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funchky
Determined
Registered: 04/19/20
Posts: 56
Last seen: 3 years, 4 months
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Graduating to Pans (on a budget)
#26615655 - 04/20/20 03:19 PM (3 years, 9 months ago) |
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Hello Shroomery. It's been a number of years and I couldn't remember my old handle, but I am back with a new username.
I have some basic experience with mushrooms (a few successful monotubs, and some isolation and cloning work with agar). Not an expert but I have an understanding of how to work sanitary/sterile.
I've read through quite a few long threads from the resident pan experts (thank you! Blue Helix, Azura, mary fairchild, JOC, just to name a few). I'm exploring a simple and cost effective tek that will not break the bank. More to come on that, but I'm starting to compile a spreadsheet with items and prices to budget the materials needed for each part of the process.
Considering the following workflows:
MS Syringe -> Agar -> Liquid Culture -> Sterilized Sub Bags -> Recovery -> Case / Fruiting Chamber (*Azura)
MS Syringe -> Grain Inoculation -> Spawn to Substrate -> Recovery -> Case / Fruiting Chamber
All new materials would be required so cutting out the agar and liquid culture work could save some time and money. I've knocked up grain with MS syringes in the past with a very high success rate, but i've never worked with pan spores.
For starters, Is it worth time and money to do the extra isolation steps for a pan grow or is it reasonable to start with MS inoculation to grains?
Edited by funchky (04/20/20 03:22 PM)
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jcm4620
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Registered: 05/26/19
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Re: Graduating to Pans (on a budget) [Re: funchky]
#26615893 - 04/20/20 05:30 PM (3 years, 9 months ago) |
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click my link it may help
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
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Loc: Equestria? Mordor? Wester...
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Re: Graduating to Pans (on a budget) [Re: jcm4620]
#26615938 - 04/20/20 05:58 PM (3 years, 9 months ago) |
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In sound mind, i could never recommend syringe to grain, but some people have success with it. Highly recommended not to skip agar. You could skip LC, but that's about it. The rest, JCM's tek or MacMerdin's PanCakes are about the least amount of work you can do.
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jcm4620
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Re: Graduating to Pans (on a budget) [Re: MLPismyOPSEC]
#26616056 - 04/20/20 06:48 PM (3 years, 9 months ago) |
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ya agar is a must with pans if you ask me. especially if running jams but they all can overlay if you dont have a good culture to start with. but the jams like to overlay somethin terrible. and even sometimes when you have a great looking plate that shit will still overlay on ya after casing. but there is at least a lesser chance of that by using agar compared to say knockin up grain jars with a syringe. which is risky in and of its self anyway as already mentioned
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funchky
Determined
Registered: 04/19/20
Posts: 56
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Re: Graduating to Pans (on a budget) [Re: jcm4620]
#26616139 - 04/20/20 07:24 PM (3 years, 9 months ago) |
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Quote:
jcm4620 said: click my link it may help
Thanks JCM... I really like your FC set-up. I estimated the parts total at around $215 ?? (similar parts)

I may try a hybrid between this and angam's FC using some cheaper parts. Do you think the tub would do better with only 1-2 bubble stones and a few small PC fans running on interval timers? This would mean just a single (smaller/cheaper) air pump in use.
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funchky
Determined
Registered: 04/19/20
Posts: 56
Last seen: 3 years, 4 months
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Re: Graduating to Pans (on a budget) [Re: jcm4620]
#26616157 - 04/20/20 07:37 PM (3 years, 9 months ago) |
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Quote:
MLPismyOPSEC said: In sound mind, i could never recommend syringe to grain, but some people have success with it. Highly recommended not to skip agar. You could skip LC, but that's about it. The rest, JCM's tek or MacMerdin's PanCakes are about the least amount of work you can do.
