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Offlineaspiringmyconoob
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Registered: 04/18/20
Posts: 40
Last seen: 3 years, 8 months
Re: are my grain bags contaminated? please help [Re: Mellowbie] * 1
    #26612793 - 04/19/20 11:41 AM (3 years, 9 months ago)

Hey mellowbie,

I wish I could give you sound advice, but I'm a beginner myself. It sounds like you've had some experienced people give you your answer though. I hope you can eventually get everything to work out. Also, this site is somewhat difficult to navigate, but hey, you at least have nice full size pics unlike my small lower quality ones. It took me forever to change the file extension and reduce the file size small enough to even get the pics on here lol. Best of luck to you.


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InvisibleHtaeh
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Registered: 10/14/18
Posts: 320
Re: are my grain bags contaminated? please help [Re: aspiringmyconoob]
    #26612796 - 04/19/20 11:43 AM (3 years, 9 months ago)

No problem at all glad to see you here and happy I could help.

As anyone here can tell you; you learn more from mistakes than success. Thoroughly reading an aspect of a grow can help prepare you and reduce the likelihood of said disappointing mistakes. Ultimately, embrace your short comings, I can tell you that sub preparation in my opinion is one of the easier sides to this but everyone is different, don't be afraid to try your hand at sub preparation because at the end of the day the quicker you get it under your belt the better it will be for you and your mushies (and their quantity :wink:). I tried my hand today at oat preparation for the first time to take agar to bulk, I'm waiting for the PC to cool down and I could have well fucked it but ah well I'll learn what to do different next time :smile:.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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Offlineaspiringmyconoob
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Registered: 04/18/20
Posts: 40
Last seen: 3 years, 8 months
Re: are my grain bags contaminated? please help [Re: Htaeh] * 1
    #26612828 - 04/19/20 11:55 AM (3 years, 9 months ago)

I definitely believe that you're probably right that you learn more from your mistakes. You mess up once and you won't do it again, usually haha. I will start researching preparing my own grain and substrate then. I know that one day I will need to make agar instead of spore syringes, and I will definitely need to be making my own grain then so I can inoculate the grain with the agar. I'm definitely getting ahead of myself there, but then again, I am an aspiring myco noob lol.

You've definitely inspired me to expand my knowledge and to take some risks by trying new skills. I really hope your oat preparation goes as planned. My skills and knowledge seem elementary to your seemingly doctorate level lol. Thanks again for everything


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InvisibleHtaeh
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Registered: 10/14/18
Posts: 320
Re: are my grain bags contaminated? please help [Re: aspiringmyconoob] * 1
    #26612862 - 04/19/20 12:09 PM (3 years, 9 months ago)

Quote:

aspiringmyconoob said:
I definitely believe that you're probably right that you learn more from your mistakes. You mess up once and you won't do it again, usually haha. I will start researching preparing my own grain and substrate then. I know that one day I will need to make agar instead of spore syringes, and I will definitely need to be making my own grain then so I can inoculate the grain with the agar. I'm definitely getting ahead of myself there, but then again, I am an aspiring myco noob lol.

You've definitely inspired me to expand my knowledge and to take some risks by trying new skills. I really hope your oat preparation goes as planned. My skills and knowledge seem elementary to your seemingly doctorate level lol. Thanks again for everything





Lol you may make the same mistake once or twice but as long as you know where you go wrong you have an opportunity to progress. In this instance I'd say purchasing the premade sub is where you went wrong :wink: Truthfully, even beginning with PF tek is brilliant for learning the ropes of concepts, do's and don'ts, I personally thoroughly enjoyed my PF tek experience and had huge success (still many lessons learnt).

PF tek might not be what you want to do which is up to you but I believe its more reliable rather than going from an ms syringe straight to sub. Agar is what I've began delving into the passed couple of weeks, I can't stress enough how great it is, if this is hobby is something you're going to pursue my recommendation would be get some pf cakes on the go (to get your hands on some mushies :wink:) and whilst that's ongoing get learning agar quick quick for your bulk grows. I'm by no means an expert at all, I to am just getting my foot in the door with a lot to learn and re-learn hahah, its a journey my friend enjoy it! Feel free to drop me a message should you ever need help answering something, I'll always do my best to help.


