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InfiniteConsciousn
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Registered: 04/21/20
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Why Did Only Some Buckets Fruit?
#26619026 - 04/21/20 11:57 PM (3 years, 9 months ago) |
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Hi all, thank you in anticipation.
My first time growing. Was given a spore print of an unknown active. I created agar plates and began the process of isolation. However rather than continue until I had a lot of isolated strains I decided to fruit some so I could clone the best.
No contam issues.
My successful flush came from a plate that was straight from the print. Other attempts were from plates after one more agar to agar transfer.
Successful flush had been grown on millet for about 5 weeks, the others on WBS using Doc's WBS tech for about 3 weeks.
All were in buckets with lids on in substrate for about 3 weeks before starting a 12/12 schedule.
I layered sub and myc with a top layer of sub. Just like Willy and let's grow mushrooms.
The sub and myc in all cases made a solid cake with great myc growth throughout.
75% cow poop (in a bag from garden store) 10% verm 10% coir 5% gypsum field capacity as per the above videos.
Successful flush: 12 gallon tote. Unsuccessful flushes: One was in a home depot bucket. 3 were in other buckets placed into a larger tote with fan/misting hole.
I didn't fan until going to fruiting conditions. Then I fanned and misted a few times per day.
I didn't create a micro climate with wax paper before going to 12/12 as suggested by willy as I didn't have any.
1 inch holes toward the top of the buckets. Small FAE holes just above the sub. Had painter's tape at first them micropore tape when went to fruiting 12/12.
It looks like the myc just stopped growing at some point in the unsuccessful buckets. At first I thought maybe it wouldn't produce then I noticed a ton of pins on the side. Wondering if it could have been moisture issues as the pins seemed to show in the micro climate and the unsuccessful buckets had lots of volume where the successful tote had sub/myc all along the bottom up to 3". Ie the unsuccessful buckets were all but one in a larger tote that was not full.
General humidity in this area is below 50%.
Should I give up on them or perhaps dunk them?
Successful tote
Great myc growth. Myc looked white and healthy. But no pins on top. However a ton of pins on the side.

This is the side of the bucket.
This is the pinning in the successful flush tote.
Home depot bucket. No pinning. Don't know if there's any on the side.
Is this CFL too bright? I got the one that was recommended on one of the message boards. But in this case it is close to the myc.

BTW Should I remove every one of these tiny pins before dunking? There are a gazillion.
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stevekaz28
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Registered: 04/21/20
Posts: 4
Loc: Colorado
Last seen: 3 years, 7 months
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If your humidity is under 50% I would blame it on that. The edge of the your mycelium cakes against the bucket are going to do a better job holding moisture longer simply because of a lack of air flow. It sounds to me like the top inch or 2 of your substrate in the bucket probably dried out further helping the trap the moisture further down in the bucket. I would assume that's why you see side pins and nothing on top. You need to keep your humidity above 90%....100% of the time. Also, the 2nd and third picture of the colonized substrate in the buckets look to me like you may have fruited them a bit early. While there is prominent mycelium growth poking out and along the bottoms and sides, I do see a large amount of bare substrate on the top. Perhaps those buckets could have gone another 4-5 days before fruiting. DISCLAIMER: Im pretty new to this art...take that with a grain of salt.
-------------------- "EVERYBODY LOVE EVERYBODY!!!!!!!" -Jackie Moon
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stevekaz28
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Registered: 04/21/20
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Loc: Colorado
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Re: Why Did Only Some Buckets Fruit? [Re: stevekaz28]
#26619043 - 04/22/20 12:14 AM (3 years, 9 months ago) |
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Also I would move that light a little further away and I would pull all those pins. Just because they're small doesn't mean they're not useful. If you dunk that cake with the pins they will just abort and become useless. Great little mushrooms for micro dosing. Several great medical benefits for the right person.
-------------------- "EVERYBODY LOVE EVERYBODY!!!!!!!" -Jackie Moon
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stevekaz28
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Registered: 04/21/20
Posts: 4
Loc: Colorado
Last seen: 3 years, 7 months
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Re: Why Did Only Some Buckets Fruit? [Re: stevekaz28]
#26619054 - 04/22/20 12:24 AM (3 years, 9 months ago) |
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Just saw your substrate recipe....You may want to cut down a bit on the manure and increase your coir a bit. Too much manure or worm castings will cause the substrate to be more dense. It will take longer for the mycelium to colonize and it will be near impossible to keep moisture consistent throughout. Using too much manure or castings can cause the bottom of the substrate to become muddy and over hydrated while you struggle to keep the top wet. Coir will help hold consistent moisture throughout your substrate while also providing excellent aeration properties. Id cut the manure down to 60% and bump up the coir that 15% you took from the manure. Also next time Ill read the entire post and answer everything at once.......dur
-------------------- "EVERYBODY LOVE EVERYBODY!!!!!!!" -Jackie Moon
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InfiniteConsciousn
Stranger

