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Invisiblebrindle foxx
Doing it my way
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Registered: 11/11/18
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PC’d 10 petri dishes for 60 minutes @ 15 psi * 1
    #26621425 - 04/23/20 03:24 AM (3 years, 9 months ago)

Boy was that a great learning experience :lol:  tried to cut corners and do a no pour but it ended up melting together into a ball


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Offlinepoisoned
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Registered: 04/17/13
Posts: 1,738
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: brindle foxx]
    #26621451 - 04/23/20 03:44 AM (3 years, 9 months ago)

Buy some glass dishes and repeat.


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How I do glass dishes


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OfflineGrowtech
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Registered: 11/09/19
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: poisoned]
    #26621713 - 04/23/20 07:45 AM (3 years, 9 months ago)

Better yet, pour them.  Trying to pour before PC is going to be some boil over messiness in all probability.


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Mycolo.gyGenetics & yield tracking system


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Offlinepoisoned
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Registered: 04/17/13
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: Growtech]
    #26621725 - 04/23/20 07:50 AM (3 years, 9 months ago)

I never got any issues with glass boilover and many people here share my experience. I don't see any benefits to pouring glass dishes after the PC cycle.


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How I do glass dishes


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Invisiblebrindle foxx
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Registered: 11/11/18
Posts: 420 420 Posts!
Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: Growtech]
    #26621792 - 04/23/20 08:24 AM (3 years, 9 months ago)

Quote:

Growtech said:
Better yet, pour them.  Trying to pour before PC is going to be some boil over messiness in all probability.



Guess I didn’t see any info of anyone advising against the PC of petris. Done it just fine with PP5 cups. Although i had a PP5 container in the PC and it got warped. Guess Sticking with pyrex is overall better.


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OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: poisoned]
    #26621795 - 04/23/20 08:25 AM (3 years, 9 months ago)

POOOOOUUURRRR!!!!

:fuckyeah:  :fuckyeahdance:  :fuckyeah:  :fuckyeahdance:

Just get over the learning curve and it will allow you to take your agar game to the next level!

You can use both a SAB and a flowhood so even posible on the cheap side of life :naughty:


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:awecid:


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OfflineThe Mycologist
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Registered: 05/06/16
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: poisoned]
    #26621796 - 04/23/20 08:26 AM (3 years, 9 months ago)

Quote:

poisoned said:
Buy some glass dishes and repeat.



:whathesaid:

I love my glass petris. As long as you let the pc cool down naturally you shouldn't have any issues


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Offlinepoisoned
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: Madoxx]
    #26621860 - 04/23/20 08:58 AM (3 years, 9 months ago)

Quote:

Madoxx said:
POOOOOUUURRRR!!!!

:fuckyeah:  :fuckyeahdance:  :fuckyeah:  :fuckyeahdance:

Just get over the learning curve and it will allow you to take your agar game to the next level!

You can use both a SAB and a flowhood so even posible on the cheap side of life :naughty:




What's next level about pouring vs no-pouring if you use glass? I've tried both ways and no-pour is just so superior in many ways to pouring. No watching of bottle to get to the right temperature, sterility guaranteed, no clumping when the bottom cools down, ...


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How I do glass dishes


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InvisibleLadysKnight
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Registered: 10/09/15
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: The Mycologist] * 2
    #26621867 - 04/23/20 09:00 AM (3 years, 9 months ago)

Quote:

The Mycologist said:
Quote:

poisoned said:
Buy some glass dishes and repeat.



:whathesaid:

I love my glass petris. As long as you let the pc cool down naturally you shouldn't have any issues




:whathesaid:
Glass no-pours are sexy as hail, and Pyrex is nice but unnecessary.


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Invisiblebrindle foxx
Doing it my way
I'm a teapot User Gallery


Registered: 11/11/18
Posts: 420 420 Posts!
Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: poisoned]
    #26621915 - 04/23/20 09:42 AM (3 years, 9 months ago)

Quote:

poisoned said:
Quote:

Madoxx said:
POOOOOUUURRRR!!!!

:fuckyeah:  :fuckyeahdance:  :fuckyeah:  :fuckyeahdance:

Just get over the learning curve and it will allow you to take your agar game to the next level!

