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OfflineMocha Magic Man
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Registered: 06/11/19
Posts: 4
Last seen: 2 years, 5 months
Hyphae interactions. cloning/isolation
    #26620669 - 04/22/20 05:33 PM (3 years, 9 months ago)

Greetings my esteemed fellow humans! Please forgive me if these questions have been asked/answered before. This is my first post and I am nervous that someone will point out some obvious post I have missed!! Anywho, I am currently trying to ensure potent, large yielding genetics (who isn't? ) by isolating some kickass genetics with agar. My main limiting factor here is petri dishes. I have made some reusable ghetto ones out of 1/2 pint jars (thank you PF tek) and have a sleeve of 20 sterile 100mm disposable plastic ones. So my main question is this: How can I best use my resources to find an optimal strain.. SO I have been doing my homework and I have not been able to figure out a few things. Essential I do not understand how Hyphae networks and different genetics integrate throughout a given substrate. So let's say I have a 5 qt jars of MS spawn mixed with coir and verm. Once the mycelium network has been established, to what degree do different parts have different genetics? I ask because I want to know the best way to take clones from a prolific flush and know which fruits will be best. Is it true that all fruits have varying genetics? And let's say I have a strain/variety that I want to keep WITHOUT isolating it further. How can I take an agar wedge (ultimately transfer to a master) without isolating? I am just getting started with the whole agar thing and have lot's of ideas and questions and I am a bit overwhelmed with my options. Can anyone point me in the direction of where to start/what to focus on? I have 6 qt jars currently colonizing with PE and I was going to hopefully get a nice clone or few from those. Meanwhile I have a PE spore syringe I want to try and isolate from. To be more concise: Can anyone point me to a resource/tell me about Hyphae interactions in terms of trading genetics and fruiting? Also I just started doing monotubs and don't know the best way to proceed. For getting a good clone with some magical genetics, is it a better idea to have multiple small minitubs or a couple medium sized or just one big monotub? I have 3 sizes available. I'll just leave it at that and see what comes of it! Any little bit helps and thanks in advance!!!
-MMM


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Offlinebrocepheushyde
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Registered: 04/16/20
Posts: 85
Last seen: 3 years, 4 months
Re: Hyphae interactions. cloning/isolation [Re: Mocha Magic Man]
    #26620678 - 04/22/20 05:37 PM (3 years, 9 months ago)

You're looking for rhizomatic mycelium growth when you're looking for mycelia to clone.



Not this stuff:


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: Hyphae interactions. cloning/isolation [Re: brocepheushyde]
    #26620756 - 04/22/20 06:15 PM (3 years, 9 months ago)


Here's a good example as well, I'm still isolating the rhizo but they look too beautiful not to share.


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Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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Offlinepoisoned
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Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: Hyphae interactions. cloning/isolation [Re: mushhead]
    #26621418 - 04/23/20 03:20 AM (3 years, 9 months ago)

Nothing wrong with tomentose growth. Actually, I find that first rhizo plate a bit suspicious.


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Invisiblewoofwoof
such mushrooms!
I'm a teapot User Gallery


Registered: 01/04/19
Posts: 1,127
Re: Hyphae interactions. cloning/isolation [Re: brocepheushyde] * 1
    #26621434 - 04/23/20 03:29 AM (3 years, 9 months ago)

Quote:

brocepheushyde said:
You're looking for rhizomatic mycelium growth when you're looking for mycelia to clone.



Not this stuff:




untrue. I have been fruiting Rustywhytes and they have been all tomatose. Had no issues with tomatose


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Invisiblemushhead
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I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: Hyphae interactions. cloning/isolation [Re: woofwoof]
    #26621943 - 04/23/20 09:55 AM (3 years, 9 months ago)

In my pics above the two plates were started at the same time, transferred from the same plate. The Rhizomorphic growth out grew the Tomentose by a long run, this is why I believe individuals don't really accept tomentose as good growth due to it being slow reaching.


