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Offlinejcm4620
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Registered: 05/26/19
Posts: 6,700
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Re: My First Grow Journal [Re: Sockadin]
    #26612258 - 04/19/20 07:45 AM (3 years, 9 months ago)

also get your terminology down. it is very important when describing things to others that you get this correct. it helps to prevent others from seeing it and picking up bad habits & passing on the bad information. you inoculate jars and let them colonize. once colonized its often called spawn. spawning  them is the physical act of mixing your colonized grain(spawn) to your bulk substrate. so on and so on also paul stammets is a hack and his books are loaded with bad information and outdated techniques i assumed thats what books you were showing. the best thing you can do is read thru trusted and proven teks on here. every question you could possibly have to ask has already been asked 1,000 times. the search bar in the upper right is the best tool this site has to offer. you can literally ask it a question like you were talking to someone and it will tell you what you want to know. once learned how to use it properly that is. also when i said shoot it with a shotgun that was obviously just an attempt at adding some color to the fact that the thing is a horrible idea and will give you so many problems you will eventually feel like shooting it with a shotgun😃 to start look up bods and pastys teks and go from there. good luck to ya


--------------------
PANAEOLUS FRUITING MADE SIMPLE



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OfflineRivendell
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Registered: 03/29/20
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Last seen: 1 year, 6 months
Re: My First Grow Journal [Re: jcm4620]
    #26612278 - 04/19/20 07:55 AM (3 years, 9 months ago)

Appreciate the feedback.. Definitely a noob cultivator, but have put some effort into research prior to starting all this.  Here through the 100s of forums and read/reread a bunch of the posted teks.  By no means hating on the community knowledge.  Trying to suck it up like a sponge.  Right now is literally the first experiment and trying to get a setup going that can do trays (vs small shoeboxes) and straw bags (have the lime/straw/container to sterlize on the way from Amazon)  for my oysters, etc.  As for Bods/Pastys.. literally had the up from the beginning and have plans to follow for the agar pour tek and the bucket coir tek.  Can you elaborate some more on the Stamets book issue?  I'd heard a lot of good things and am using it as a manual for the different life cycles/stages to understand more as I grow into this hobby.
Any specifics that I should look out for?  Sorry I missed the playfulness..seems some want to see failure.  I'm ok with failure, because its the only way you learn (as long as one actually learns the lessons from the failures).  Which is why I'm posting a journal vs just doing it on my own and not just lurking.


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Offlinebigmanmcbig
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Registered: 02/09/19
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Last seen: 3 years, 9 days
Re: My First Grow Journal [Re: Rivendell]
    #26612348 - 04/19/20 08:19 AM (3 years, 9 months ago)

Don't let people get you down my man. I think what you have is amazing and you will have a lot of fun like I did messing around and having successes. You're good sir.


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Offlineredhandmat
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Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: My First Grow Journal [Re: bigmanmcbig]
    #26612372 - 04/19/20 08:28 AM (3 years, 9 months ago)

Quote:

bigmanmcbig said:
Don't let people get you down my man. I think what you have is amazing and you will have a lot of fun like I did messing around and having successes. You're good sir.




I (at least) was not tryign to get him down. Im new myself and wasnt sure if I had missed some things. So I asked to see if what he was doing was necessary. He now made it clear that he is just playing with it and trying some things out like his SAB FH mix. And thats cool! This is a hobby, and we do it because we find it fun. So the more fun you have the better :laugh: (just maybe make it clear as to not confuse people)


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OfflineRivendell
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Re: My First Grow Journal [Re: redhandmat]
    #26612397 - 04/19/20 08:37 AM (3 years, 9 months ago)

Nah.. you guys aren't getting me down. I've seen some of the vinegar on the FB groups and know what to expect.  Also just setting expectations with those that aren't fans of my setup.. that this is my very first attempt at mushie cultivation and I'm still in the process of getting things pulled together.  Just a virgin breaking his cherry.  I get that I have a ton to learn and I do appreciate the feedback.

So what should I look out for in the Stamets as outdated knowledge?  I do like the charts, etc they provide for tracking.. as that's the science of it all.  Also learning more about the lifecycle & stages.. troubleshooting section and per species grow info is useful.  (I'm assuming that is whats off?)  Right now I'm a sponge and don't even have the language down right..lol


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Offlineredhandmat
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Re: My First Grow Journal [Re: Rivendell]
    #26612434 - 04/19/20 08:48 AM (3 years, 9 months ago)

Quote:

Rivendell said:
Nah.. you guys aren't getting me down. I've seen some of the vinegar on the FB groups and know what to expect.  Also just setting expectations with those that aren't fans of my setup.. that this is my very first attempt at mushie cultivation and I'm still in the process of getting things pulled together.  Just a virgin breaking his cherry.  I get that I have a ton to learn and I do appreciate the feedback.

