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InvisibleBenson
The Kidd ⭐


Registered: 09/29/20
Posts: 837
Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27085522 - 12/12/20 11:15 AM (3 years, 1 month ago)

Quote:

D3monic said:
I like pastys for swab dunks, gives you a little more head room and moisture for stubborn spores.




Relative noob here - would you mind explaining how moisture effects 'stubborn spores'?


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: D3's Perfect transfers and agar TEK. [Re: Benson]
    #27085569 - 12/12/20 11:41 AM (3 years, 1 month ago)

It rehydrates the spores


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Offlinehogstomper
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Registered: 03/11/05
Posts: 100
Last seen: 2 years, 2 months
Re: D3's Perfect transfers and agar TEK. [Re: Ababyphoenix]
    #27087018 - 12/13/20 09:33 AM (3 years, 1 month ago)

Sorry if this has been answered, but I noticed the recipe says 1L water, but 1 of the bottles in original recipe pic has 250 ml.  250ml X 3 = 750ml.  Are you losing water during the initial boil?  Should I end up with 1L in the bottles before pc'ing?  I had slightly less after boiling so I compensated with a few dashes of water to each container to end up with a total of 1L agar mix before PC-ing. 

For instance, I noticed Bod talking about having yr media bottle filled to 400ml and doing 20 plates.  I can do barely exactly 20 with 500 ML--am I filling the plates too thickly and ending up with thick watery agar plates that condensate to hell and back?


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no justice no peace.  black trans lives matter.


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: D3's Perfect transfers and agar TEK. [Re: hogstomper]
    #27087154 - 12/13/20 11:27 AM (3 years, 1 month ago)

I don’t really boil long enough to loose anything more than a negligible amount. I don’t necessarily pour an exactly even amount in each media bottle. One may be 250 another may be 350... I just eyeball split. Since All the ingredients are already mixed theres no need to be exact. 1l should be enough to pour 40 plates and still have a little left over. You may be pouring a little too thick.


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OfflineGrinchGrower
N00B
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Registered: 10/02/20
Posts: 682
Last seen: 2 years, 8 months
Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27087255 - 12/13/20 01:11 PM (3 years, 1 month ago)

I have only poured a handful of times, but I seem to get roughly 25 plates from 500ml (20ml per dish on average).


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Offlinetomcards
Male


Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
Re: D3's Perfect transfers and agar TEK. [Re: GrinchGrower]
    #27090418 - 12/15/20 12:07 PM (3 years, 1 month ago)

Guys,

After growing Rustywhyte and B+ on the beautiful green agar, I tried the orange dye and the charcoal.  I made the T3 transfers yesterday.  The myc is already happily growing.





Next time, I need to blend my charcoal better.  Otherwise, I dig these pretty plates.


Thanks again D3monic!


Tom


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: D3's Perfect transfers and agar TEK. [Re: tomcards]
    #27090532 - 12/15/20 01:23 PM (3 years, 1 month ago)

Very Halloween, I love it!


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Offlinehogstomper
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Registered: 03/11/05
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27102691 - 12/22/20 03:44 PM (3 years, 1 month ago)

tried this and wound up with very nice clear dishes--however:

-many plates have a few bubbles, which I don't mind but can be a little annoying

-some plates have like a big drop of standing water that rolls around when tilted--very distressing!  I can't figure out what caused that? they do seem a bit soft

-i used a stainless steel straw to cut little circles, but my pours are so thick they ended up stuck inside the straw, which was a bummer--I switched back to just cuts for now, though I want to find a better tool and technique for this.


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no justice no peace.  black trans lives matter.


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OfflineKabluee
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Registered: 10/03/20
Posts: 210
Last seen: 2 months, 3 days
Re: D3's Perfect transfers and agar TEK. [Re: hogstomper]
    #27114337 - 12/29/20 02:16 PM (3 years, 29 days ago)

Hey do you think this will be ok to use?



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InvisiblePocketKafka
Lurker
Male


Registered: 12/05/16
Posts: 14
Loc: US
Re: D3's Perfect transfers and agar TEK. [Re: Kabluee]
    #27117513 - 12/31/20 03:56 AM (3 years, 28 days ago)

This thread really makes me want to take up mycology photography. Very nice work :thumbup:


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figgusfiddus said:
"Man, this guy has a fuck-ton of lizards."


