 years, 6 months ago)

I will deffs have to switch to this method!


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InvisibleLovage
Full Time Ding Dong
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Registered: 04/22/19
Posts: 380
Re: D3's Perfect transfers and agar TEK. [Re: ElonMuskrat]
    #27377711 - 07/07/21 02:22 PM (2 years, 6 months ago)

I am not sure if it is just my lack of fine motor skill or something else horribly wrong with my hands but i have a hard time getting the plugs off of the exacto blade. My last couple of transfers I used a discarded dental pick with amazing success. they don't look as good as these but it helped me a ton.


--------------------
Dear God, did you see all the boobs on that guy…


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Offlinefaroutdd
Stranger

Registered: 07/10/21
Posts: 1
Last seen: 2 years, 6 months
Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27384957 - 07/12/21 07:37 PM (2 years, 6 months ago)

First post on shroomery! So, I was going to follow your TEK ordered some dishes but I wasn't paying attention to size and got 60 MM. What size plates were you using?


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