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InvisibleDoctor Mario
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Re: D3's Perfect transfers and agar TEK. [Re: falconman]
    #27173134 - 01/27/21 03:14 PM (3 years, 15 hours ago)

Quote:

falconman said:
Quote:

Doctor Mario said:
I just got a case of these and wasn't disappointed.
https://www.amazon.com/Thomas-7453-Petri-Stackable-Sterile/dp/B01MY2WY3S





Ill give those a shot when I make an order... i usually went from carolina scientific and their falcon plates(ayyy username OP), but $50 isn't an insignificant difference




Thats what I was getting until my last order with them. They didn't send me falcons. They sent me some cheap ass garbage in a plastic bag that was about as thick as a grocery bag.


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OfflinePitcherCrab
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27173159 - 01/27/21 03:27 PM (3 years, 15 hours ago)

Quote:

D3monic said:
Quote:

PitcherCrab said:
I was able to pick up some of the Celltreat with the grip rings on Amazon. They said they'd ship in 1-3 weeks but ended up shipping the next day. The case was $110 shipped.

Was just looking at that parafilm on Amazon too... for some reason they can't ship to my location. WTF??




That’s weird.. can’t imagine that’s a locationally restricted item.




Its finnnnnnnne. *Stomps back to the kitchen to cut up more Glad cling wrap.*

I was able to find some that would ship to me on ebay, but it was 50% more expensive, so I'll probably hold off on that. The cling wrap isn't so bad for me.


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



Edited by PitcherCrab (01/27/21 03:28 PM)


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OfflinePitcherCrab
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Re: D3's Perfect transfers and agar TEK. [Re: PitcherCrab]
    #27173324 - 01/27/21 05:45 PM (3 years, 13 hours ago)

Scratch that. I found a vendor on ebay that has it!

D3, just to clarify about how you use it, do you keep it on the roll while you're applying it to the dishes? I'm imagining I'd use it the same way I use the little rolls of cling wrap: Anchor one end with my left hand while holding the roll in my right hand and rotate the plate while stretching it around the edge. Once its fully around the plate tear it off. (I hope that makes sense)


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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InvisibleD3_Myc
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Re: D3's Perfect transfers and agar TEK. [Re: PitcherCrab]
    #27173350 - 01/27/21 06:05 PM (3 years, 12 hours ago)

Exactly how ya do it, has a lil stretch to it but not a lot. Just twist it around the plate and stretch to tear at the end. It’s almost waxy and will stick to itself with a lil stretch.


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OfflinePitcherCrab
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27173355 - 01/27/21 06:09 PM (3 years, 12 hours ago)

Perfect. Thanks for the clarification! I'm going to pick some up. Seems like a big step up from cling wrap and just as easy to use. :grin:


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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InvisibleBenson
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Re: D3's Perfect transfers and agar TEK. [Re: PitcherCrab]
    #27176348 - 01/29/21 02:26 PM (2 years, 11 months ago)



Found this single stainless steel straw for less than $3 and wow it feels legit! Thanks for the inspo D3, I'll post progress pics  :rockon:


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OfflinePitcherCrab
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Re: D3's Perfect transfers and agar TEK. [Re: Benson]
    #27180065 - 01/31/21 10:19 PM (2 years, 11 months ago)

I picked up a 6 pack of that parafilm you recommended and I am in love! Thanks so much for the recommendation! It's far above and beyond my expectations. I also bought some brass 1/4" tubing and made a handle for it from 2 part epoxy puddy. I'll be posting a photo once I've finished sanding it tomorrow. :grin:
:leocheers:


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



Edited by PitcherCrab (01/31/21 10:27 PM)


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InvisibleD3_Myc
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Re: D3's Perfect transfers and agar TEK. [Re: PitcherCrab]
    #27180107 - 01/31/21 10:54 PM (2 years, 11 months ago)

Oh nice, good idea!

I been meaning to make a silicone mold so I can pour some acrylic handles but haven’t gotten around to it. The putty is a good idea.


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OfflinePitcherCrab
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27180122 - 01/31/21 11:05 PM (2 years, 11 months ago)

I had a similar idea. I've done a bit of silicone mold making and will be getting a 3D printer this month. So I was thinking I may try 3D printing a handle and making a mold for acrylic or just straight printing the mold instead of the two step process. In the meantime I think the puddy is going to work perfectly!


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Re: D3's Perfect transfers and agar TEK. [Re: PitcherCrab]
    #27181421 - 02/01/21 05:12 PM (2 years, 11 months ago)

Alright, here is the transfer tool I made! I'm wondering if I need to put a hole in it somewhere like yours has D3, to prevent vacuum so the agar plug doesn't get stuck in the tube. Thoughts?



--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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InvisibleD3_Myc
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Re: D3's Perfect transfers and agar TEK. [Re: PitcherCrab]
    #27181430 - 02/01/21 05:18 PM (2 years, 11 months ago)

That looks better than I pictured even. I’d put one in the ass at the other end the tube so you can pour iso through it maybe?


