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OfflineBlue Helix
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Inflating spawn bags without a flow hood * 1
    #26511950 - 03/01/20 08:26 PM (3 years, 11 months ago)

Today I was messing around with some stuff I already had lying around that you can easily hook up a bicycle pump to a needle to post-PC inflate spawn bags if you own the popular LC filter discs.  Recall that those discs are designed to be connected to a needle on their output and a syringe on their input.  So hooking up the needle is simple enough. 

And to connect the input side to the tubing rather than a syringe all you need is a piece of flexible tubing which will run you all of 10 cents at any hardware store.  On the input side of the filter disc, there is a lip for screwing in your syringe.  Well, if you stretch the tubing over that lip and zip tie it down, that is covered too.  And, finally, as it turns out inflatable stuff for pools often has a tubing connector that closely matches the size of this tubing, so with a standard air pump, comes an attachment to connect the flexible tubing!

The filter discs I recommend are the hydrophobic 0.22um ones which should provide a level of filtration in excess of a flow hood or comparable at least. 



One other thing: in the picture, I show vinyl tubing, but I wasn't sure if that would melt in a PC, so I replaced it with silicone tubing.  Regardless you don't really need to sterilize anything on the input side, so you can just hook up your air pump to the syringe input side of the filter disc after the disc and needle are taken out of the pressure cooker if you don't have heat-stable flexible tubing.


Edited by Blue Helix (03/01/20 08:37 PM)


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Offlinelovepotion
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Re: Inflating spawn bags without a flow hood [Re: Blue Helix]
    #26511975 - 03/01/20 08:47 PM (3 years, 11 months ago)

Blue Helix has no off switch for their Genius


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OfflinePsilosopherr
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Re: Inflating spawn bags without a flow hood [Re: Blue Helix]
    #26603105 - 04/15/20 02:09 PM (3 years, 9 months ago)

Awesome idea! I was fiddling with something very similar a while back but what you've come up with is better. :congrats:


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Re: Inflating spawn bags without a flow hood [Re: Psilosopherr]
    #26603870 - 04/15/20 07:23 PM (3 years, 9 months ago)

Rad! I did something similar with an air mattress pump and a huge polyfill prefilter... then I used a plastic cup on the pump out feed and stuck it over my .5 micron patch. Filled in about 10 seconds. No contams. But I still just hang them. I'm not seeing any slowdown from lack of air.


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Re: Inflating spawn bags without a flow hood [Re: tedoro]
    #26604037 - 04/15/20 08:35 PM (3 years, 9 months ago)

This may be a really stupid question but why inflate with all the precautions when we’re just going to PC the spawn bag after being filled with grain ???


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OfflinePsilosopherr
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Re: Inflating spawn bags without a flow hood [Re: Irishmule169]
    #26604364 - 04/15/20 10:55 PM (3 years, 9 months ago)

i believe the idea is to inflate it after PC/inoculation


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Offlinepoisoned
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Re: Inflating spawn bags without a flow hood [Re: Psilosopherr]
    #26614546 - 04/20/20 07:36 AM (3 years, 9 months ago)

Yeah, usually for g2g transfer.


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InvisibleHobbit GDF
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Re: Inflating spawn bags without a flow hood [Re: poisoned]
    #26624267 - 04/24/20 09:36 AM (3 years, 9 months ago)

I've been using bags. Why inflate them?
I should know at this point I guess.
I have a FH so what just fill them with air?


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Offlinepoisoned
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Re: Inflating spawn bags without a flow hood [Re: Hobbit GDF]
    #26624782 - 04/24/20 01:28 PM (3 years, 9 months ago)

Inflate them because they need some oxygen and they are basically vacuumed when you get them out of the PC.

If you have a flowhood, inflating them isn't an issue at all. Just turn them towards the flowhood and let that clean air in. Without a flowhood, it's much more of an issue.


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Re: Inflating spawn bags without a flow hood [Re: poisoned] * 1
    #26625156 - 04/24/20 04:38 PM (3 years, 9 months ago)

Fwiw every farm I've toured that does their own spawn colonizes their spawn bags without inflating them.


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Offlinepoisoned
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Re: Inflating spawn bags without a flow hood [Re: bodhisatta]
    #26626109 - 04/25/20 02:12 AM (3 years, 9 months ago)

How are they inoculated then?


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Re: Inflating spawn bags without a flow hood [Re: poisoned]
    #26626236 - 04/25/20 04:42 AM (3 years, 9 months ago)

Stabbed or g2g'd into then sealed and folded back up


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Re: Inflating spawn bags without a flow hood [Re: bodhisatta]
    #26626440 - 04/25/20 07:25 AM (3 years, 9 months ago)

Yeah, and when you open them for g2g, they receive some air. Because straight out of the PC, they're basically vacuumed. That's what I understand as inflating.


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Re: Inflating spawn bags without a flow hood [Re: poisoned] * 1
    #26626475 - 04/25/20 07:38 AM (3 years, 9 months ago)

I see lots of hobby growers hang bags or put them on shelves with the bags "inflated"

Ive seen farms just stack the blocks all nice with the filters facing outward. The bags looks like they had the air squeezed back out to make them stack well


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Re: Inflating spawn bags without a flow hood [Re: bodhisatta]
    #26626498 - 04/25/20 07:51 AM (3 years, 9 months ago)

That's interesting!


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Offlinetedoro
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Re: Inflating spawn bags without a flow hood [Re: bodhisatta]
    #26628057 - 04/25/20 07:54 PM (3 years, 9 months ago)

Quote:

bodhisatta said:
I see lots of hobby growers hang bags or put them on shelves with the bags "inflated"

Ive seen farms just stack the blocks all nice with the filters facing outward. The bags looks like they had the air squeezed back out to make them stack well




This makes me feel better. I hang and get a few quarts of air in there, but RR always spoke of gobbling up as much air in there as you can when open. (Mine are never open, I stab them)


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineBlue Helix
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Re: Inflating spawn bags without a flow hood [Re: Blue Helix]
    #26816233 - 07/10/20 10:53 PM (3 years, 7 months ago)

Just a follow up on this: after I thought about it, it just didn't seem practical.  I had to ask myself: is hanging the bags up and waiting really harder than poking another hole in them pumping them up and filling the hole in with hot glue?  Also looking at my bags I asked myself "Have I ever had a bag stall from lack of oxygen - EVER?"  The answer to both questions was no, so I never did this.  It was a nice idea, but I didn't think it made much sense for me.  I don't have gas exchange problems with the bags in the first place, so it was a solution to a non-problem.


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