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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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Offlinepoisoned
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Registered: 04/17/13
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G. lucidum rescue project
    #26612784 - 04/19/20 11:39 AM (3 years, 9 months ago)

I had a very contaminated slant of G. lucidum. Took some transfers, including wood, trying to cover with another piece of agar, to see if I could get any culture out of it. That's what the best plate looked like:


I didn't manage to seal the top layer of agar, but it still seemed to work. As soon as I saw some growth on top of the agar slice, I did another transfer to 3 plates. 2 were very bacterial, but one showed growth:


T2 looks like this (that's not a satellite, it's on the lid):


Anyways, I thought that culture was unsalvagable, but it worked.


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OfflineForresterM
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Re: G. lucidum rescue project [Re: poisoned]
    #26612970 - 04/19/20 12:51 PM (3 years, 9 months ago)

That's awesome.  When it's just bacteria, you can usually just let reishi overrun it and it will eat it, no real hurry to transfer there.  Not so good against molds though...


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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinepoisoned
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Posts: 1,738
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Re: G. lucidum rescue project [Re: Forrester]
    #26612995 - 04/19/20 01:01 PM (3 years, 9 months ago)

There was so much bacteria and yeast that it was all surrounded with it when I was doing my previous attempts without covering it over with another slice of agar. Never got any real growth from it.


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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder


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