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Offlineaspiringmyconoob
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Registered: 04/18/20
Posts: 40
Last seen: 3 years, 8 months
Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26612874 - 04/19/20 12:13 PM (3 years, 9 months ago)

Hey, thank you very much for coming in and helping out by answering my question and helping another beginner. Since you stated that the grain looks dry, do you think I should soak it before I add it to my monotub? I've seen where people will cut the top of the grain bag off and fill it with water and let it soak before they add it into their substrate. Should I mist my substrate when I first mix the grain into it and then leave it alone, or only add moisture and humidity during fruiting?

Also, I will be keeping my monotub in the laundry room, the same place the grain bags currently are. Do you see any potential harm by me doing laundry in the same room where the monotub is colonizing? I'm just worried about the chemical smell from the detergent, bleach, and ammonia and with whatever is floating around while the laundry is going. Could the tub become contaminated just by enough gas and air coming in through the lid? The lid has no seal and fits imperfect so there will be just enough of a gap for gas exchange. The holes will be covered with packing tape while it's colonizing.

Any and all help is greatly appreciated, thank you very much.


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Offlineaspiringmyconoob
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Registered: 04/18/20
Posts: 40
Last seen: 3 years, 8 months
Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26612920 - 04/19/20 12:27 PM (3 years, 9 months ago)

Sorry, I think I may have somehow sent a reply to you that was meant to go to someone else.

I originally looked into the pf tek, but I kind of made an ambitious risk of going with the grain bags and premade substrate. My reasoning, as a rookie, was if I'm going to do this I might as well go with the method that could potentially create a much larger harvest. Maybe I should get some small pf tek projects going as well.

Are spore syringes from an established vendor really that risky? I have like a 1/4 of one left and another unused one. I was going to use the unused one to inoculate more grain, but since it's sat for a month should I just get another one? And is premade substrate really that risky too? Maybe I should fast track a course to substrate production. I'm going to take the used syringe and inject a few areas in the ground outside somewhere where mushrooms grow a lot. I'm going to take a bag of substrate I bought like an idiot when I first got the syringe and spread the substrate over the area where I put the spores. I'll attempt it once the weather is warmer. I know there is a very small chance of growth and the spores probably need more than the dirt and substrate, but mushrooms grow their already and I'm not going to use them another way so hey, I might as well try it lol.


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InvisibleHtaeh
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Registered: 10/14/18
Posts: 320
Re: are my grain bags contaminated? please help [Re: aspiringmyconoob]
    #26612949 - 04/19/20 12:42 PM (3 years, 9 months ago)

Quote:

aspiringmyconoob said:
Hey, thank you very much for coming in and helping out by answering my question and helping another beginner. Since you stated that the grain looks dry, do you think I should soak it before I add it to my monotub? I've seen where people will cut the top of the grain bag off and fill it with water and let it soak before they add it into their substrate. Should I mist my substrate when I first mix the grain into it and then leave it alone, or only add moisture and humidity during fruiting?

Also, I will be keeping my monotub in the laundry room, the same place the grain bags currently are. Do you see any potential harm by me doing laundry in the same room where the monotub is colonizing? I'm just worried about the chemical smell from the detergent, bleach, and ammonia and with whatever is floating around while the laundry is going. Could the tub become contaminated just by enough gas and air coming in through the lid? The lid has no seal and fits imperfect so there will be just enough of a gap for gas exchange. The holes will be covered with packing tape while it's colonizing.

Any and all help is greatly appreciated, thank you very much.





Let me first start by saying this will need someone much more experienced answering because, firstly, I have no experience with monotubs and secondly, looking to get some shoebox grows under my belt before considering going for a mono. However, I'll still answer as best I can but im sure someone else will pitch in with a better answer.

What you describe about the cutting of the bag and filling with water, sounds to me like a 'dunk' a full hydration of the myc. I don't use bags and have only done dunks upon PF cakes before fruiting, so I cant answer that with any certainty, also in my mind dunking with uncolonized grain could be a vector for contam and in PF tek its often a last resort to try colonise exposed sub before fruiting. 

When you mix the spawn into the sub I don't believe its recommended to mist, this again is a vector for contam to uncolonized sub. I'm not entirely sure how the mono process goes but with shoeboxes you leave the tub closed off from FAE (or any considerable FAE) to colonise for ten to fifth teen days. The water from within the sub will feed the mycs efforts to colonize the bulk sub. Then put it in fruiting conditions. So moisture and FAE in fruiting.

