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 Benson
The Kidd
Registered: 09/29/20
Posts: 25
Last seen: 1 day, 18 hours
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 Re: D3's Perfect transfers and agar TEK.  [Re: D3monic]
#27085522 - 12/12/20 01:15 PM (1 month, 11 days ago) |
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Quote:
D3monic said:
I like pastys for swab dunks, gives you a little more head room and moisture for stubborn spores.
Relative noob here - would you mind explaining how moisture effects 'stubborn spores'?
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 D3monic
Weeb Trash
Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
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Re: D3's Perfect transfers and agar TEK. [Re: Benson]
#27085569 - 12/12/20 01:41 PM (1 month, 11 days ago) |
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It rehydrates the spores
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hogstomper
Strangler
Registered: 03/11/05
Posts: 71
Last seen: 8 days, 10 hours
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Re: D3's Perfect transfers and agar TEK. [Re: Ababyphoenix]
#27087018 - 12/13/20 11:33 AM (1 month, 10 days ago) |
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Sorry if this has been answered, but I noticed the recipe says 1L water, but 1 of the bottles in original recipe pic has 250 ml. 250ml X 3 = 750ml. Are you losing water during the initial boil? Should I end up with 1L in the bottles before pc'ing? I had slightly less after boiling so I compensated with a few dashes of water to each container to end up with a total of 1L agar mix before PC-ing.
For instance, I noticed Bod talking about having yr media bottle filled to 400ml and doing 20 plates. I can do barely exactly 20 with 500 ML--am I filling the plates too thickly and ending up with thick watery agar plates that condensate to hell and back?
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no justice no peace
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D3monic
Weeb Trash
Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
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Re: D3's Perfect transfers and agar TEK. [Re: hogstomper]
#27087154 - 12/13/20 01:27 PM (1 month, 10 days ago) |
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I don’t really boil long enough to loose anything more than a negligible amount. I don’t necessarily pour an exactly even amount in each media bottle. One may be 250 another may be 350... I just eyeball split. Since All the ingredients are already mixed theres no need to be exact. 1l should be enough to pour 40 plates and still have a little left over. You may be pouring a little too thick.
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GrinchGrower
N00B
 
Registered: 10/02/20
Posts: 634
Last seen: 1 hour, 7 minutes
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Re: D3's Perfect transfers and agar TEK. [Re: D3monic]
#27087255 - 12/13/20 03:11 PM (1 month, 10 days ago) |
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I have only poured a handful of times, but I seem to get roughly 25 plates from 500ml (20ml per dish on average).
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tomcards
Registered: 04/24/15
Posts: 324
Loc: San Francisco
Last seen: 7 hours, 36 minutes
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Re: D3's Perfect transfers and agar TEK. [Re: GrinchGrower]
#27090418 - 12/15/20 02:07 PM (1 month, 8 days ago) |
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Guys,
After growing Rustywhyte and B+ on the beautiful green agar, I tried the orange dye and the charcoal. I made the T3 transfers yesterday. The myc is already happily growing.
Next time, I need to blend my charcoal better. Otherwise, I dig these pretty plates.
Thanks again D3monic!
Tom
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D3monic
Weeb Trash
Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
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Re: D3's Perfect transfers and agar TEK. [Re: tomcards]
#27090532 - 12/15/20 03:23 PM (1 month, 8 days ago) |
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Very Halloween, I love it!
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hogstomper
Strangler
Registered: 03/11/05
Posts: 71
Last seen: 8 days, 10 hours
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Re: D3's Perfect transfers and agar TEK. [Re: D3monic]
#27102691 - 12/22/20 05:44 PM (1 month, 1 day ago) |
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tried this and wound up with very nice clear dishes--however:
-many plates have a few bubbles, which I don't mind but can be a little annoying
-some plates have like a big drop of standing water that rolls around when tilted--very distressing! I can't figure out what caused that? they do seem a bit soft
-i used a stainless steel straw to cut little circles, but my pours are so thick they ended up stuck inside the straw, which was a bummer--I switched back to just cuts for now, though I want to find a better tool and technique for this.
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no justice no peace
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Kabluee
Stranger
Registered: 10/03/20
Posts: 82
Last seen: 6 hours, 52 minutes
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Re: D3's Perfect transfers and agar TEK. [Re: hogstomper]
#27114337 - 12/29/20 04:16 PM (25 days, 6 hours ago) |
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Hey do you think this will be ok to use?
