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OfflineSavoryGravy
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My bags stop growing
    #26615978 - 04/20/20 06:18 PM (3 years, 9 months ago)

I've done about 10 bags now. Not one has made it.

A couple were contaminated. Including one that didn't start growing bad stuff for two weeks, which I can't explain.

Most just stopped growing. The mycelium is still there, there's no contamination, it just stopped growing. I've tried different temperatures and different amounts of light.

My SWAG is not enough air exchange.I don't see how, but that's my best guess.

Please help.


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InvisibleWyoMX
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Registered: 07/06/15
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Re: My bags stop growing [Re: SavoryGravy]
    #26615988 - 04/20/20 06:22 PM (3 years, 9 months ago)

Are you making your bags or buying premade? Also how are you inoculating? Spore syringes or agar?


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Offlineshevanel
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Re: My bags stop growing [Re: WyoMX]
    #26616150 - 04/20/20 07:33 PM (3 years, 9 months ago)

Yes please explain the process, grain used and such.

Are you hanging the bags after sterilizing to help create a plenum of air above the grains? #1 reason a bag stalls is you have a vacuum #2 is too dry/wet #3 is bacteria.


Edited by shevanel (04/20/20 07:35 PM)


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Offlineahatchingegg
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Re: My bags stop growing [Re: shevanel]
    #26616247 - 04/20/20 08:14 PM (3 years, 9 months ago)

My bags stopped growing too. Turns out I was cutting off the air supply with the way I had them stored. Be sure that CO2 can escape the mycelium and get out of the filter. Once I did that, my colonization restarted on a couple of the bags.


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OfflineSavoryGravy
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Re: My bags stop growing [Re: ahatchingegg]
    #26617974 - 04/21/20 02:31 PM (3 years, 9 months ago)

(Thought I answered. Guess not.)

Thanks for the replies.

I'm making the bags with oats. Inoculating with agar.

I don't hang them but I've got a space in each one. They aren't stacked or anything, and the filter is exposed.

How can I make sure I get air exchange?


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Offlinepoisoned
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Re: My bags stop growing [Re: SavoryGravy]
    #26619394 - 04/22/20 06:31 AM (3 years, 9 months ago)

How do you inflate them when inoculating?


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How I do glass dishes


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OfflineSavoryGravy
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Re: My bags stop growing [Re: poisoned]
    #26621741 - 04/23/20 07:56 AM (3 years, 9 months ago)

Quote:

poisoned said:
How do you inflate them when inoculating?




Wut?

Take the bag out of the pressure cooker, put it in the SAB, take the filter out, drop the agar in, twist it up and tie it with wire. Put it on the shelf. Wonder what I'm doing wrong. Curse the mushroom gods.


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Offlinepoisoned
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Re: My bags stop growing [Re: SavoryGravy]
    #26621747 - 04/23/20 08:01 AM (3 years, 9 months ago)

Yeah, here's your issue. When you open them up to inoculate them with agar, they're receiving a huge amount of air. Remember, SAB is still a dirty place, it only relies on still air to prevent contaminants from getting anywhere. Once you open that bag up, you move a lot of air into it. If you want to inoculate your bags with agar, I'd recommend you to either preseal them or seal them immediately after you get them from the PC, hang them up so they get to inflate through the filter and only then inoculate them. Even then, opening bags without a flowhood will be very unreliable IMO.


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How I do glass dishes


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