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OfflinePitcherCrab
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27181481 - 02/01/21 05:51 PM (2 years, 11 months ago)

Thanks! I think it came out super nicely. I'd like to get some higher grit sand paper to make the finish a little smoother, but otherwise I'm very pleased. Does your custom tool have a hole in the back for iso? I did cut a few little notches in the tube for the epoxy to smoosh into towards the end as I was afraid it might just slide out if I didn't. I could drill a hole through the back end but I'd have to aim really well.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineCerealkilla0426
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Re: D3's Perfect transfers and agar TEK. [Re: PitcherCrab]
    #27181804 - 02/01/21 09:16 PM (2 years, 11 months ago)

Just start with a small drill bit and increase to desired size should be a lot easier that way use something sharp to make an indent dead center and start the smallest bit you have


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Offlinefalconman
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Re: D3's Perfect transfers and agar TEK. [Re: Cerealkilla0426]
    #27183582 - 02/02/21 08:18 PM (2 years, 11 months ago)

@D3, any pointers for getting the bubbles out of agar pours? And condensation out of plates? I get most of it out but some plates are just stubborn and even 24hrs in front of hepa fan/4 days in flow hood wont get the condensation out of some.


--------------------
If any of my posted info is incorrect or questionable,please DO correct me. I strive to always learn new or better ways and information




Grower off all things nature!
things currently growing I am proud of:
50+ trichocereus named cultivars
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30+ varieties of herbs, berries, beans, and vegetables
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InvisibleD3_Myc
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Re: D3's Perfect transfers and agar TEK. [Re: falconman]
    #27183620 - 02/02/21 08:31 PM (2 years, 11 months ago)

For bubbles, do not shake or do anything more than a gentle swirl with your media bottle.

As for the condensation, pour as cool as possible say 125-135f,  avoid temp swings. Worst case before using plate just tip the excess water onto a paper towel or something. I can’t imagine it still being there after days in front of a hood. These are unwrapped petris right?


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OfflinePitcherCrab
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27183648 - 02/02/21 08:41 PM (2 years, 11 months ago)

Is pouring extra water off the plate a good way of getting rid of condensation if I don't have time to leave my plates in front of my flow hood for days? My hood is in a main work area of mine so unfortunately I don't have the luxury of time. Also just got those Celltreat plates with grip rings. These things are niiiiice, big upgrade.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



Edited by PitcherCrab (02/02/21 08:42 PM)


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Offlinefalconman
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc] * 1
    #27183680 - 02/02/21 09:00 PM (2 years, 11 months ago)

Yep unwrapped perris, at this point its mostly giant drops on the lid


--------------------
If any of my posted info is incorrect or questionable,please DO correct me. I strive to always learn new or better ways and information




Grower off all things nature!
things currently growing I am proud of:
50+ trichocereus named cultivars
B. Caapi that has died and came back twice
30+ varieties of herbs, berries, beans, and vegetables
psilocybe cubensis


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Offlinehogstomper
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Re: D3's Perfect transfers and agar TEK. [Re: EZPZyaBZ]
    #27191252 - 02/07/21 09:09 AM (2 years, 11 months ago)

Quote:

EZPZyaBZ said:


This was inspired by you D3monic. Thx bud. Agar work has been a dream since I made this little guy.

:cheers:




this is the coolest invention ever--i was just fantasizing about how it would be cool to make something like this.  very nice work mon


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OfflinePitcherCrab
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Re: D3's Perfect transfers and agar TEK. [Re: hogstomper]
    #27198480 - 02/11/21 12:50 AM (2 years, 11 months ago)

I gotta hand it to you D3... this tek is *legit*. I just poured some of my new Celltreat grip ring dishes and I don’t think I’ve ever gotten such beautiful clear plates. Once I wrap them tomorrow I’ll post some photos. Thanks for all your hard work and experimentation. I give it PitcherCrab’s 5 claws of approval!  :amanita2:  (And sorry for blowing up your thread so much!)


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Offlinetomcards
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Re: D3's Perfect transfers and agar TEK. [Re: PitcherCrab]
    #27198759 - 02/11/21 07:38 AM (2 years, 11 months ago)

D3monics tek rules. 

A word of warning.  Do not use more than one drop of dye per media bottle.  Much like tripping, I fell in love with the colors.  I put 3 drops of blue dye into a bottle and poured plates with it. 

I did a T2 transfer of Melmak onto the plate and saw no growth for three days.  I usually see growth in about 12 hours.  I thought I killed it.  It eventually grew, but the growth rate was incredibly slow and tomentose. 

I poured more plates, with one drop of dye per media bottle.  I excised a plug of Melmak from the beautiful, though dastardly plate and transferred it to the new plate.  Growth appeared within 12 hours and Melmak is now flourishing.  Whew! 

Bottom line.  If you use more than one drop of dye per media bottle, it will inhibit growth while encouraging tomentose mycelium. 

Don't do it.


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