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Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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Offlinepoisoned
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Registered: 04/17/13
Posts: 1,738
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Re: Hyphae interactions. cloning/isolation [Re: mushhead]
    #26621984 - 04/23/20 10:16 AM (3 years, 9 months ago)

The issue is that the same culture can switch from tomentose to rhizomorphic. I also try to make transfers from rhizo growth, but your post sounded almost like tomentose is bad. My early plates are usually all tomentose and we see noobs panicking around the boards when they get tomentose growth on their germination plates.


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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Hyphae interactions. cloning/isolation [Re: poisoned] * 1
    #26622007 - 04/23/20 10:31 AM (3 years, 9 months ago)

Quote:

poisoned said:
The issue is that the same culture can switch from tomentose to rhizomorphic. I also try to make transfers from rhizo growth, but your post sounded almost like tomentose is bad. My early plates are usually all tomentose and we see noobs panicking around the boards when they get tomentose growth on their germination plates.




This is important. Im a noob myself, and honestly if I didn't purposely ask if my MS growth was mycelium I would have tossed it because in the forums people love to just show off their rhizo plates, so new growers dont even know what early on MS myc even looks like.

But since we are on this topic. Is it actually possible to turn completely tomentose into rhizo just through agar transfers? Because my plates dont show any rhizo until they reach the glass wall and start climbing. At that point its possible to start seeing some individual "tentacles" or "threads" or wte they are called.


Edited by redhandmat (04/23/20 10:32 AM)


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 57 minutes, 51 seconds
Re: Hyphae interactions. cloning/isolation [Re: redhandmat]
    #26622028 - 04/23/20 10:43 AM (3 years, 9 months ago)

I battle with this all the time all my myce is tomentose :popcorn:


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Offlinepoisoned
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Registered: 04/17/13
Posts: 1,738
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Re: Hyphae interactions. cloning/isolation [Re: rumfor69]
    #26622071 - 04/23/20 11:04 AM (3 years, 9 months ago)

Try adjusting your nute contents. It seems more likely to go rhizomorphic if you lower the nutrition.


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Offlinerumfor69
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Re: Hyphae interactions. cloning/isolation [Re: poisoned]
    #26622102 - 04/23/20 11:16 AM (3 years, 9 months ago)

I use the just add water pda powder and I'm doing a ratio that would
use 30g of it to 1,000ml so just 0.45g of it in a 15ml 1/8" thick layer


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OfflineSockadin
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Registered: 01/03/10
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Last seen: 2 months, 20 days
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Re: Hyphae interactions. cloning/isolation [Re: rumfor69]
    #26622115 - 04/23/20 11:24 AM (3 years, 9 months ago)

Yeah back it down to 25g/1000ml and you will get the desired results.

I made some coffee agar for fun. But man it was way to nutrious. Thin wispy growth on all plates.


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Offlinerumfor69
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Registered: 08/05/11
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Re: Hyphae interactions. cloning/isolation [Re: rumfor69]
    #26623195 - 04/23/20 07:02 PM (3 years, 9 months ago)

Ok next dish I'll try that ratio. I really think my lack of aggressive
growth is affecting my work. Maybe not by a lot, but enough that I have
to find out.


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OfflineMocha Magic Man
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Registered: 06/11/19
Posts: 4
Last seen: 2 years, 5 months
Re: Hyphae interactions. cloning/isolation [Re: Mocha Magic Man]
    #26629580 - 04/26/20 11:23 AM (3 years, 8 months ago)

Well I hope the nutrition thing helps someone do better agar work!! Haven't really answered any of my questions though.. I have already bought RR's let's grow mushroom videos, and done a fair amount of research online so I understand stuff like tomentose growth versus rhizomorphic and hyphae and septa and all that jazz. Essentially I want to know where to best get clones and the logic behind it genetically. I probably only have enough agar to do a couple clones and isolations so I want to know as much as I can of what I am actually doing.
-MMM


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