So what should I look out for in the Stamets as outdated knowledge?  I do like the charts, etc they provide for tracking.. as that's the science of it all.  Also learning more about the lifecycle & stages.. troubleshooting section and per species grow info is useful.  (I'm assuming that is whats off?)  Right now I'm a sponge and don't even have the language down right..lol




Im an academic (not in mycology but in sociology and business studies) so I respect wanting to follow the science behind it and seeking to optimize parameters (such as temp, humidity, etc) and make efficient use of all resources. But I have learned that when it comes to practical things, such as actually growing plants or mushrooms, you should start by keeping it as simple as possible and getting attention to the basics: so actually growing mushrooms by going print/syringe/tissue to agar plates, cleaning agar plates, then to grain and spawning to simple tecs like this SFF tek . And then growing mushrooms, choosing clones, isolating good strains etc.

Much of this is contingent on not making it harder than it should. As an example that SFF tek I linked above is done so its a do-it-and-forget-it tek that makes efficient use of your time and space. IMO thats especially important for when you want to some day go to commercial as you have said. And once you have a few growths on your belt you can make use of science books to really dial in and optimize even more parameters. But at least then you can judge what it working for you and what is not working. By now we know that a good PC and Flow Hood is something that you can make use of for a long while.


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OfflineRivendell
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Re: My First Grow Journal [Re: redhandmat] * 1
    #26612545 - 04/19/20 09:33 AM (3 years, 9 months ago)

Here are my materials for the next steps I'm planning on jumping into.

PC


My agar and mixing flask


Have a collection of petri dishes
plastic


borosicilate glass - 100mm/60mm


Coir Bucket tek/pellets for bags


Getting some materials for straw bags/cold lime pasteurization that haven't been delivered yet.. Maybe some other things I'm not thinking of.. but as I'm reading the preferred teks of each stage I'm trying to emulate it.


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Offlinebigmanmcbig
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Registered: 02/09/19
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Last seen: 3 years, 9 days
Re: My First Grow Journal [Re: Rivendell] * 2
    #26612827 - 04/19/20 11:55 AM (3 years, 9 months ago)

Sweet stuff my man. go crazy


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Offlineredhandmat
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Registered: 05/09/19
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Last seen: 2 years, 8 months
Re: My First Grow Journal [Re: bigmanmcbig] * 1
    #26612849 - 04/19/20 12:03 PM (3 years, 9 months ago)

looks awesome!


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OfflineLogicaL ChaosM
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26612850 - 04/19/20 12:04 PM (3 years, 9 months ago)

That's some Top-Shelf equipment and supplies u got there! We aint fuckin around! :eek:


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OfflineRivendell
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Re: My First Grow Journal [Re: LogicaL Chaos] * 1
    #26612932 - 04/19/20 12:32 PM (3 years, 9 months ago)

lol.. thanks, not trying to anyways ;-)

If I'm getting into it.. might as well dive in the deep end.


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OfflineLogicaL ChaosM
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26612936 - 04/19/20 12:35 PM (3 years, 9 months ago)

Might as well get the good stuff, aimrite?

:popcorn:


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26612942 - 04/19/20 12:38 PM (3 years, 9 months ago)

Have the temp dialed in and stable now at 83.3.  Humidity seems to be an issue keeping up though (although it was at 93% @ the lower temp prior to intro of the heater unit). 


Might have too much water in the lower chamber (although it does seem to be churning out fog and filling the lower resevoir)...

Can see the fogger bubbling and the chamber humid


or the rise in heat might be sapping the humidity, which I noticed as I was getting the temp boosted up.. it was dropping at a corresponding level.  (seen here at 86% earlier on)


I don't see or feel any leaks on the PVC pipes.. hmm..



Also live in the SW US so humidity is an issue inherently in the environment.


Edited by Rivendell (04/19/20 01:00 PM)


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26612944 - 04/19/20 12:39 PM (3 years, 9 months ago)

Exactly.  While I understand some choose the BRF/PF Tek methods to start and learn.. I know I have to get here with some of this equipment/tek anyways.. so why not cannonball in.. make a few hits and misses.. learn.. then eventually churn out canopies.

See you have 56k posts Logical.. if you have thoughts along the way, I'm here to learn, improve & work on perfecting techniques and BE.


Edited by Rivendell (04/19/20 01:02 PM)


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26616635 - 04/21/20 12:10 AM (3 years, 9 months ago)

Day 3 - Not much seen on the existing grain.  Gave it a little shake, but not much else to report there.. so onto the main event.. Today was agar day! 

Finally got around to reading up/vids on YT for proper operation and conditioning of the All American, so I thought I'd give it a test run and make some agar along the way.

First step was to Prep the All American 921 Pressure Canner.  Gave it a nice soap and water bath followed by lubricating up the seal.  Used vaseline per the Kneady Homesteader on YT.. seems she uses AA PC a lot for canning so followed her prep tek





Marked a kabob stick at 2" and 3" for reference markers.