You can use both a SAB and a flowhood so even posible on the cheap side of life :naughty:




What's next level about pouring vs no-pouring if you use glass? I've tried both ways and no-pour is just so superior in many ways to pouring. No watching of bottle to get to the right temperature, sterility guaranteed, no clumping when the bottom cools down, ...




Clumping is my biggest issue because im usually not babysitting the bottle before it solidifies... rather pour it ,pc it and forget it until I go pull it out to do agar work


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Invisiblebrindle foxx
Doing it my way
I'm a teapot User Gallery


Registered: 11/11/18
Posts: 420 420 Posts!
Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: Madoxx]
    #26621916 - 04/23/20 09:42 AM (3 years, 9 months ago)

Quote:

Madoxx said:
POOOOOUUURRRR!!!!

:fuckyeah:  :fuckyeahdance:  :fuckyeah:  :fuckyeahdance:

Just get over the learning curve and it will allow you to take your agar game to the next level!

You can use both a SAB and a flowhood so even posible on the cheap side of life :naughty:



:hahathatsfunny:


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InvisibleKimble
Idiot
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Registered: 03/08/18
Posts: 508
Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: poisoned] * 1
    #26621939 - 04/23/20 09:54 AM (3 years, 9 months ago)

Quote:

poisoned said:
I never got any issues with glass boilover and many people here share my experience. I don't see any benefits to pouring glass dishes after the PC cycle.






After you put me onto it, i tried it and it works great.
Once i did it the first time i wondered what reason i would have to pour them after the pc cycle... I can't think of one.
I don't think I'll go back to pouring them in the Sab anytime soon. I'm familiar with both methods.
Also, i agree with lady, soda glass ones work suitably.


Edited by Kimble (04/23/20 10:33 AM)


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OfflineThe Mycologist
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Registered: 05/06/16
Posts: 3,024
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: Kimble]
    #26622022 - 04/23/20 10:41 AM (3 years, 9 months ago)

Hmm you wrap them for the pc cycle?


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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InvisibleKimble
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: The Mycologist]
    #26622024 - 04/23/20 10:42 AM (3 years, 9 months ago)

Sure do.


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Offlinepoisoned
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: Kimble]
    #26622052 - 04/23/20 10:59 AM (3 years, 9 months ago)

Yeah, that's what the stacks look like after the PC cycle:


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How I do glass dishes


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OfflineJustweed
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Registered: 04/01/20
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: Kimble]
    #26622055 - 04/23/20 11:00 AM (3 years, 9 months ago)

I did the same thing earlier this week! Next day went out and bought some tupperwares, boiled up some agar, poured into the little things, PC'd at 15psi for 45 minutes, threw some spores on, now waiting for them...Pushing 72 hours now and trying not to look until tomorrow, hopefully see something nice on em!


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Keep 'em high and tight guys....


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OfflineThe Mycologist
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: poisoned]
    #26622085 - 04/23/20 11:08 AM (3 years, 9 months ago)

Hmm Ive never tried wrapping.

Whats the motivation?


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Offlinepoisoned
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Registered: 04/17/13
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: The Mycologist]
    #26622098 - 04/23/20 11:12 AM (3 years, 9 months ago)

  • They stay nice and stable in your PC
  • They stay nice and stable in your cabinet
  • You don't need any additional packaging for storage
  • They won't open accidentally when you move them around.


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How I do glass dishes


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InvisibleFischer
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Registered: 06/03/10
Posts: 565
Loc: Downunder
Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: poisoned]
    #26622166 - 04/23/20 11:50 AM (3 years, 9 months ago)

Quote:

Kimble said:
Sure do.




What are you wrapping them with?  I'd assumed saran would melt.


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Offlinepoisoned
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Re: PC’d 10 petri dishes for 60 minutes @ 15 psi [Re: Fischer] * 2
    #26622168 - 04/23/20 11:53 AM (3 years, 9 months ago)

It's saran. It melts to some degree, which makes it really tight around the dishes. If you're loading multiple stacks, avoid having them touch or put some tin foil between them.


--------------------
How I do glass dishes


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