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Offlinetomcards
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How much MEA/liter of water?
    #26617762 - 04/21/20 01:10 PM (3 years, 9 months ago)

Guys,

I'm working with agar for the first time.  I used Bod's recipe of 50 grams of MEA in one liter of water. 

On 4/17, I streaked 5 year old spores on an agar plate.  On 4/20, I streaked fresh spores on another plate.  I'm waiting for signs of life.

Meanwhile, I've read a range of MEA/liter of water recommendations.  Some guys recommend 40 grams of MEA/liter.  Some recommend as little as 20 grams MEA/liter.  That's a huge range.

Please tell me how many grams of MEA/liter of water that you recommend for making agar plates that will be inoculated by spore streaking.  Is there a consensus?


Thanks.


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InvisibleSmartattack
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Re: How much MEA/liter of water? [Re: tomcards]
    #26617783 - 04/21/20 01:16 PM (3 years, 9 months ago)

I find 50 per liter great for growth, also easy to remember. I have varied my strength by a lot and it doesn't make a huge difference. Go lighter if you want to save money, but don't go heavy just cause you're rich. Myc grows stupid if its too strong.


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Offlinepoisoned
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Re: How much MEA/liter of water? [Re: Smartattack]
    #26618017 - 04/21/20 02:53 PM (3 years, 9 months ago)

That’s a whole bunch of LME. I’m usually way below 20g/l


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Invisiblenosf3r4tu

Registered: 03/26/19
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Re: How much MEA/liter of water? [Re: tomcards]
    #26618027 - 04/21/20 03:01 PM (3 years, 9 months ago)

For old spores you might want to lower the agar content of the recipe. Something like 1% works better than the regular 2% that is often used. That's because it's more soft and helps rehydrate the spores and still stiff enough to make a transfer.
As for MEA i don't know. Haven't used it yet.


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Offlinetomcards
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Re: How much MEA/liter of water? [Re: nosf3r4tu]
    #26618069 - 04/21/20 03:18 PM (3 years, 9 months ago)

Thanks for your input guys.  See what I mean about the wide range? 

Smartattack recommends 50g/liter. 

Poisoned uses 20g/liter.

There must be an optimal ratio.


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OfflineGrowtech
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Re: How much MEA/liter of water? [Re: tomcards]
    #26618147 - 04/21/20 03:56 PM (3 years, 9 months ago)

I also do 20g LME + 20g agar per liter of water.


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Offlinetomcards
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Re: How much MEA/liter of water? [Re: Growtech]
    #26618191 - 04/21/20 04:14 PM (3 years, 9 months ago)

So that's a total of 40g/liter.

To clarify, the MEA that I'm using is the pre-mixed, all-in-one, just-add-water type.


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InvisibleSmartattack
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Re: How much MEA/liter of water? [Re: tomcards]
    #26618201 - 04/21/20 04:17 PM (3 years, 9 months ago)

You guys are actually quite right. I do agar 2 liters at a time so 50g is my number. 50 in a liter does sound rich.


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Edited by Smartattack (04/21/20 04:17 PM)


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InvisibleSmartattack
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Re: How much MEA/liter of water? [Re: tomcards]
    #26618205 - 04/21/20 04:20 PM (3 years, 9 months ago)

Quote:

tomcards said:
So that's a total of 40g/liter.

To clarify, the MEA that I'm using is the pre-mixed, all-in-one, just-add-water type.




Im pretty sure typical recipes call for 10g per 500ml. Thats 20 of MEA not including agar per liter. i am a little on the rich side of the unmodded recipe at 25.


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Offlinetomcards
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Re: How much MEA/liter of water? [Re: Smartattack]
    #26618286 - 04/21/20 05:05 PM (3 years, 9 months ago)

Here's the MEA, purchased from a reputable dealer:






Their description says, "Premixed dehydrated agar formula. 25 grams of media are recommended per 500 ml of distilled water." 

That's 50 grams/liter, like I'm using, based upon Bod's tek. 

It sounds like I'm using the right amount.

I'm so confused.


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Offlinepoisoned
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Re: How much MEA/liter of water? [Re: tomcards]
    #26618297 - 04/21/20 05:11 PM (3 years, 9 months ago)

Bod's tek calls for 20g/L of MEA

But you have a premixed formula, so there's also agar inside. I'm not sure what the mixture is, but 50g/l of that sounds within reasonable amounts.


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InvisibleSmartattack
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Re: How much MEA/liter of water? [Re: poisoned]
    #26618310 - 04/21/20 05:19 PM (3 years, 9 months ago)

Yes, if it includes agar powder then it all makes sense. In a nutshell, get out of here now and just follow the directions.


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* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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Offlinetomcards
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Re: How much MEA/liter of water? [Re: Smartattack]
    #26618323 - 04/21/20 05:23 PM (3 years, 9 months ago)

Whew. Got it.

Thanks guys.


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Offlineshevanel
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Re: How much MEA/liter of water? [Re: tomcards]
    #26618350 - 04/21/20 05:35 PM (3 years, 9 months ago)

I use roughly 20-25 mea for 500ml

I use same exact premix.


Edited by shevanel (04/21/20 05:37 PM)


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Offlinetomcards
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Re: How much MEA/liter of water? [Re: shevanel] * 1
    #26618368 - 04/21/20 05:53 PM (3 years, 9 months ago)

Thanks for the reassurance Shevanel!


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