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Offlinegism
ACIDBATH
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Registered: 08/20/19
Posts: 11
Last seen: 2 months, 15 days
agar issue
    #26616868 - 04/21/20 05:59 AM (3 years, 9 months ago)

ok i am having some agar issues guys, i’m seeing no growth and i don’t know why. i did some transfers from plates that were about a month old. all the plates i transferred from were clean plates. yeah a bit overgrown but i’ve taken transfers from plates that old before.. i’ve been doing agar about six months and i think i have a pretty good grasp on what i’m doing but now, i can’t get anything to grow on a plate. the only thing different is i’m using a different brand of malt powder. my recipe is 10g malt powder, 10g agar powder per 500ml. i even ran a batch of pda on 04-15 and those plates still aren’t showing growth either. i thought it might b the temp so i bought the temp up, no change.

help me guys i’m so frustrated i have a ton of plates sitting on my shelf and they’re not doing anything at all... someone mentioned senescence but i’m not sure if it would b that because i haven’t worked the hell out of these genetics. there’s a couple pin clones i took that did absolutely nothing either, a few tissue samples from another mush, all various different cubes in general and none are doing anything. the oldest ones are from 04-03 and they’re barely getting fuzzy..


also there’s no contam in the plates either that kinda strikes me
as odd as well.. i included some pics. i used mea and pda on several different batches. i took transfers from plates, i took tissue samples, i even took pins off some old plates as well and sadly still no luck






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OfflineFrankRhizo
Interdimensional Travel Agent
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Registered: 11/28/17
Posts: 295
Loc: Subterranean Flag
Last seen: 6 months, 22 days
Re: agar issue [Re: gism]
    #26616887 - 04/21/20 06:12 AM (3 years, 9 months ago)

Plates look good to me, I hope someone can weigh in, I feel for you man. Your transfer pieces look like they should take right off.


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OfflineClassicflh
Stranger

Registered: 03/02/19
Posts: 2
Last seen: 1 year, 17 days
Re: agar issue [Re: FrankRhizo]
    #26616909 - 04/21/20 06:32 AM (3 years, 9 months ago)

I had same problem with a batch of agar.  I reviewed several teks and figured out I was overcooking it - boiled too long.  I corrected that and my last batch is the best I've ever had.  Transferred to WBS and it ran very aggressively.  Good luck!


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