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OfflineShroomer4000
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Agar
    #26625115 - 04/24/20 04:17 PM (3 years, 9 months ago)

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Hopefully Inoculated my first agar dish successfully,I used a spore syringe following all the typical sterile procedures. Its been a couple of days and I notice dots on the dish mostly where  I put the spore syringe solution, could it be successful or to early to tell.


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InvisibleSmartattack
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Re: Agar [Re: Shroomer4000]
    #26625159 - 04/24/20 04:40 PM (3 years, 9 months ago)

If those dots you speak of begin to cloud up and grow you know you got bacteria. First sighn of myc will be a hazy spot radiating out from center and turning white soon after.


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OfflineThrill
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Re: Agar [Re: Smartattack]
    #26625164 - 04/24/20 04:44 PM (3 years, 9 months ago)

Those dots look like theyre sitting on the surface rather than a part of the surface. Did you squirt the spore solution in there like that? Or did you spread it around someway?


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OfflineShroomer4000
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Re: Agar [Re: Thrill]
    #26625172 - 04/24/20 04:48 PM (3 years, 9 months ago)

I think I used to much and thats why its on the surface.


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OfflineThrill
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Re: Agar [Re: Shroomer4000]
    #26625439 - 04/24/20 06:52 PM (3 years, 9 months ago)

Just wait n see what happens with it. If the spots grow and get cloudy yellowish white, itll be bacterial. Its common on germ plates, especially one where liquid is involved.

I just happen to have a nasty bacterial section in one of my plates right now, so Ill drop the pic so you can see bacterial vs mycelium on a germination plate. Youll be able to tell the bacterial section lol.



Edit: oh, and with a syringe, usually youd wanna put 1 or maybe 2 drops at most on the plate. You dont want a lot of standing water, at least I dont. I usually put a drop or 2 in the middle, then streak it around in various overlapping patterns.


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OfflineCocaineBuffet
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Re: Agar [Re: Thrill]
    #26625494 - 04/24/20 07:23 PM (3 years, 9 months ago)

I would suggest doing some more plates in the meantime. Just 1 drop is plenty.


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