Home | Community | Message Board


Zamnesia.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Amazon Agar

Jump to first unread post. Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | Next >
OfflineBenson
The Kidd


Registered: 09/29/20
Posts: 25
Last seen: 1 day, 18 hours
Re: D3's Perfect transfers and agar TEK. [Re: D3monic]
    #27085522 - 12/12/20 01:15 PM (1 month, 11 days ago)

Quote:

D3monic said:
I like pastys for swab dunks, gives you a little more head room and moisture for stubborn spores.




Relative noob here - would you mind explaining how moisture effects 'stubborn spores'?


--------------------
[|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||]
[|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||]
[|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||]


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleD3monicS
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
Re: D3's Perfect transfers and agar TEK. [Re: Benson]
    #27085569 - 12/12/20 01:41 PM (1 month, 11 days ago)

It rehydrates the spores


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinehogstomper
Strangler


Registered: 03/11/05
Posts: 71
Last seen: 8 days, 10 hours
Re: D3's Perfect transfers and agar TEK. [Re: Ababyphoenix]
    #27087018 - 12/13/20 11:33 AM (1 month, 10 days ago)

Sorry if this has been answered, but I noticed the recipe says 1L water, but 1 of the bottles in original recipe pic has 250 ml.  250ml X 3 = 750ml.  Are you losing water during the initial boil?  Should I end up with 1L in the bottles before pc'ing?  I had slightly less after boiling so I compensated with a few dashes of water to each container to end up with a total of 1L agar mix before PC-ing. 

For instance, I noticed Bod talking about having yr media bottle filled to 400ml and doing 20 plates.  I can do barely exactly 20 with 500 ML--am I filling the plates too thickly and ending up with thick watery agar plates that condensate to hell and back?


--------------------
no justice no peace


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleD3monicS
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
Re: D3's Perfect transfers and agar TEK. [Re: hogstomper]
    #27087154 - 12/13/20 01:27 PM (1 month, 10 days ago)

I don’t really boil long enough to loose anything more than a negligible amount. I don’t necessarily pour an exactly even amount in each media bottle. One may be 250 another may be 350... I just eyeball split. Since All the ingredients are already mixed theres no need to be exact. 1l should be enough to pour 40 plates and still have a little left over. You may be pouring a little too thick.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineGrinchGrower
N00B
Male User Gallery


Registered: 10/02/20
Posts: 634
Last seen: 1 hour, 7 minutes
Re: D3's Perfect transfers and agar TEK. [Re: D3monic]
    #27087255 - 12/13/20 03:11 PM (1 month, 10 days ago)

I have only poured a handful of times, but I seem to get roughly 25 plates from 500ml (20ml per dish on average).


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinetomcards
Male


Registered: 04/24/15
Posts: 324
Loc: San Francisco
Last seen: 7 hours, 36 minutes
Re: D3's Perfect transfers and agar TEK. [Re: GrinchGrower]
    #27090418 - 12/15/20 02:07 PM (1 month, 8 days ago)

Guys,

After growing Rustywhyte and B+ on the beautiful green agar, I tried the orange dye and the charcoal.  I made the T3 transfers yesterday.  The myc is already happily growing.





Next time, I need to blend my charcoal better.  Otherwise, I dig these pretty plates.


Thanks again D3monic!


Tom


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleD3monicS
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
Re: D3's Perfect transfers and agar TEK. [Re: tomcards]
    #27090532 - 12/15/20 03:23 PM (1 month, 8 days ago)

Very Halloween, I love it!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinehogstomper
Strangler


Registered: 03/11/05
Posts: 71
Last seen: 8 days, 10 hours
Re: D3's Perfect transfers and agar TEK. [Re: D3monic]
    #27102691 - 12/22/20 05:44 PM (1 month, 1 day ago)

tried this and wound up with very nice clear dishes--however:

-many plates have a few bubbles, which I don't mind but can be a little annoying

-some plates have like a big drop of standing water that rolls around when tilted--very distressing!  I can't figure out what caused that? they do seem a bit soft

-i used a stainless steel straw to cut little circles, but my pours are so thick they ended up stuck inside the straw, which was a bummer--I switched back to just cuts for now, though I want to find a better tool and technique for this.


--------------------
no justice no peace


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineKabluee
Stranger
Registered: 10/03/20
Posts: 82
Last seen: 6 hours, 52 minutes
Re: D3's Perfect transfers and agar TEK. [Re: hogstomper]
    #27114337 - 12/29/20 04:16 PM (25 days, 6 hours ago)

Hey do you think this will be ok to use?



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisiblePocketKafka
Lurker
Male


Registered: 12/05/16
Posts: 14
Loc: US
Re: D3's Perfect transfers and agar TEK. [Re: Kabluee]
    #27117513 - 12/31/20 05:56 AM (23 days, 17 hours ago)

This thread really makes me want to take up mycology photography. Very nice work :thumbup:


--------------------
figgusfiddus said:
"Man, this guy has a fuck-ton of lizards."


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinedingdonger22
Stranger
Registered: 12/14/20
Posts: 16
Last seen: 5 hours, 54 minutes
Re: D3's Perfect transfers and agar TEK. [Re: PocketKafka]
    #27120181 - 01/01/21 01:15 PM (22 days, 9 hours ago)

D3monic, thank you for the great tek!