Filled up the PC to approx 2.75" of water


Next up.. mix and cook up some agar.  Used BODs agar tek https://www.shroomery.org/forums/showflat.php/Number/21922023 with a PDA recipe https://www.shroomery.org/forums/showflat.php/Number/4308913.&nbsp;

- 400 ml water
- 10g agar agar
- 5g potato flakes
- 10ml honey
- 6 drops red dye







Microwaved to boiling point per tek and transferred to PC.. (with rubber flask cap in foil ball to sterilize when used to top in SAB)



Closed the PC up and checked gaps around..




Time to light up the stove!




Finally! Now we wait for the PC to get up to pressure... Happy 4/20/20 everyone!



Pressure is almost there.. Stabilizing at 250/15 psi..



Now we PC for 15 min @ 15 psi


Everything seemed to go ok.. so lets cut off the stove and let her cool to 0 psi



Cracked the PC open and looks like everything survived



Its cooled a little and with the assistance of a hand towel, I take the agar downstairs and place it into the SAB.  Turn on positive pressure fans pushing HEPA filtered air into the SAB and let environment settle (I know there are opinions on this.. I'm going to run with it until I see reason not to on agar) 



Where a new sleeve of plastic petri dishes await.  Also have a scalpel to cut the sleeve and an iso 70% soaked paper towel to wipe everything down.



I wipe down everything with the iso 70% include the petri dishes freshly removed from sleeve and start to pour stacks.  Its my first time so I have to work on the depth consistency.. but I have a bunch of plates prepped



Going to let them sit in there over night and cool/gel.. Have the UV light on so that'll do its magic.. and we'll see what things look like tomorrow!

Phew!


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26620894 - 04/22/20 07:41 PM (3 years, 9 months ago)

Quick update -

Inoculated some MS to agar yesterday.  1 gourmet - the Black Poplar/Pioppino and 2 cubes - Golden Teacher & B+. 

Made sure to get 4 or 5 drops per plate.. probably excessive, but I did see a lot of spores on the plates which made me hopeful that I don't have too high a contam/fail rate.  Used a sterilized (flame) loop to zigzag solution across agar.



Seems to be quite a bit of condensation on the top... is this more of a plastic PP5 vs Borosilicate glass issue?




I'm sure mostly due that I didn't let it cool enough.  I'll know more for the next pour, but something to keep an eye on.  Still a few more gourmets and cubes to put to plate.

Looks like I'll have 1 less plate for the next round of transfers though..


How long does it take for spores to start developing on agar?  I'm seeing some things saying 8 days to 2 weeks, so more curious as this is the first attempt.  Also see a few threads suggesting maybe the sterilized loop wasn't the way to go?

Still plenty to learn.. if you have any suggestions or tips, feel free to drop a note (be nice.. literally first time for all of this).


Edited by Rivendell (04/22/20 07:51 PM)


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26625560 - 04/24/20 07:48 PM (3 years, 9 months ago)

Trying to regulate the environment better, while allowing for better airflow locally to the boxes.. so enter the indoor greenhouse (EZ-UP)



Have it set back a little from wall, we'll see how it regulates the heat... but have removed the painters plastic around the SAB/Fruiting Chamber.


Hopefully being able to pull back the heater will allow the humidity to come back up... but also have a second fogger coming tomorrow to double the HP on the humidity.



Front and rear doors to the greenhouse.. have the back closed up and folded under to keep an air flow seal


Windows on the sides.. we'll see how the air flow goes.. keeping the one window closed to keep heat in better


the exterior basement windows don't have best seals.. and there is a crawl space as well.. so we'll see if the one window is enough.. if not I'll adjust until the environment is stable and regulated better.


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OfflineSockadin
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Re: My First Grow Journal [Re: Rivendell]
    #26625590 - 04/24/20 07:58 PM (3 years, 9 months ago)

Very cool set up. But your overthinking the whole thing.

Oh but please do carry on cause now I'm totally sucked in to see how this turns out! It's almost as good as Tiger King.


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OfflineRivendell
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Re: My First Grow Journal [Re: Sockadin]
    #26625601 - 04/24/20 08:03 PM (3 years, 9 months ago)

Its really cold down there.. without the heater, temps are in the low 60s - insert the heater.

The humidity drops as the temp rises, so I'm going to see how adding another fogger effects the system. 

Still dialing in and playing with things.. at least gotta give me some love for the PDA agar for my first attempt ;-)


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OfflineSockadin
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Re: My First Grow Journal [Re: Rivendell]
    #26625610 - 04/24/20 08:06 PM (3 years, 9 months ago)

Haha just have fun with it man. I said my thing about how you don't need 1/2 of the shit you bought to grow. But I'm intrigued. Also I'm not like a "right way to be" guy.

So if you want to row your boat in your own direction. Dude it's your boat.

We are here to help if you have any questions. Carry on! This is my new favorite thread btw.


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