While I cant imagine the laundry room will cause any adverse affects on the colonisation or fruiting of your tub im sure they'd prefer it without, but you can only do what you can do. Maybe just be mindful when doing the laundry and avoid throwing handfuls of washing powder around the room :wink:.

Also I've read people put colonizing tubs inside bin bags, which then the laundry rooms smell wouldn't matter so much.

Sorry I couldn't give a more certain response to your questions but im sure someone else can!

____________________________________________________________________________________________________


After responding to your message I have since seen it may not have been mine to answer :laugh2: but nevertheless it is there.

In response to your new comment; When I first began, I too wanted bulk right away but I did read a lot and became confident that even with PF I'd have a large amount of mush to enjoy so with the hopes of being thorough in learning I jumped in. You can make as many cakes as you like, I never kept score of exactly how much I grew in total over many cakes but it was plenty enough to keep me going for a year, different cakes birthed at different times. Got into a cycle of one cake finishing fruiting the next was ready to harvest, and the next ready to birth and take the old ones place. But there is nothing wrong with going straight to bulk but the general recommendation here would be to do it with agar, that's not to say you cant try with an ms syringe because it is doable but as some say here its a shot in the dark.

Those spore syringes keep for longer than you'd think, and they last much much longer with agar work. As an example I've bought three in total since starting this hobby, the golden teacher and B+ went all on PF jars (20ml syringes) I recently started agar as I said and bought an A+ syringe to inoc my plates with, as of today I have 13 healthy plates of A+ and ive used less than 3cc of the syringe, it lives in the fridge.


In regards to dumping off some spores in your local area, why not, you may one day in the future stumble upon them ready to pick :wink:


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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OfflineScared newbie
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Registered: 04/17/20
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Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26613322 - 04/19/20 04:23 PM (3 years, 9 months ago)

Is it okay 2 mix up spawn bag a second time???


--------------------
Scared Newbie, just trying to soak up all the skills I can  while learning the trade... Sorry in advance for all my questions! I'm sure plenty are to follow...


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OfflineScared newbie
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Registered: 04/17/20
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Loc: Michigan Flag
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Re: are my grain bags contaminated? please help [Re: Scared newbie]
    #26613344 - 04/19/20 04:32 PM (3 years, 9 months ago)

Spawn bags are on day number 12. This morning I noticed tiny bluish-green Spec's in a couple places. I mixed the bags up yesterday. Is it too late to start growing contamination or Never Too Late? Hopefully it's just bruising! But weird how it's only in small places facts are definitely too small for my camera here's a picture of my bag


--------------------
Scared Newbie, just trying to soak up all the skills I can  while learning the trade... Sorry in advance for all my questions! I'm sure plenty are to follow...


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Offlinehuangdeez
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Re: are my grain bags contaminated? please help [Re: Mellowbie]
    #26613576 - 04/19/20 06:37 PM (3 years, 9 months ago)

yo that second photo had some pink that looked hella funky...


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Offlinerumfor69
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Registered: 08/05/11
Posts: 6,842
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Re: are my grain bags contaminated? please help [Re: huangdeez]
    #26613869 - 04/19/20 08:54 PM (3 years, 9 months ago)

You didn't need to shake it up a second time it's only going to make it
take longer. Contamination can grow at any stage and any time


--------------------

Links For Beginners And Beyond:mspoil:


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OfflineMellowbie
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Re: are my grain bags contaminated? please help [Re: aspiringmyconoob]
    #26614228 - 04/20/20 01:53 AM (3 years, 9 months ago)

Thanks! It is definitely tricky, for sure... Best of luck 😊


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Offlineaspiringmyconoob
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Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26622410 - 04/23/20 01:44 PM (3 years, 9 months ago)

Sorry I took forever to respond, I've been pretty busy the past few days. I appreciate the information, I definitely will not be dunking or misting before the substrate is fully colonized and I might use a bin bag just to be safe.

I probably should have started off smaller, but it's definitely too late now. I think I'll try out a few jars too, especially since you've had a lot of luck.

My syringes have been in a cabinet for the past 5 weeks so I'll put them in the fridge then, hopefully it's not too late to store them in the fridge.

