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OfflineJellatinous
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From monotub Mycelium to Agar?
    #26623507 - 04/23/20 09:44 PM (3 years, 9 months ago)

I'm new to using agar. I just made some new malt agar plates.

I have a monotub that's getting close to fruiting. The mycelium is growing on the top of the monotub.

My question is...

Can I cut a piece of mycelium off of my monotub and put it onto the agar (in my still air box)?

Any advice on this will be greatly appreciated.


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OfflineYeetusdeetus
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Re: From monotub Mycelium to Agar? [Re: Jellatinous]
    #26623556 - 04/23/20 10:12 PM (3 years, 9 months ago)

You could do that but you’d probably get better fruiting genetics by cloning.


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Invisiblegenesis128
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Re: From monotub Mycelium to Agar? [Re: Yeetusdeetus]
    #26623566 - 04/23/20 10:20 PM (3 years, 9 months ago)

I think it should work. But the substrate would most likely contam the plate. You could probably transfer helathy myc to a new plate though.

But best route is to get a piece of tissue from inside of a fruit, once you have some... Cause it's clean in there. If you don't get fruits it's probably not even worth it to clone that culture.


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OfflineYeetusdeetus
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Re: From monotub Mycelium to Agar? [Re: genesis128]
    #26623575 - 04/23/20 10:28 PM (3 years, 9 months ago)

The myc in the sub of a ms grow contain substantially more genetic variety than myc you’d get from a cloned fruit. That’s why you can have extremely potent fruit and duds in the same tub when you grow from a print or syringe instead of a clone or tested fruiting isolate.


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OfflineYeetusdeetus
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Re: From monotub Mycelium to Agar? [Re: Yeetusdeetus]
    #26623583 - 04/23/20 10:31 PM (3 years, 9 months ago)

If you do get a fruit that you want to clone it’s important that you tear it open, as cutting into it with a blade can transfer contaminants to the sterile portion of the fruit. Treat it like you would an open petri, no hands above sterile media etc.


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Invisiblec10h12n2o
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Re: From monotub Mycelium to Agar? [Re: Yeetusdeetus]
    #26623766 - 04/24/20 01:37 AM (3 years, 9 months ago)

Just clone a fruit, makes no sense to go from sub to agar, it's not sterile and has no advantage vs cloning


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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OfflineJellatinous
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Re: From monotub Mycelium to Agar? [Re: c10h12n2o]
    #26624259 - 04/24/20 09:32 AM (3 years, 9 months ago)

Gotcha. Good consensus. Thank you all.

I'l wait until this monotub fruits & then take the spores from the caps and transfer them to the agar.

Is there a "cleaner" ot best way to do that?


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OfflineJellatinous
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Re: From monotub Mycelium to Agar? [Re: Yeetusdeetus]
    #26624261 - 04/24/20 09:33 AM (3 years, 9 months ago)

I see, you answered my question. Thanks for the advice


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Offlineverum subsequentis
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Re: From monotub Mycelium to Agar? [Re: Jellatinous]
    #26624450 - 04/24/20 11:02 AM (3 years, 9 months ago)

What they all said. Wait for the healthiest, fattest, fastest, most clustering.... Fruit.


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Invisiblec10h12n2o
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Re: From monotub Mycelium to Agar? [Re: Jellatinous]
    #26624988 - 04/24/20 03:04 PM (3 years, 9 months ago)

Quote:

Jellatinous said:
Gotcha. Good consensus. Thank you all.

I'l wait until this monotub fruits & then take the spores from the caps and transfer them to the agar.

Is there a "cleaner" ot best way to do that?




No no, dont bother with the spores, clone the fruit. RIP open the stem and put some clean tissue on agar


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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OfflineJellatinous
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Re: From monotub Mycelium to Agar? [Re: c10h12n2o]
    #26626500 - 04/25/20 07:52 AM (3 years, 9 months ago)

Quote:

c10h12n2o said:
Quote:

Jellatinous said:
Gotcha. Good consensus. Thank you all.

I'l wait until this monotub fruits & then take the spores from the caps and transfer them to the agar.

Is there a "cleaner" ot best way to do that?




No no, dont bother with the spores, clone the fruit. RIP open the stem and put some clean tissue on agar




OK I see. Would it make the moat sense for me to take a small pin or just wait till it is finished fruiting and harvested?


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Offlineredhandmat
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Re: From monotub Mycelium to Agar? [Re: Jellatinous]
    #26626518 - 04/25/20 08:03 AM (3 years, 9 months ago)

Quote:

Jellatinous said:
I'm new to using agar. I just made some new malt agar plates.

I have a monotub that's getting close to fruiting. The mycelium is growing on the top of the monotub.

My question is...

Can I cut a piece of mycelium off of my monotub and put it onto the agar (in my still air box)?

Any advice on this will be greatly appreciated.




You really should just use the inside tissue of a mushroom in a cluster. Its much better and will give you some awesome genetics. Make sure to get a spore print once you shrooms grow too. Then you can work with both spores to agar and tissue/clone to agar.

And to answer your last question, pins also work, but I would recommend to wait a little longer.... But you have nothing to lose really.


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Offlineverum subsequentis
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Re: From monotub Mycelium to Agar? [Re: Jellatinous]
    #26626522 - 04/25/20 08:04 AM (3 years, 9 months ago)

Pins a harder to get clean tissue from because they are so small. The outside of the fruit is covered in microscopic shit. That's why we  "rip open the fruit"  to get to the clean inner tissue. This must be done correctly to avoid contams.

Search for "taking clones" or "cloning" and see what you find. There's got to be a good write up. Or got to YouTube and watch Rogers "let's grow mushrooms" videos. He's got a part on cloning.


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OfflineJellatinous
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Re: From monotub Mycelium to Agar? [Re: verum subsequentis]
    #26626548 - 04/25/20 08:19 AM (3 years, 9 months ago)

Thanks guys. I really appreciate the wisdom.


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Offlineverum subsequentis
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Re: From monotub Mycelium to Agar? [Re: Jellatinous]
    #26626603 - 04/25/20 08:52 AM (3 years, 9 months ago)

Yup

https://www.shroomery.org/forums/showflat.php/Number/17784996

I didn't read through this but Frank knows his shit.


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