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OfflineSentinelFungi
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Registered: 04/05/20
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What happened? First attempt colonization failure * 1
    #26624715 - 04/24/20 12:59 PM (3 years, 9 months ago)

Hi guys.

I tried PF tek with BRF cakes as per RR. Had some issues with my jars sucking up more spore syringe due to me inadvertently creating little vacuums with my lids (I lids shown below, I was getting no growth after a few days and replaced the tyvek with micropore tape to cover the holes in the lid - I think the adhesive I used to attach the tyvek inadvertently sealed the lid). The jars were very loosely packed and top edges were cleaned/dried well.

So over 8 250ml jars, I used 20ml of spores via syringes. 2 weeks later, 2 show visible colonization with one contaminated (and turfed) while the other is stuck at about 5% since then. All other containers show absolutely nothing. The temp in the room is about 25C, on a shelf with light exposure. I'm not sure what the problem is.

My one thought is that the way RR does the cake jars on his video, the mixture seems to be on the dry side, meaning if you grab a handful and squeeze it, you will get no drips of water coming out of it (using his proportions for 5 jars of 1 cup BRF, 1 cup water, 2 cups vermiculite).

My other thought is maybe there weren't very many spores in the syringe. Certainly they weren't visible in either syringe (1 of Golden Teacher, 1 of Blue Meanie), and I did shake well.

Any thoughts or ideas? I'm super disappointed by this result obviously but am just wanting to figure it out for next time. Is there any way of attempting to gain anything from the jars that haven't shown any growth/colonization? Should I add some sterile water? Should I try adding more spores?

Any advice would be very much appreciated.

Thanks!

SF



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OfflineSentinelFungi
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Re: What happened? First attempt colonization failure [Re: SentinelFungi]
    #26627116 - 04/25/20 12:59 PM (3 years, 8 months ago)

Hi guys. Bumping. Really hoping some one can give me some direction as to what went wrong. Bought all this gear and hoping to be able to use the knowledge of this forum for it instead of solo trial and error.

I did take the micropore tape off the lids and put over a filter paper (held down with the lid rim scrowed on).


Edited by SentinelFungi (04/25/20 01:04 PM)


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OfflineLogicaL ChaosM
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Re: What happened? First attempt colonization failure [Re: SentinelFungi]
    #26627151 - 04/25/20 01:09 PM (3 years, 8 months ago)

I would advise putting your jars near a warm heat source. It might help. Give a couple more weeks then call it a fail grow. It sounds like the spore syringe didn't have enough spores in it.


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InvisibleRoger Clemency
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Re: What happened? First attempt colonization failure [Re: LogicaL Chaos] * 1
    #26631714 - 04/27/20 08:50 AM (3 years, 8 months ago)

You should have 4 holes around the lid, and nothing covering those holes. When you inoculate you want to put the tip of the needle against the glass so the spore solution runs down and spreads out a bit.

I don’t know if these would’ve helped your current situation but they’re good to know if you restart.


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends


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OfflinePinkStormtrooper
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Re: What happened? First attempt colonization failure [Re: Roger Clemency] * 1
    #26631785 - 04/27/20 09:27 AM (3 years, 8 months ago)

dont give up now. get another juicer tube and try again.

but IMO start totally over fresh. I think you were screwed before you started because of the vaccuum issue you experienced, I dont think it prepped right.

fix your lids up and do it again and you will have better luck.


--------------------
"say, you got a little astroglide on your moustache"


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OfflineSentinelFungi
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Re: What happened? First attempt colonization failure [Re: Roger Clemency]
    #26637037 - 04/29/20 03:13 PM (3 years, 8 months ago)

Thanks for the tip! I actually took off the micropore tape and covered it with a paper filter to keep any contaminants out.

The thing with leaving the holes open to air is I wonder if you'll get increased contaminants. Otherwise what would be the point of using a SAB? If contaminants from the air could land on the needle and get in the jars during inoculation, certainly they are small enough to get through the needle holes, no?

I started a new batch about 4 days ago making sure the verm-BRF was wet enough with the sqeeuze test and the jars have started to colonise so I think moisture was 1 issue. The second was not having more air allowed inside I think since the old jars started improving after opening the. Needle holes and covering the lids with paper filters.

Fingers crossed!


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OfflineA.k.aM
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Re: What happened? First attempt colonization failure [Re: SentinelFungi] * 1
    #26637148 - 04/29/20 04:19 PM (3 years, 8 months ago)

Exposing uncolonized spawn or cakes to air will for sure contaminate them.

SAB works because there’s no air currents to pick up contams and help them travel to your stuff.

They all just fall to the ground and then you go to work.


--------------------
LAGM2020


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InvisibleRoger Clemency
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Re: What happened? First attempt colonization failure [Re: A.k.a] * 1
    #26637308 - 04/29/20 05:37 PM (3 years, 8 months ago)

The dry vermiculite layer up top is what stops contaminates from getting to the cake.


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends


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OfflineSentinelFungi
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Re: What happened? First attempt colonization failure [Re: Roger Clemency]
    #26638314 - 04/30/20 05:50 AM (3 years, 8 months ago)

Thanks for the responses. I used a SAB. Issues from the first batch were multiple but I think I've finally got the basics. The first batch were accidentally made into mini vacuums so no air, they too little water, too little air even after I released the vacuum seals (still had the holes covered with micropore tape), and the dry verm layer is also scant.

I understand the contams issue now moving forward so thank for all the great advice! 🙏🏽

I'll post some pics on e the colonization is closer to complete.

Thanks again!


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OfflineSentinelFungi
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Re: What happened? First attempt colonization failure [Re: SentinelFungi]
    #26678020 - 05/18/20 01:17 AM (3 years, 8 months ago)

Hello all. The jars seemed to have been reinvigorated by airflow and an injection of water into each. There is 1 jar that stalled (not sure what to do about it -- seems to be sitting at 40% colonization), then the next guy looks suspicious for contams. I had a jar earlier on that showed green mold so I tossed it. But the one here shows some brown gunk but looks like well colonized stuff below. I'm guessing I should just chuck it out, but wanted to see if there is some common brown gunk that isn't a nightmare...



Thanks again!


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