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OfflineIzzamagic
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Casing before sterilization for invitro
    #26621716 - 04/23/20 07:47 AM (4 years, 8 months ago)

So from what I have read it seems to be the consensus that casing layers primarily serve to create a somewhat even humid micro-climate as well as low nutrient density site for pins.

When fruiting off grains invitro in ziplocs as in the PODs tek, would it make sense to case prior to sterilization, and perhaps innoc with LC through a injection port low on the ziploc?

It seems if there is not something I am missing that this would produce better results than just grain and would remain a "one-step" process basically.

Please let me know your thoughts on other functions of casing that may be adversely affected by this method.

Thanks!

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OfflineIzzamagic
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Re: Casing before sterilization for invitro [Re: Izzamagic]
    #26636897 - 04/29/20 01:47 PM (4 years, 8 months ago)

Would this question better be addressed in advanced mycology?

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Offlinepoisoned
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Re: Casing before sterilization for invitro [Re: Izzamagic]
    #26636908 - 04/29/20 01:53 PM (4 years, 8 months ago)

If you want to do casing, you should do it after it's fully colonized and grown. Casing is supposed to be non-nutritious and is only there to provide microclimate.

If you're dealing with grains, the best way to increase yield would be to spawn your grain to bulk substrate. Maybe there's some tek for invitro bulk substrate?

And no, that's not advanced mycology.


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OfflineIzzamagic
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Re: Casing before sterilization for invitro [Re: poisoned]
    #26641401 - 05/01/20 01:55 PM (4 years, 8 months ago)

The reason I am interested in casing before sterilization is to maintain sterility and decent fruiting potential while keeping it a one step process as well. Perhaps this would be more useful for selection of ideal cultures under sterile conditions, similar to violets grain petri culturing tek.

Edited by Izzamagic (05/01/20 02:13 PM)

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Offlinepoisoned
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Re: Casing before sterilization for invitro [Re: Izzamagic]
    #26642570 - 05/02/20 01:28 AM (4 years, 8 months ago)

You don't need to maintain sterility once your grain is fully coloized


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Offlinemushpunx
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Re: Casing before sterilization for invitro [Re: Izzamagic]
    #26642716 - 05/02/20 03:10 AM (4 years, 8 months ago)

Quote:

Izzamagic said:
Would this question better be addressed in advanced mycology?




Definitely not


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Offlinemushpunx
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Re: Casing before sterilization for invitro [Re: mushpunx]
    #26642729 - 05/02/20 03:23 AM (4 years, 8 months ago)

First off how are you making your LC? If you aren't inoculating it with a clean agar culture you should not be using LC.


When most of us grow with grains, we take our colonized grains and spawn them to a bulk substrate like coir.
We do this in open air, and don't use casing layers.

Like Poisoned said, once your grain spawn is fully colonized, there is no need to maintain sterility.

If you are working with grains, the first step should always be obtaining a clean culture on agar. Spore syringes are never 100% clean. BRF cakes are quite forgiving, so they usually work fine for PF Tek, but if you want clean grain spawn inoculating with spore solution is not advised.

I would highly suggest you spawn to a bulk substrate instead of fruiting off grains.
It's incredibly simple... Colonize grain spawn, mix together with coir, fruit..


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Offlinemetaphoric
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Re: Casing before sterilization for invitro [Re: mushpunx]
    #26642749 - 05/02/20 03:48 AM (4 years, 8 months ago)

To answer your question yes, you could do that. The biggest downside I see is you won't be able to shake it so it'll take longer.

As others have said though, you are better off with other methods.


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Offlinemetaphoric
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Re: Casing before sterilization for invitro [Re: metaphoric]
    #26642750 - 05/02/20 03:50 AM (4 years, 8 months ago)

If your LC is clean and you're looking for a 1 step set-and-forget grow, look into bottle grows.


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OfflineIzzamagic
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Re: Casing before sterilization for invitro [Re: mushpunx]
    #26650787 - 05/05/20 01:31 PM (4 years, 8 months ago)

I know how to work with agar and make some MFN monotubs you brickheads. Im trying it :P

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InvisiblefahtsterM
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Re: Casing before sterilization for invitro [Re: Izzamagic] * 1
    #26650858 - 05/05/20 01:53 PM (4 years, 8 months ago)

You can do it.. I’ve done it on a really small scale with peu in 1/2 pints with brf.. just left a little bit of casing off of 2 sides so it didn’t touch the glass and inoced through a SHIP. 
 

I was just fucking around and seeing if I could get some pins to grow on the top invitro that I could quickly put to grain because they grew in a sterile environment.

It worked, but I was just testing it with a clone culture to see if I could get pins (if I were to to use the method, it’d be with a spore solution for PE) so I didn’t need to actually use the pin and fruited in a FC


So it can be done but like the other brickheads said, you don’t need to go through all that to get a cased bag to fruit.. just pasteurize some casing material and apply after its colonized

Faht

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OfflineIzzamagic
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Re: Casing before sterilization for invitro [Re: fahtster]
    #26651047 - 05/05/20 03:22 PM (4 years, 8 months ago)

Clean pins for the win. I shot up some pp5 pints with a spore syringe yesterday. Cheers

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Offlinepoisoned
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Re: Casing before sterilization for invitro [Re: fahtster]
    #26651172 - 05/05/20 04:27 PM (4 years, 8 months ago)

faht, how long did it take from inoculation to pinning?


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Re: Casing before sterilization for invitro [Re: poisoned]
    #26651223 - 05/05/20 04:51 PM (4 years, 8 months ago)

It took a really long time because of the peu culture I used is sooooo slow so this isn’t a good gauge for what normal cultures would do.. I think it was about a month. The casing was completely colonized right when the rest of the cake was so if it was a regular acting culture, it probably would have been around 3 weeks or earlier.. the parts of the casing that were colonized first did pin first. 

Faht

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