Yeah, it would be awesome to have some grow outside without me doing anything but the initial inoculation, it would be like Christmas lol.


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Offlineaspiringmyconoob
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Re: are my grain bags contaminated? please help [Re: Scared newbie]
    #26622423 - 04/23/20 01:48 PM (3 years, 9 months ago)

The little blue specs could be bruising of the mycelium. After I shook my bags, I was picking the bags up a couple times a day some days inspecting them and looking for progress and contamination. I noticed little blue spots about the size of the tip of a ballpoint pen in random areas in my bags. I initially thought it was mold or something. I researched it and am pretty sure it was bruising from the shake and picking the bags up too often. Since I've left them alone, the spots have almost completely gone away. Hope I am right and hope yours is the same problem. Best of luck to you


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InvisibleHtaeh
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Registered: 10/14/18
Posts: 320
Re: are my grain bags contaminated? please help [Re: aspiringmyconoob]
    #26622646 - 04/23/20 03:17 PM (3 years, 9 months ago)

It's no problem at all. Go big or go home lol but just always learn as you go and things will just get easier and much more rewarding. I'd say the syringes are probably fine, my fridge is set to 3 degrees Celsius, I think it needs to be between 2-8.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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Offlineaspiringmyconoob
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Registered: 04/18/20
Posts: 40
Last seen: 3 years, 8 months
Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26622763 - 04/23/20 04:07 PM (3 years, 9 months ago)

Ok very good, I appreciate it. I'm not sure if you read the above post, but I was wondering if you've have any information or could clarify this. After I mixed the bags, I was handing the bags like twice a day looking for growth and contamination. I noticed a few days ago that I had little circular blue spots about the size of a ballpoint pin in different areas throughout the grain bags. I thought it was mold or something, but upon further research, I think the spots are bruising from the mixing and from handling the bags too often. I also read that the bruising could be caused by condensation dripping and collecting and or from dry substrate from evaporation, so they kind of go hand in hand. I've left the bags alone and decreased the room temperature one degree which has reduced the swing in  temps and condensation has improved, so I believe the moisture has been returned back to the grains/mycelium instead of collecting on the walls of the bag. Since leaving the bags alone, the mycelium has continued to grow and the small blue spots are disappearing. I really hope it isn't mold.


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InvisibleHtaeh
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Registered: 10/14/18
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Re: are my grain bags contaminated? please help [Re: aspiringmyconoob]
    #26622896 - 04/23/20 05:01 PM (3 years, 9 months ago)

I believe if it was mold you'd likely notice a stall in growth, you'd certainly notice the mold grow. So if it has retreated so to speak then it was probably some myc bruising but that being said I don't know exactly if your grain myc can bruise, like I'm unsure if agar myc can bruise, but I don't see why not. I wouldn't be all that worried if I were you :smile:.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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Offlineaspiringmyconoob
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Registered: 04/18/20
Posts: 40
Last seen: 3 years, 8 months
Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26623047 - 04/23/20 05:57 PM (3 years, 9 months ago)

I did notice a stall in growth, but I think it was from the shake, moving the grain too much to check for contamination and growth, and from too big of temperature changes resulting in increased and decreased condensation throughout the day, at least I hope lol. I think I'll probably spawn it to my monotub in the next few days. I definitely did not see the spots get any bigger, they have just slowly gone away.


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InvisibleHtaeh
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Re: are my grain bags contaminated? please help [Re: aspiringmyconoob]
    #26623917 - 04/24/20 04:28 AM (3 years, 9 months ago)

Yeah after a shake the myc needs some time to recover but if they go back to normal growing speed a little while after the shake then you're all good.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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Offlineaspiringmyconoob
Stranger
Registered: 04/18/20
Posts: 40
Last seen: 3 years, 8 months
Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26624295 - 04/24/20 09:49 AM (3 years, 9 months ago)

Ok so I'm kind of freaking out. I was checking my bags this morning and noticed little white spots in one of my bags. I'm not sure if its the mycelium forming pins and primordia or if its trichoderma or something else. I know if bags have been colonized for too long they can begin to show pins. I really hope to God that it's pins and not  something else. If anyone could help me out I would greatly appreciate it. 





If these are pins, is it ok to spawn it to a monotub or will the pins decay and cause problems?


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