Registered: 04/21/20
Posts: 11
Last seen: 3 years, 8 months
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Re: Why Did Only Some Buckets Fruit? [Re: stevekaz28]
#26619112 - 04/22/20 01:30 AM (3 years, 9 months ago) |
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Quote:
stevekaz28 said: If your humidity is under 50% I would blame it on that. The edge of the your mycelium cakes against the bucket are going to do a better job holding moisture longer simply because of a lack of air flow. It sounds to me like the top inch or 2 of your substrate in the bucket probably dried out further helping the trap the moisture further down in the bucket. I would assume that's why you see side pins and nothing on top. You need to keep your humidity above 90%....100% of the time. Also, the 2nd and third picture of the colonized substrate in the buckets look to me like you may have fruited them a bit early. While there is prominent mycelium growth poking out and along the bottoms and sides, I do see a large amount of bare substrate on the top. Perhaps those buckets could have gone another 4-5 days before fruiting. DISCLAIMER: Im pretty new to this art...take that with a grain of salt.
Ok I'll keep a better eye on humidity this time around.
I think you're right, the top of the sub is pretty hard.
With those 2nd/3rd I decided to just go ahead as they seemed to stop and I was fruiting the others anyway.
Thanks
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InfiniteConsciousn
Stranger

Registered: 04/21/20
Posts: 11
Last seen: 3 years, 8 months
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Re: Why Did Only Some Buckets Fruit? [Re: stevekaz28]
#26619113 - 04/22/20 01:31 AM (3 years, 9 months ago) |
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Quote:
stevekaz28 said: Also I would move that light a little further away and I would pull all those pins. Just because they're small doesn't mean they're not useful. If you dunk that cake with the pins they will just abort and become useless. Great little mushrooms for micro dosing. Several great medical benefits for the right person.
The light is inside the tote so I can't move it further away. I can get a much less bright one though. The mushrooms don't use the light to grow do they? Just for direction?
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InfiniteConsciousn
Stranger

Registered: 04/21/20
Posts: 11
Last seen: 3 years, 8 months
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Re: Why Did Only Some Buckets Fruit? [Re: stevekaz28]
#26619114 - 04/22/20 01:33 AM (3 years, 9 months ago) |
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Quote:
stevekaz28 said: Just saw your substrate recipe....You may want to cut down a bit on the manure and increase your coir a bit. Too much manure or worm castings will cause the substrate to be more dense. It will take longer for the mycelium to colonize and it will be near impossible to keep moisture consistent throughout. Using too much manure or castings can cause the bottom of the substrate to become muddy and over hydrated while you struggle to keep the top wet. Coir will help hold consistent moisture throughout your substrate while also providing excellent aeration properties. Id cut the manure down to 60% and bump up the coir that 15% you took from the manure. Also next time Ill read the entire post and answer everything at once.......dur
Yeah it's pretty dense. This last lot I'm making up has closer to 20% verm and 20% coir, 5% gypsum and the rest in manure.
I'm going to put some foil over each tray that goes inside the tote this time as well.
Thanks
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