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Invisiblegenesis128
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MS Inoculation to Agar and DIRECT Transfer to Grain
    #26613380 - 04/19/20 04:46 PM (3 years, 9 months ago)

Can I get some of your guys experiences regarding this?

I want to inoculate an agar dish with a ms print. And without transferring to any more plates, I'd like to transfer that only plate to grains. Would the myc be too weak or would it be ok? Looking for a lot of experiences and advice.

...I would base my decision to transfer further based on all of your info.

Thank you


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Offlinemimir
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: genesis128]
    #26613415 - 04/19/20 05:05 PM (3 years, 9 months ago)

The whole point of going through agar is the ability of remove any contaminants and propagate clean mycelium indefinitely. Doing a single plate doesn't really accomplish any of the two, if that's how you plan to do it it's not worth the trouble.


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Invisiblegenesis128
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: mimir]
    #26613421 - 04/19/20 05:09 PM (3 years, 9 months ago)

I’m not worried about contaminants. If it contams then it contams. Hopefully it doesnt But the reason why im going the agar route is because i dont want to make syringes at the moment. For reasons...


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Offlinemimir
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: genesis128] * 1
    #26613424 - 04/19/20 05:12 PM (3 years, 9 months ago)

Well if a contaminant shows up on agar, are you still going to inoculate jars knowing you'll have to throw them out? If you have spore prints and can't make syringes for some reason I guess you don't have many alternatives, but you'll see contaminants way before the plate is fully colonized anyway


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InvisibleRoger Clemency
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: genesis128]
    #26613433 - 04/19/20 05:17 PM (3 years, 9 months ago)

It’s fine to do it that way, it’s just more risky than transferring a time or two to make sure your clean.


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Invisiblegenesis128
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: mimir]
    #26613435 - 04/19/20 05:18 PM (3 years, 9 months ago)

Naw i wouldnt use that plate if it contams for sure. Ew


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Invisiblegenesis128
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: Roger Clemency]
    #26613438 - 04/19/20 05:19 PM (3 years, 9 months ago)

Thanks for the feedback so far you guys. Please keep them coming


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OfflineA.k.aM
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: genesis128]
    #26613446 - 04/19/20 05:23 PM (3 years, 9 months ago)

Myc is myc. If it’s clean there’s no problem.

If you got a clean germ plate and used it that would be the same as using a spore syringe.


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Invisiblegenesis128
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: A.k.a]
    #26613466 - 04/19/20 05:33 PM (3 years, 9 months ago)

Yeah... i thought so. Just not sure why the first plate usually looks so tomentose and weird unless transferred to another plate...?


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OfflineA.k.aM
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: genesis128] * 1
    #26613468 - 04/19/20 05:34 PM (3 years, 9 months ago)

Cuz it’s tons of spores/genetics growing on top of each other. Once it gets thinned out over a couple transfers it looks more uniform.


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Invisiblegenesis128
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: A.k.a]
    #26613478 - 04/19/20 05:40 PM (3 years, 9 months ago)

Oooo nice


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Offlinehuangdeez
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: genesis128]
    #26613516 - 04/19/20 06:05 PM (3 years, 9 months ago)

I say just try it and post about what happens, cuz for newbies like me its good info.


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InvisibleHtaeh
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: huangdeez]
    #26613570 - 04/19/20 06:35 PM (3 years, 9 months ago)

I put a couple chunks out of a ms inoc'd plate which didn't appear to have any contams into two separate grain jars tonight (have other transfer plates growing healthily to fall back on). I wanted to see how it went also thus doing it. Not much to add but I spose I'll post back how it looks in a couple days or so haha.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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Invisiblegenesis128
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: Htaeh]
    #26613582 - 04/19/20 06:43 PM (3 years, 9 months ago)

Yes!!  :mushroom2:


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OfflineSockadin
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: genesis128]
    #26613620 - 04/19/20 07:01 PM (3 years, 9 months ago)

So you want fast mushrooms? MS to a LC and roll the dice. It sounds like that is what you are gunning for. LC seems quicker if you don't care about cleaning up the genetic variance and isolating away from contams.


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Invisiblealaskappalachian
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: Sockadin]
    #26613632 - 04/19/20 07:06 PM (3 years, 9 months ago)

Quote:

Sockadin said:
So you want fast mushrooms? MS to a LC and roll the dice. It sounds like that is what you are gunning for. LC seems quicker if you don't care about cleaning up the genetic variance and isolating away from contams.




Ain't it? :awesomenod: That's how I pumped out spawn for kiddie pools 20 years ago lol.  When it worked it was just glorious.  When it didn't there was no lemonade to be made...


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Offlinepoisoned
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: alaskappalachian] * 1
    #26614356 - 04/20/20 05:03 AM (3 years, 9 months ago)

I did that now when I was starting to grow again. Inoculated the plate with spores, did some transfers and threw that plate to grain.

I don't agree it's the same as going MS to grain. You get to see if there's any obvious contamination. Spores also germinate faster on agar. And if you introduced any contam spores when nnocing the jar, myc still has a headstart.


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InvisibleHtaeh
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: poisoned] * 1
    #26614376 - 04/20/20 05:17 AM (3 years, 9 months ago)

Exactly, you at least get the opportunity to observe the myc before hand. Yes there are a lot of genetics growing upon each other so its possible for contams to be buried. I had some plates which had no food colouring in and the agar went pretty clear, when I put a light beneath them I could see on two of the ms plates an area of a contam, as an attempt to garner more experience I transferred from those two plates onto others and they are now looking healthy; I had two other ms plates which appeared to have no contams, the plates were deliberately made very thin and as they appeared clean I decided to practice grain prep and A2G transfer from one and the other one as a transfer to new food coloured plates which are looking brilliant, my best plates to date. So should my A2G transfer succumb to contamination I'll have first hand experience to avoid doing it in the future from a ms plate. And as stated have plenty of healthy plates to fall back on.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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Invisiblegenesis128
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: Htaeh]
    #26625274 - 04/24/20 05:27 PM (3 years, 9 months ago)

Bump... hteah... how are those ms jar coming along??


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Invisiblemushhead
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Re: MS Inoculation to Agar and DIRECT Transfer to Grain [Re: genesis128]
    #26625351 - 04/24/20 06:11 PM (3 years, 9 months ago)

Hey genesis128 I've done a few things with MS directly to grain. Yes it does work and you can check out my thread about it to see, however I strongly suggest you make a few transfers to build strong myc.

https://www.shroomery.org/forums/showflat.php/Number/26604440


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Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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