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tomcards



Registered: 04/24/15
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How much MEA/liter of water?
#26617762 - 04/21/20 01:10 PM (3 years, 9 months ago) |
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Guys,
I'm working with agar for the first time. I used Bod's recipe of 50 grams of MEA in one liter of water.
On 4/17, I streaked 5 year old spores on an agar plate. On 4/20, I streaked fresh spores on another plate. I'm waiting for signs of life.
Meanwhile, I've read a range of MEA/liter of water recommendations. Some guys recommend 40 grams of MEA/liter. Some recommend as little as 20 grams MEA/liter. That's a huge range.
Please tell me how many grams of MEA/liter of water that you recommend for making agar plates that will be inoculated by spore streaking. Is there a consensus?
Thanks.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,776
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Re: How much MEA/liter of water? [Re: tomcards]
#26617783 - 04/21/20 01:16 PM (3 years, 9 months ago) |
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I find 50 per liter great for growth, also easy to remember. I have varied my strength by a lot and it doesn't make a huge difference. Go lighter if you want to save money, but don't go heavy just cause you're rich. Myc grows stupid if its too strong.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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poisoned
untitled



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Re: How much MEA/liter of water? [Re: Smartattack]
#26618017 - 04/21/20 02:53 PM (3 years, 9 months ago) |
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That’s a whole bunch of LME. I’m usually way below 20g/l
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nosf3r4tu

Registered: 03/26/19
Posts: 775
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Re: How much MEA/liter of water? [Re: tomcards]
#26618027 - 04/21/20 03:01 PM (3 years, 9 months ago) |
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For old spores you might want to lower the agar content of the recipe. Something like 1% works better than the regular 2% that is often used. That's because it's more soft and helps rehydrate the spores and still stiff enough to make a transfer. As for MEA i don't know. Haven't used it yet.
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tomcards



Registered: 04/24/15
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Re: How much MEA/liter of water? [Re: nosf3r4tu]
#26618069 - 04/21/20 03:18 PM (3 years, 9 months ago) |
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Thanks for your input guys. See what I mean about the wide range?
Smartattack recommends 50g/liter.
Poisoned uses 20g/liter.
There must be an optimal ratio.
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Growtech
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Re: How much MEA/liter of water? [Re: tomcards]
#26618147 - 04/21/20 03:56 PM (3 years, 9 months ago) |
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I also do 20g LME + 20g agar per liter of water.
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tomcards



Registered: 04/24/15
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Re: How much MEA/liter of water? [Re: Growtech]
#26618191 - 04/21/20 04:14 PM (3 years, 9 months ago) |
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So that's a total of 40g/liter.
To clarify, the MEA that I'm using is the pre-mixed, all-in-one, just-add-water type.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,776
Loc: A thought
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Re: How much MEA/liter of water? [Re: tomcards]
#26618201 - 04/21/20 04:17 PM (3 years, 9 months ago) |
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You guys are actually quite right. I do agar 2 liters at a time so 50g is my number. 50 in a liter does sound rich.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
Edited by Smartattack (04/21/20 04:17 PM)
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,776
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Re: How much MEA/liter of water? [Re: tomcards]
#26618205 - 04/21/20 04:20 PM (3 years, 9 months ago) |
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Quote:
tomcards said: So that's a total of 40g/liter.
To clarify, the MEA that I'm using is the pre-mixed, all-in-one, just-add-water type.
Im pretty sure typical recipes call for 10g per 500ml. Thats 20 of MEA not including agar per liter. i am a little on the rich side of the unmodded recipe at 25.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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tomcards



Registered: 04/24/15
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Re: How much MEA/liter of water? [Re: Smartattack]
#26618286 - 04/21/20 05:05 PM (3 years, 9 months ago) |
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Here's the MEA, purchased from a reputable dealer:

Their description says, "Premixed dehydrated agar formula. 25 grams of media are recommended per 500 ml of distilled water."
That's 50 grams/liter, like I'm using, based upon Bod's tek.
It sounds like I'm using the right amount.
I'm so confused.
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poisoned
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Re: How much MEA/liter of water? [Re: tomcards]
#26618297 - 04/21/20 05:11 PM (3 years, 9 months ago) |
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Bod's tek calls for 20g/L of MEA
But you have a premixed formula, so there's also agar inside. I'm not sure what the mixture is, but 50g/l of that sounds within reasonable amounts.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,776
Loc: A thought
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Re: How much MEA/liter of water? [Re: poisoned]
#26618310 - 04/21/20 05:19 PM (3 years, 9 months ago) |
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Yes, if it includes agar powder then it all makes sense. In a nutshell, get out of here now and just follow the directions.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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tomcards



Registered: 04/24/15
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Loc: San Francisco
Last seen: 11 months, 6 days
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Re: How much MEA/liter of water? [Re: Smartattack]
#26618323 - 04/21/20 05:23 PM (3 years, 9 months ago) |
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Whew. Got it.
Thanks guys.
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shevanel
Gone til November


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Re: How much MEA/liter of water? [Re: tomcards]
#26618350 - 04/21/20 05:35 PM (3 years, 9 months ago) |
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I use roughly 20-25 mea for 500ml
I use same exact premix.
Edited by shevanel (04/21/20 05:37 PM)
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tomcards



Registered: 04/24/15
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Re: How much MEA/liter of water? [Re: shevanel] 1
#26618368 - 04/21/20 05:53 PM (3 years, 9 months ago) |
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Thanks for the reassurance Shevanel!
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