Quote:
jcm4620 said: ya agar is a must with pans if you ask me. especially if running jams but they all can overlay if you dont have a good culture to start with. but the jams like to overlay somethin terrible. and even sometimes when you have a great looking plate that shit will still overlay on ya after casing. but there is at least a lesser chance of that by using agar compared to say knockin up grain jars with a syringe. which is risky in and of its self anyway as already mentioned
Agreed. Agar is def more consistent/safe and fun to do. Would be starting from scratch so trying to keep cost down as much as possible.
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jcm4620
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Re: Graduating to Pans (on a budget) [Re: funchky]
#26616163 - 04/20/20 07:40 PM (3 years, 9 months ago) |
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well u shoulda joined a day sooner id have sent u a pan jam print to help get ya goin for free. pm me an address il put u on the next list
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jcm4620
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Registered: 05/26/19
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Re: Graduating to Pans (on a budget) [Re: funchky]
#26616184 - 04/20/20 07:49 PM (3 years, 9 months ago) |
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Quote:
funchky said:
Quote:
jcm4620 said: click my link it may help
Thanks JCM... I really like your FC set-up. I estimated the parts total at around $215 ?? (similar parts)

I may try a hybrid between this and angam's FC using some cheaper parts. Do you think the tub would do better with only 1-2 bubble stones and a few small PC fans running on interval timers? This would mean just a single (smaller/cheaper) air pump in use.
the more air stones the better fae is key. thats why i run 6 stones and 2 good pumps and this way gives me maximum fae while still keeping really high humidity. i have no cpu fans in my chambers. i use a ceiling fan in the room to keep air moving on 24/7 and i have a fan on a stand on 1 end that comes on for a few min every 3 hrs just for a evaporation boost and thats it. it actually is very low tech and low maint.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Graduating to Pans (on a budget) [Re: jcm4620] 1
#26616375 - 04/20/20 09:00 PM (3 years, 9 months ago) |
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may be cheaper to build a mini greenhouse. I got a roll of really thick crystal clear plastic for 5 bucks at Wally world. it was marked down. Got a coolmist reduced to 13 at the same place. Programmable digital timer was like 10 bucks at harbor freight but I already had a couple laying around. I'm going to either use the plastic shelves I already have or build a frame out of PVC then wrap in the plastic. I'll use either Velcro or strong magnets for the "door" then just slap in cool mist on the bottom shelf running 24/7. I may put a small fan on a timer and or the old warm mist(if I can find a use) on a timer. From there it will be all dialing in which I'm thinking will be the hard part. I've spent about $18 so far not including what I had laying around and I almost have enough if not already to set up the greenhouse. Just had to do a little searching and bargain shopping. The most expensive part will be either a mini greenhouse about 35 online or plastic shelves which I'm thinking are around $40 and go up from there. If you don't have a old humidifier laying around I always see a bunch of them at thrift stores. You can also pipe a humidifier into see totes or something too. Idk but where there is a will there is a way no matter what the budget.
-------------------- Trade List 🖕🖕🖕 6 hole Mini Monos
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jcm4620
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Re: Graduating to Pans (on a budget) [Re: gizmo1]
#26616655 - 04/21/20 12:28 AM (3 years, 9 months ago) |
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oh ya its way cheaper to build a gh. all the shit i have like i said in my write up was already layin in my garage from when i wasted so much $$ years ago when i started out. another plus about the gh is you have more room for more trays. if i had the room where im at id be using my martha set ups i already have. but my space is so limited here i can only fit the 2 fcs i was able to piece together out of what i already had layin around. i can only fit 4 monotubs or 2 of these and 2 monotubs in my tiny ass condo bedroom. but it works very well for me tho with the space i have. the only downside id say is the amount of trays i can fit. i can fit 2 lasagna size trays or 1 of my propagation tray in each fc. i think i still have enough shit in the garage to build 1 more but that would be pushing it. but yes this may be difficult on a tight budget. the only thing is tho that its very easy and easy to maintain.
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