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mushhead
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From agar to grain - My first agar log
#26604440 - 04/15/20 11:39 PM (3 years, 9 months ago) |
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So I recently started some agar plates with B+ Spore and they've been colonizing for about 5 days, I've had some good growth and decided to transfer some myc into a rye jar. I cleaned out my STB and got my jar, tools, and dish inside. I cut out a piece of myc (with some spores on it as well) and dropped it into the jar, I shook the chunk of agar into the side of the jar.
Now given everything works out well, given my cleanliness was enough, would this kind of inoculation succeed? I've only been working with agar for a few weeks now.
Furthermore if this does work, I'll keep this thread updated with pics, maybe I'll try a g2g again.
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Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
Edited by mushhead (04/18/20 07:30 PM)
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shevanel
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Re: From agar to grain? [Re: mushhead] 1
#26604463 - 04/15/20 11:50 PM (3 years, 9 months ago) |
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Most people would have made a xfer from the MS plate to a new agar plate[and again later]in order to clean up the growth.
What you did [can] work but you havnt minimized the risk of contams by having inoculate thats assured clean.
Edited by shevanel (04/15/20 11:52 PM)
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SFS96
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Re: From agar to grain? [Re: mushhead]
#26604467 - 04/15/20 11:52 PM (3 years, 9 months ago) |
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That’s how it’s done but you should really take multiple transfers to other plates before you go to grain. You might as well just put spores strait to a grain doing it the way you did. The point of agar is to transfer until your sure you have a clean culture. You don’t know if your culture is clean now because you used your germination plate to go to grain.
-------------------- How I make and preserve tea
Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood
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mushhead
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Re: From agar to grain? [Re: shevanel]
#26604469 - 04/15/20 11:55 PM (3 years, 9 months ago) |
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Quote:
shevanel said: Most people would have made a xfer from the MS plate to a new agar plate in order to clean up the growth.
Yeah I actually have other plates that I transferred to, but there was just a good amount left on the original plate so I thought why not? You haven't really answered my question, but one can assume the answer from your response that its a safe practice - tried and true? Perhaps, I have yet to experience it.
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shevanel
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Re: From agar to grain? [Re: mushhead]
#26604473 - 04/15/20 11:59 PM (3 years, 9 months ago) |
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Using the germination plate to inoculate grain isnt considered a safe practice by any stretch. Like the other person said. Its almost like using the spore syringe straight to grain. [Which can work] but youre using agar in your practice so thats what youre trying to avoid doing.
Edited by shevanel (04/16/20 12:01 AM)
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mushhead
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Re: From agar to grain? [Re: shevanel]
#26604482 - 04/16/20 12:06 AM (3 years, 9 months ago) |
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I agree with you, and I know the basic agar transfer process which I am following. I am not without an imagination so while doing transfers I decided to attempt to knock up a jar, did I do correctly? What I mean is when I have a few transfers and some beautiful plates is this the way you knock up your jars?
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SFS96
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Re: From agar to grain? [Re: mushhead]
#26604486 - 04/16/20 12:09 AM (3 years, 9 months ago) |
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Watch the last video here and you will know if u did it right.Easy af cult videos
-------------------- How I make and preserve tea
Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood
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shevanel
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Re: From agar to grain? [Re: mushhead]
#26604491 - 04/16/20 12:11 AM (3 years, 9 months ago) |
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That's one way. You got it.
I like to drop the wedges [1 plate per master for future G2G] right on top..no shake, no movement..when the myc recovers and later jumps onto the grain and gets a nice golf ball sized thats when i give it some help and shake it up.
Edited by shevanel (04/16/20 12:13 AM)
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mushhead
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Re: From agar to grain? [Re: SFS96]
#26604494 - 04/16/20 12:13 AM (3 years, 9 months ago) |
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Quote:
SFS96 said: Watch the last video here and you will know if u did it right.Easy af cult videos
I really appreciate it! I did it almost just as himself there so I'm sure i'll have some good results. I'll post em here why not?
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Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
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shevanel
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Re: From agar to grain? [Re: mushhead]
#26604495 - 04/16/20 12:13 AM (3 years, 9 months ago) |
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Please do. Everyone loves pics and talking about them.
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mushhead
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Re: From agar to grain? [Re: shevanel]
#26604496 - 04/16/20 12:15 AM (3 years, 9 months ago) |
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Quote:
shevanel said: That's one way. You got it.
I like to drop the wedges [1 plate per master for future G2G] right on top..no shake, no movement..when the myc recovers and later jumps onto the grain and gets a nice golf ball sized thats when i give it some help and shake it up.
Alright, hopefully my little shimmying it into the grain wasn't the best idea, but hey we learn.
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Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
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SFS96
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Re: From agar to grain? [Re: mushhead]
#26604505 - 04/16/20 12:20 AM (3 years, 9 months ago) |
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At least you got a little bit of practice maybe u will get some mushrooms too
-------------------- How I make and preserve tea
Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood
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shevanel
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Re: From agar to grain? [Re: mushhead]
#26604506 - 04/16/20 12:20 AM (3 years, 9 months ago) |
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Either way works. Its in there. I dont fuss with it because id rather not: A. Get the wedge stuck to the glass and. B. knock the myc off the wedge so that it recovers as a nice piece not a bunch of teeny tiny pieces if that makes sense.
Edited by shevanel (04/16/20 12:22 AM)
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poisoned
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Re: From agar to grain? [Re: shevanel]
#26604796 - 04/16/20 04:21 AM (3 years, 9 months ago) |
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Nothing wrong with dropping that plate to grain. Sure, it might contam, but you might also get shrooms quicker. And you get a practice run.
Do some risky attempts if you have a general plan on how to improve on those techniques.
Edited by poisoned (04/16/20 04:22 AM)
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mushhead
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Re: From agar to grain? [Re: poisoned]
#26605875 - 04/16/20 01:32 PM (3 years, 9 months ago) |
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Quote:
poisoned said: Nothing wrong with dropping that plate to grain. Sure, it might contam, but you might also get shrooms quicker. And you get a practice run.
Do some risky attempts if you have a general plan on how to improve on those techniques.
I appreciate the advice truly!