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PocketKafka
Lurker
Registered: 12/05/16
Posts: 14
Loc: US
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Re: D3's Perfect transfers and agar TEK. [Re: Kabluee]
#27117513 - 12/31/20 05:56 AM (23 days, 17 hours ago) |
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This thread really makes me want to take up mycology photography. Very nice work 
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figgusfiddus said:
"Man, this guy has a fuck-ton of lizards."
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dingdonger22
Stranger
Registered: 12/14/20
Posts: 16
Last seen: 5 hours, 54 minutes
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Re: D3's Perfect transfers and agar TEK. [Re: PocketKafka]
#27120181 - 01/01/21 01:15 PM (22 days, 9 hours ago) |
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D3monic, thank you for the great tek!
Does this look ok? It's been about 6 days since transfer and I'm getting worried.
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D3monic
Weeb Trash
Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
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Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22]
#27120201 - 01/01/21 01:31 PM (22 days, 9 hours ago) |
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Variety, temps and agar recipe can give varying results in growth speed. That leading clear edge looks fine, It’s probably just digging in. A softer or more nutritious recipe will see that on occasion.
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dingdonger22
Stranger
Registered: 12/14/20
Posts: 16
Last seen: 5 hours, 54 minutes
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Re: D3's Perfect transfers and agar TEK. [Re: D3monic]
#27120209 - 01/01/21 01:36 PM (22 days, 9 hours ago) |
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Thank you so much for the reply, D3. This was done at 2% agar and 2% LME because thats how I learned. Will try the amount of LME you recommend.
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tomcards
Registered: 04/24/15
Posts: 324
Loc: San Francisco
Last seen: 7 hours, 36 minutes
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Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22] 1
#27121777 - 01/02/21 11:58 AM (21 days, 11 hours ago) |
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My menagerie of Rustywhyte, B+, Ban Phang Ka and Melmac on D3monic's wonderful agar.
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Land Trout
Stranger
Registered: 01/08/18
Posts: 443
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Re: D3's Perfect transfers and agar TEK. [Re: tomcards]
#27121827 - 01/02/21 12:45 PM (21 days, 10 hours ago) |
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Following
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Frozen Jedi
nooberist
Registered: 10/17/20
Posts: 26
Last seen: 9 days, 23 hours
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Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22]
#27122927 - 01/03/21 12:20 AM (20 days, 22 hours ago) |
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Quote:
dingdonger22 said:
D3monic, thank you for the great tek!
Does this look ok? It's been about 6 days since transfer and I'm getting worried.
what's the history behind this transfer? T1? T2? species?
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dingdonger22
Stranger
Registered: 12/14/20
Posts: 16
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Re: D3's Perfect transfers and agar TEK. [Re: Frozen Jedi]
#27131819 - 01/06/21 11:50 PM (16 days, 23 hours ago) |
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It is a third transfer from a Mazatapec streak plate!
Included some photos I took on Monday. The one on the purple agar is the plate these were transferred from.
  
I'm worried about this fuzzy growth on top, is that normal? I can tell underneath there is rhizomorphic (as shown in the last photo around 6 o'clock area to ~8:45 o'clock area) growth.
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piq
Stranger
Registered: 08/04/20
Posts: 25
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Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22]
#27145967 - 01/13/21 03:29 PM (10 days, 7 hours ago) |
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Great tek, really was not expecting this level of clarity for my first attempt with agar.
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D3monic
Weeb Trash
Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
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Re: D3's Perfect transfers and agar TEK. [Re: piq]
#27146093 - 01/13/21 04:58 PM (10 days, 6 hours ago) |
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Quote:
dingdonger22 said:
It is a third transfer from a Mazatapec streak plate!
Included some photos I took on Monday. The one on the purple agar is the plate these were transferred from.
I'm worried about this fuzzy growth on top, is that normal? I can tell underneath there is rhizomorphic (as shown in the last photo around 6 o'clock area to ~8:45 o'clock area) growth.
Looks like standard tomentos growth. I don’t like the fluffy myc myself. Always makes me second guess it. But from what I can see it looks healthy.
Quote:
piq said:
Great tek, really was not expecting this level of clarity for my first attempt with agar.
Looking good piq!
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GrinchGrower
N00B
Registered: 10/02/20
Posts: 634
Last seen: 1 hour, 7 minutes
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Re: D3's Perfect transfers and agar TEK. [Re: D3monic]
#27146238 - 01/13/21 06:29 PM (10 days, 4 hours ago) |
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