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Offlinedingdonger22
Stranger
Registered: 12/14/20
Posts: 35
Last seen: 2 years, 9 months
Re: D3's Perfect transfers and agar TEK. [Re: PocketKafka]
    #27120181 - 01/01/21 11:15 AM (3 years, 26 days ago)

D3monic, thank you for the great tek!

Does this look ok? It's been about 6 days since transfer and I'm getting worried.



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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22]
    #27120201 - 01/01/21 11:31 AM (3 years, 26 days ago)

Variety, temps and agar recipe can give  varying results in growth speed. That leading clear edge looks fine, It’s probably just digging in. A softer or more nutritious recipe will see that on occasion.


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Offlinedingdonger22
Stranger
Registered: 12/14/20
Posts: 35
Last seen: 2 years, 9 months
Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27120209 - 01/01/21 11:36 AM (3 years, 26 days ago)

Thank you so much for the reply, D3. This was done at 2% agar and 2% LME because thats how I learned. Will try the amount of LME you recommend.


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Offlinetomcards
Male


Registered: 04/24/15
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Loc: San Francisco
Last seen: 10 months, 19 days
Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22] * 1
    #27121777 - 01/02/21 09:58 AM (3 years, 25 days ago)

My menagerie of Rustywhyte, B+, Ban Phang Ka and Melmac on D3monic's wonderful agar.




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OfflineLand TroutM
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I'm a teapot User Gallery


Registered: 01/08/18
Posts: 3,076
Last seen: 8 hours, 42 minutes
Re: D3's Perfect transfers and agar TEK. [Re: tomcards]
    #27121827 - 01/02/21 10:45 AM (3 years, 25 days ago)

Following


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OfflineFrozen Jedi
nooberist
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Registered: 10/17/20
Posts: 26
Last seen: 2 years, 11 months
Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22]
    #27122927 - 01/02/21 10:20 PM (3 years, 25 days ago)

Quote:

dingdonger22 said:
D3monic, thank you for the great tek!

Does this look ok? It's been about 6 days since transfer and I'm getting worried.





what's the history behind this transfer? T1? T2? species?


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Offlinedingdonger22
Stranger
Registered: 12/14/20
Posts: 35
Last seen: 2 years, 9 months
Re: D3's Perfect transfers and agar TEK. [Re: Frozen Jedi]
    #27131819 - 01/06/21 09:50 PM (3 years, 21 days ago)

It is a third transfer from a Mazatapec streak plate!

Included some photos I took on Monday. The one on the purple agar is the plate these were transferred from.



I'm worried about this fuzzy growth on top, is that normal? I can tell underneath there is rhizomorphic (as shown in the last photo around 6 o'clock area to ~8:45 o'clock area) growth.


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Offlinepiq
Stranger
Registered: 08/04/20
Posts: 27
Last seen: 2 years, 1 month
Re: D3's Perfect transfers and agar TEK. [Re: dingdonger22]
    #27145967 - 01/13/21 01:29 PM (3 years, 14 days ago)

Great tek, really was not expecting this level of clarity for my first attempt with agar.



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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: D3's Perfect transfers and agar TEK. [Re: piq]
    #27146093 - 01/13/21 02:58 PM (3 years, 14 days ago)

Quote:

dingdonger22 said:
It is a third transfer from a Mazatapec streak plate!

Included some photos I took on Monday. The one on the purple agar is the plate these were transferred from.



I'm worried about this fuzzy growth on top, is that normal? I can tell underneath there is rhizomorphic (as shown in the last photo around 6 o'clock area to ~8:45 o'clock area) growth.




Looks like standard tomentos growth. I don’t like the fluffy myc myself. Always makes me second guess it. But from what I can see it looks healthy.

Quote:

piq said:
Great tek, really was not expecting this level of clarity for my first attempt with agar.






Looking good piq!


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OfflineGrinchGrower
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Registered: 10/02/20
Posts: 682
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Re: D3's Perfect transfers and agar TEK. [Re: D3_Myc]
    #27146238 - 01/13/21 04:29 PM (3 years, 14 days ago)

Cross posting from LAGM'21, because this was the first time I broke out the immersion blender that D3 recommends when prepping agar.




It helped clear up those last few specks of LME that were hanging around and really improved the clarity.


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