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Offlinemycothea
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Registered: 10/03/19
Posts: 17
Last seen: 3 years, 8 months
First Agar attempt in ketchup cups
    #26614922 - 04/20/20 10:30 AM (3 years, 9 months ago)

First try at pastywhite's agar tek scaled up 3x for a pour of 50 2.5oz condiment containers.

Gotta pour at around 140 else the lids will warp.

Cups were soak sterilized in alcohol is it needed? i don't know.

I didnt have any parafilm so I didn't use my dishes last night, but may and stack 3 high in ziplock bags.

Tossed agar in a flask and pc'd at 15psi for 35min.

Checked on them 6 hrs later, all setup nice, a couple had a drop of agar running up side and will be tossed.

Plan is to innoc by syringe through lid and place tape over injection hole.


Perfect? No.

Cheap? Yes very, 2.50usd for a 50 pack of cups.



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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: First Agar attempt in ketchup cups [Re: mycothea] * 2
    #26615047 - 04/20/20 11:20 AM (3 years, 9 months ago)

Iso doesn't sterilize. Unless the dishes came sterile and you poured in a sterile environment, they'll probably all show contams soon. I'd let them sit a week or so before committing spores to what is probably an exercise in futility.

While you wait, I'd suggest getting.g some glad mini rounds, Petri dishes, holy Grail dishes, or tiny Mason jars and following a proven Tek.


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OfflineFrankRhizo
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Registered: 11/28/17
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Re: First Agar attempt in ketchup cups [Re: verum subsequentis]
    #26615054 - 04/20/20 11:24 AM (3 years, 9 months ago)

:whathesaid:


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Offlinemycothea
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Registered: 10/03/19
Posts: 17
Last seen: 3 years, 8 months
Re: First Agar attempt in ketchup cups [Re: FrankRhizo]
    #26615812 - 04/20/20 04:51 PM (3 years, 9 months ago)

Sealed package, poured in a sab.

I have not found the glad minirounds anywhere


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OfflineFrankRhizo
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Re: First Agar attempt in ketchup cups [Re: mycothea]
    #26615832 - 04/20/20 05:03 PM (3 years, 9 months ago)

Jelly jars then.
Souffle cups aren't sterile are they?


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InvisibleSmartattack
C'mon man
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Registered: 12/21/18
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Re: First Agar attempt in ketchup cups [Re: FrankRhizo]
    #26615865 - 04/20/20 05:17 PM (3 years, 9 months ago)

"soak sterilize" may have done you in here. As suggested don't inoculate those for at least a week. If they are good after a week, you got a 75% chance they stay good IME.


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Offlineverum subsequentis
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Registered: 03/22/16
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Re: First Agar attempt in ketchup cups [Re: Smartattack]
    #26616556 - 04/20/20 10:57 PM (3 years, 9 months ago)

Yep.


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Offlinemr. whothehell
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Registered: 08/31/19
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Last seen: 11 months, 19 days
Re: First Agar attempt in ketchup cups [Re: verum subsequentis]
    #26616676 - 04/21/20 12:59 AM (3 years, 9 months ago)

I suggest you buy some PP5 container and sterilize them with your agar in the PC


Edited by mr. whothehell (04/21/20 12:59 AM)


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Offlineorange771
Registered: 01/26/11
Posts: 288
Last seen: 1 year, 7 months
Re: First Agar attempt in ketchup cups [Re: mr. whothehell]
    #26616706 - 04/21/20 01:58 AM (3 years, 9 months ago)

I got started with slime containers off amazon, they look like the ketchup containers but they are pp5 and can be PC’d. Once I got real Petri dishes with parafilm and media bottles I tossed those containers, sooooo much better.


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OfflineZionsMane
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Registered: 10/03/20
Posts: 6
Last seen: 2 years, 2 months
Re: First Agar attempt in ketchup cups [Re: mycothea] * 1
    #26975410 - 10/08/20 12:31 PM (3 years, 3 months ago)

Any update or check-in?

Curious how this turned out.


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