Some amazing growth here, this is the first plate I transferred to, the others are still recovering.
EDIT
 Only hours later. I also made up some more plates for the next transfers.
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Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
Edited by mushhead (04/16/20 05:45 PM)
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mushhead
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Re: From agar to grain? [Re: mushhead]
#26607835 - 04/17/20 10:42 AM (3 years, 9 months ago) |
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 Plate looks good, however I did spot some mold growing on the edges as you can tell. I was wondering if I should just grab another cutting after some myc growth or cut off the contams since they haven't sporulated yet.
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mushhead
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Re: From agar to grain? [Re: mushhead]
#26610425 - 04/18/20 12:27 PM (3 years, 9 months ago) |
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So I decided to just chop off the budding mold before it sporulated and had good results, no other croppings of the pesky hyphae have surfaced as of yet. My other transfers are doing quite well, notice the missing numbers of the ones that didn't quite do so well heh. I'm attempting further transfers today from other ms plates of B+. I'm having a lot of trouble procuring iso alcohol, I can't even get 70% anywhere let alone 91%, this has lead me to the only options available: Lysol, peroxide, hand sanitizer... anyone have any other suggestions that I can attempt to procure?
EDIT: Got my question answered elsewhere: Everclear 190 proof watered down....3 parts Everclear to 1 part water should get you around 70%. Also I ended up just buying 99.9% iso off ebay for an easier solution.
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Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
Edited by mushhead (04/18/20 07:31 PM)
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mushhead
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Re: From agar to grain? [Re: mushhead]
#26611891 - 04/19/20 01:33 AM (3 years, 9 months ago) |
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Got round two agar plates made up, decided to add a little color to these guys, green is better then basic MEA yellow >~>
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mushhead
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Re: From agar to grain? [Re: mushhead]
#26612916 - 04/19/20 12:27 PM (3 years, 9 months ago) |
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 This morning I took two transfers from this beauty and dropped them onto new agar - However I have some very promising growth on my first attempts!
    
Now at the start of this thread I asked about proper practice when transferring your agar into jars and I thought i'd check up on that to see how it was doing and I am tickled by the results.

No contamination in sight, wonderful looking myc growth; sure its a MS germination with no transfers at all, but I have high hopes for this jar.
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Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
Edited by mushhead (04/19/20 02:43 PM)
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D3_Myc
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Re: From agar to grain? [Re: mushhead]
#26615454 - 04/20/20 01:56 PM (3 years, 9 months ago) |
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Looking good! Off to a great start.
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