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Offlinemrjim202000
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Registered: 02/20/04
Posts: 285
Last seen: 5 years, 9 months
WBS qusetion (another i know sorry)
    #2662598 - 05/11/04 10:09 AM (20 years, 8 months ago)

would it be safe to soak the seed for 12 - 24 hr to alow endospores to germinate, the strain and put into jars to be steamed for 1hr to kill the endospores and leave viable substrate to inc, with liquid culture ?? or am i setting up for failure with no PC (and no i cant buy one.. i have no place to put it and my GF complaines about all the crap i have now ... oh well)

thanks peace


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InvisibleNewfoundFreedom
Manure whore

Registered: 05/06/04
Posts: 1,915
Re: WBS qusetion (another i know sorry) [Re: mrjim202000]
    #2662614 - 05/11/04 10:12 AM (20 years, 8 months ago)

With grains, I'd say a PC is a necessity, however I have seen grains done without.

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Offlineluckytriple6
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Re: WBS qusetion (another i know sorry) [Re: mrjim202000]
    #2663981 - 05/11/04 04:00 PM (20 years, 8 months ago)

fractal sterilization?


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Offlinecharvo
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Registered: 03/09/03
Posts: 654
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Re: WBS qusetion (another i know sorry) [Re: mrjim202000]
    #2664114 - 05/11/04 04:29 PM (20 years, 8 months ago)

what can do is soak the grain for 24 hrs no less.steam boil for 1 hour then wait 24 hrs and steam boil it again for 1 hour.even then you might get contamination,but it does work IME.i wouldn't try cutting corners without a pc.


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