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OfflineBobBee
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PE spore to agar very poor performance, why?
    #26615781 - 04/20/20 04:29 PM (3 years, 9 months ago)

I'm using this recipe and some strains do ok but PE specifically seems to be overrun with contams.

Does someone recommend a better recipe for PE, if it even matters?

Spore to Agar Recipe
½ pint mason jars
Foil and scale
¾ c water
3 g potato flakes
2 g agar agar
1 g corn syrup (~ 1 lg drop)
5 drops food color

Mix all but syrup. Heat to melt flakes. Add syrup. Do not boil. Pour in dishes. Place interior lid upside down, do not turn outer lid on too tight. Cover with foil. PC 15 psi 30 minutes. Leave in PC to completely cool at least 6 hours. Makes 6 dishes.


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InvisibleFooMan
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Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
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Re: PE spore to agar very poor performance, why? [Re: BobBee] * 1
    #26615900 - 04/20/20 05:34 PM (3 years, 9 months ago)

The strain and agar mix has nothing to do with contams. Your spore sample which I'm assuming is a syringe or swab is contaminated. Find a new source of spores or try to find some clean growth and transfer it until there are no contaminants.


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