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metaphoric
Mr. Bater



Registered: 05/18/15
Posts: 730
Last seen: 2 years, 2 months
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Questions about this 100cc syringe
#26612603 - 04/19/20 10:14 AM (3 years, 9 months ago) |
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Here's the syringe I'd like to buy:
3 Pack 100ml Syringes luer Lock, Large Plastic Syringe for Scientific Labs and Liquid Dispensing Metric Multiple Uses https://www.amazon.com/dp/B07JL1Q4YD/ref=cm_sw_r_cp_apa_i_JAhNEbZPDEQPS
1st question: would you recommend this? I'm primarily looking to make GLCs from agar inoculated grain jars.
2nd question: do you think it comes with needles? It doesn't say anywhere so I would think it does.
3rd question: would the needles from my luer lock vendor spore syringes work?
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Questions about this 100cc syringe [Re: metaphoric]
#26612612 - 04/19/20 10:19 AM (3 years, 9 months ago) |
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1 Yes they're great for large amounts of LC
2 No they don't
3 Yes. 14-16g needles are better for thicker LCs
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Quick WBS Prep
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The Blind Ass
Bodhi



Registered: 08/16/16
Posts: 26,657
Loc: The Primordial Mind
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Re: Questions about this 100cc syringe [Re: metaphoric]
#26612613 - 04/19/20 10:20 AM (3 years, 9 months ago) |
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For needles/syringes go to your local grocery store pharmacist and ask for any they are about to throw out or don’t use don’t want.
I’ve been given many over the years for free, or like 20-50-100 for a dollar or so . I generally go to Foodlion to do this.
Worth a shot before buying. That’s my recommendation.
Edited by The Blind Ass (04/19/20 10:21 AM)
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metaphoric
Mr. Bater



Registered: 05/18/15
Posts: 730
Last seen: 2 years, 2 months
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Re: Questions about this 100cc syringe [Re: The Blind Ass]
#26612619 - 04/19/20 10:25 AM (3 years, 9 months ago) |
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FooMan 1) how long would you recommend I PC that syringe when it's full of water?
2) Do you recommend a whatman-type filter, poly filter, or no filter on your LC jars?
And thanks for the tip Blind 👍
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Questions about this 100cc syringe [Re: metaphoric]
#26612628 - 04/19/20 10:31 AM (3 years, 9 months ago) |
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20 minutes is plenty. You don't need a filter but I prefer Whatmanns for LCs
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Quick WBS Prep
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metaphoric
Mr. Bater



Registered: 05/18/15
Posts: 730
Last seen: 2 years, 2 months
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Re: Questions about this 100cc syringe [Re: FooMan]
#26612823 - 04/19/20 11:54 AM (3 years, 9 months ago) |
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Foo one more question for you. I already started a bunch of LCs just for shits n' giggles because I had leftover MS liquid and jars after starting my agar.
They are quart jars with SHIPs and no filter, filled with 600ml of broth. My question is will I run into difficulty extracting 100ml? Am I going to need to inject 100ml of sterile water first so I don't create a vacuum?
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Questions about this 100cc syringe [Re: metaphoric]
#26613082 - 04/19/20 02:08 PM (3 years, 9 months ago) |
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If your lid is airtight it'll definitely form a vacuum. I would suck air into the syringe while flaming the tip or PC the syringe empty with the plunger pulled back to push clean air into it rather than water.
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Quick WBS Prep
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metaphoric
Mr. Bater



Registered: 05/18/15
Posts: 730
Last seen: 2 years, 2 months
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Re: Questions about this 100cc syringe [Re: FooMan]
#26613111 - 04/19/20 02:29 PM (3 years, 9 months ago) |
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Oh really? Okay interesting you hear you say that, I'll do that.
What about sterilizing broth in my syringe so I can pull a 100CCs once every week without ever running out?
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