Does this look ok? It's been about 6 days since transfer and I'm getting worried.



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleD3monicS
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22]
    #27120201 - 01/01/21 01:31 PM (22 days, 9 hours ago)

Variety, temps and agar recipe can give  varying results in growth speed. That leading clear edge looks fine, It’s probably just digging in. A softer or more nutritious recipe will see that on occasion.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinedingdonger22
Stranger
Registered: 12/14/20
Posts: 16
Last seen: 5 hours, 54 minutes
Re: D3's Perfect transfers and agar TEK. [Re: D3monic]
    #27120209 - 01/01/21 01:36 PM (22 days, 9 hours ago)

Thank you so much for the reply, D3. This was done at 2% agar and 2% LME because thats how I learned. Will try the amount of LME you recommend.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinetomcards
Male


Registered: 04/24/15
Posts: 324
Loc: San Francisco
Last seen: 7 hours, 36 minutes
Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22] * 1
    #27121777 - 01/02/21 11:58 AM (21 days, 11 hours ago)

My menagerie of Rustywhyte, B+, Ban Phang Ka and Melmac on D3monic's wonderful agar.




Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineLand Trout
Stranger
Male User Gallery


Registered: 01/08/18
Posts: 443
Last seen: 1 hour, 20 minutes
Re: D3's Perfect transfers and agar TEK. [Re: tomcards]
    #27121827 - 01/02/21 12:45 PM (21 days, 10 hours ago)

Following


--------------------


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineFrozen Jedi
nooberist
Male

Registered: 10/17/20
Posts: 26
Last seen: 9 days, 23 hours
Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22]
    #27122927 - 01/03/21 12:20 AM (20 days, 22 hours ago)

Quote:

dingdonger22 said:
D3monic, thank you for the great tek!

Does this look ok? It's been about 6 days since transfer and I'm getting worried.





what's the history behind this transfer? T1? T2? species?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinedingdonger22
Stranger
Registered: 12/14/20
Posts: 16
Last seen: 5 hours, 54 minutes
Re: D3's Perfect transfers and agar TEK. [Re: Frozen Jedi]
    #27131819 - 01/06/21 11:50 PM (16 days, 23 hours ago)

It is a third transfer from a Mazatapec streak plate!

Included some photos I took on Monday. The one on the purple agar is the plate these were transferred from.



I'm worried about this fuzzy growth on top, is that normal? I can tell underneath there is rhizomorphic (as shown in the last photo around 6 o'clock area to ~8:45 o'clock area) growth.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinepiq
Stranger
Registered: 08/04/20
Posts: 25
Last seen: 3 hours, 57 minutes
Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22]
    #27145967 - 01/13/21 03:29 PM (10 days, 7 hours ago)

Great tek, really was not expecting this level of clarity for my first attempt with agar.



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleD3monicS
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 2,678
Loc: Year Zero
Re: D3's Perfect transfers and agar TEK. [Re: piq]
    #27146093 - 01/13/21 04:58 PM (10 days, 6 hours ago)

Quote:

dingdonger22 said:
It is a third transfer from a Mazatapec streak plate!

Included some photos I took on Monday. The one on the purple agar is the plate these were transferred from.



I'm worried about this fuzzy growth on top, is that normal? I can tell underneath there is rhizomorphic (as shown in the last photo around 6 o'clock area to ~8:45 o'clock area) growth.




Looks like standard tomentos growth. I don’t like the fluffy myc myself. Always makes me second guess it. But from what I can see it looks healthy.

Quote:

piq said:
Great tek, really was not expecting this level of clarity for my first attempt with agar.






Looking good piq!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineGrinchGrower
N00B
Male User Gallery


Registered: 10/02/20
Posts: 634
Last seen: 1 hour, 7 minutes
Re: D3's Perfect transfers and agar TEK. [Re: D3monic]
    #27146238 - 01/13/21 06:29 PM (10 days, 4 hours ago)

Cross posting from LAGM'21, because this was the first time I broke out the immersion blender that D3 recommends when prepping agar.




It helped clear up those last few specks of LME that were hanging around and really improved the clarity.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | Next >

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Amazon Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 3,599 8 12/13/19 08:16 PM
by mushboy
* transferring agar meatman 1,765 3 10/02/02 03:13 PM
by tripndicular
* Re: Peroxidated Agar Tek sparks 3,014 6 05/08/00 02:43 AM
by Anonymous
* agar tek? the man 2,016 4 12/16/02 12:45 PM
by the man
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 15,775 38 05/11/01 01:08 PM
by MAIA
* Agar questions-I may have goofed blahblahblah 2,427 6 01/11/02 04:58 PM
by ar393
* Mycelium-only tek, Please Help! FaminedShaman 2,009 4 05/06/01 08:49 AM
by egghead
* Antibac Agar, DIY! superduper 3,913 13 12/04/02 04:20 PM
by superduper

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
13,677 topic views. 28 members, 149 guests and 22 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Kratom Eye
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.045 seconds spending 0.001 seconds on 14 queries.