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OfflineMocha Magic Man
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Registered: 06/11/19
Posts: 4
Last seen: 2 years, 5 months
Hyphae interactions. cloning/isolation
    #26615641 - 04/20/20 03:06 PM (3 years, 9 months ago)

Greetings my esteemed fellow humans! Please forgive me if these questions have been asked/answered before. This is my first post and I am nervous that someone will point out some obvious post I have missed!! Anywho, I am currently trying to ensure potent, large yielding genetics (who isn't? :tongue:) by isolating some kickass genetics with agar. My main limiting factor here is petri dishes. I have made some reusable ghetto ones out of 1/2 pint jars (thank you PF tek) and have a sleeve of 20 sterile 100mm disposable plastic ones. So my main question is this: How can I best use my resources to find an optimal strain.. Which leads me to a few more questions. SO I have been doing my homework and I have not been able to figure out a few things. Essential I do not understand how Hyphae networks and different genetics integrate throughout a given substrate. So let's say I have a 5 qt jars of MS spawn mixed with coir and verm. Once the mycelium network has been established, to what degree do different parts have different genetics? I ask because I want to know the best way to take clones from a prolific flush and know which fruits will have the same genetics. And let's say I have a strain/variety that I want to keep WITHOUT isolating it further. How can I take an agar wedge (ultimately transfer to a master) without isolating? So I am just getting started with the whole agar thing and have lot's of ideas and questions and I am a bit overwhelmed with my options. Can anyone point me in the direction of where to start/what to focus on? I have 6 qt jars currently colonizing with PE and I was going to hopefully get a nice clone or few from those. Meanwhile I have a PE spore syringe I want to try and isolate from. To be more concise: Can anyone point me to a resource/tell me about Hyphae interactions in terms of trading genetics and fruiting? Which leads to: For getting a good clone with some magical genetics, is it a better idea to have multiple small minitubs or a couple medium sized or just one big monotub? I have 3 sizes available. I'll just leave it at that and see what comes of it! Thanks in advance!!! Oh yeah happy 4/20 :smile:
-MMM


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Offlinepoisoned
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I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: Hyphae interactions. cloning/isolation [Re: Mocha Magic Man]
    #26617028 - 04/21/20 08:01 AM (3 years, 9 months ago)

You'd get a lot more responses in Mushroom Cultivation. This isn't an advanced topic and most people don't even frequent here.

The best way to get some good cultures is to make some clones and test them out.


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How I do glass dishes


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OfflineJe77Ce11ar
I'm a teapot


Registered: 01/09/19
Posts: 244
Last seen: 4 months, 5 days
Re: Hyphae interactions. cloning/isolation [Re: poisoned]
    #26617688 - 04/21/20 12:38 PM (3 years, 9 months ago)

Like Poisoned said, this belongs in mush cult.

Apart from that i think youre putting the wagon before the horse here. Firstly idt itd be ideal to have a true isolate, youd want some genetic variation. Secondly, a new "strain" is created whenever two hyphae connect. In a mycelial network, genetic material and information is constantly being shared btwn and among cells. Every fruit body, even on a clone culture, is going to be genetically distinct. If you want potent mushrooms id suggest growing PE or one of its varieties or another species of psilocybe/panaeolus. Other than that, it takes time repeating the cycle of fruit, clone, print, fruit, clone, print to get a stable, good performing culture


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OfflineMocha Magic Man
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Registered: 06/11/19
Posts: 4
Last seen: 2 years, 5 months
Re: Hyphae interactions. cloning/isolation [Re: Je77Ce11ar]
    #26620641 - 04/22/20 05:26 PM (3 years, 9 months ago)

Thanks for your input! Every fruit body is genetically distinct?! I'll have to continue my research it seems! I know that information is shared between the mycelium network but of course it can't be ALL shared.. right? I mean among all the thousands of spores they can't all be compatible AND share info. Is a strain really just when hyphae connect? That is different from when they share nuclei and have two per cell? (Not sure how mating comes into play) I mean if they all shared info then they would have to be the type of mushrooms that don't have cell walls, no? Forgive my ignorance. I figured since it was about genetics and stuff that it belonged in the advanced but I shall definitely post onto the general forum!!


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OfflineJe77Ce11ar
I'm a teapot


Registered: 01/09/19
Posts: 244
Last seen: 4 months, 5 days
Re: Hyphae interactions. cloning/isolation [Re: Mocha Magic Man]
    #26623875 - 04/24/20 03:43 AM (3 years, 9 months ago)

Almost all mushrooms that we cultivate are basidiomycota, which have indistinguishable, compatible haploid cells in the form of mycelia (hyphae). Hyphae are the main mode of vegetative growth of a fungus and consist of several connected cells surrounded by a cell wall. Although connected, they are separated by pores called septa through which ribosomes, mitochondria, and nuclei can pass via the cytoskeleton.

These haploids grow and eventually connect via plasmogamy (the connection of cytoplasm of two parent cells). The connection of haploids is facilitated via clamp connections that help coordinate and re-establish pairs of compatible nuclei following synchronous mitosis. Reproduction occurs as nuclei are exchanged (karyogamy) and the resulting cell is now a dikaryon (read: new strain). These diploid cells usually grow and form basidiocarps (fruit bodies), and through meiosis (gamete production) produce spores.

The spores eventually find a suitable environment and start to germinate, producing tubular structures that turn into hyphae (monokaryons) and the cycle repeats. You are correct in the fact that these monokaryons need to be compatible with each other to connect. Depending on the species though (i'm not sure about Psil. Cubensis and not that good with genetics), these monokaryons can be compatible with up to 90% of other monokaryons.

Growing at home though, you probably shouldnt really be thinking this deep about mushroom reproduction, unless you're trying to start heavy lab work. I know Ryan Paul Gates of TerrestrialFungi works a lot with monokaryons in his work to get fruiting Cordyceps cultures.


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