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OfflineFungiFart
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Mislabeled Spores? What is this? * 1
    #26612496 - 04/19/20 09:10 AM (3 years, 9 months ago)

Since I'm new to mycology, I'm trying all sorts of experiments. Recently I tried growing blue oysters using PF Tek. However, large white blobs are growing on the cakes.  Large yellow water droplets are also forming on the growth. It smells like urine, which is kindof funny to me.  I've read that shiitake can smell that way due to sulfur. 

Either my spore vendor sent me the wrong spores, or I messed this up.  Can anyone identify this fungus?



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OfflineLogicaL ChaosM
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Re: Mislabeled Spores? What is this? [Re: FungiFart]
    #26612502 - 04/19/20 09:13 AM (3 years, 9 months ago)

:mindblown:

:whattefuck2:

:incredible:

Alien Eyes. They can see now! :greyalien:


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OfflineSockadin
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Re: Mislabeled Spores? What is this? [Re: LogicaL Chaos] * 1
    #26612507 - 04/19/20 09:15 AM (3 years, 9 months ago)

They are bacterial and this is the Mycelium fighting off the infection.


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OfflineFungiFart
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Re: Mislabeled Spores? What is this? [Re: Sockadin]
    #26612529 - 04/19/20 09:25 AM (3 years, 9 months ago)

Interesting thanks. Is there anything I could do to help the mycelium fight the bacteria or is it a lost cause?


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InvisiblebodhisattaMDiscordReddit
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Re: Mislabeled Spores? What is this? [Re: FungiFart] * 2
    #26612549 - 04/19/20 09:34 AM (3 years, 9 months ago)

Start over


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OfflineFungiFart
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Re: Mislabeled Spores? What is this? [Re: bodhisatta] * 1
    #26612865 - 04/19/20 12:10 PM (3 years, 9 months ago)

Thanks,  I figured that would be the case.

To learn from my mistake, where do you think the bacteria came from?

  • For the PF Tek, I used an 8oz jar and there were zero signs of contamination before birthing.
  • When birthing the cake, my work area was sterilized and I used gloves.
  • I dunked the cake invitroly using distilled water.
  • After a 24-hour dunk, I rolled the cake in vermiculite
  • The tote I placed it in has perlite (lightly mixed with distilled water) and a layer of foil.
  • I would spray the walls of the tote and fan it when needed.
  • The only discoloration is from the water droplets.


Do you see any mistakes in my process?

Here is a recent photo.  I started to think they accidentally gave me lion's mane because of the way it was growing.


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OfflineLogicaL ChaosM
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Re: Mislabeled Spores? What is this? [Re: FungiFart] * 1
    #26612867 - 04/19/20 12:11 PM (3 years, 9 months ago)

Some serious alien infections going on there :eek:

Never seen anything like it in my life....

Are infections becoming more psychedelic over time? :mindblown:


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OfflineSockadin
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Re: Mislabeled Spores? What is this? [Re: FungiFart] * 2
    #26612872 - 04/19/20 12:12 PM (3 years, 9 months ago)

inoculated cakes via MS syringe. It was in step 1.


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OfflineFungiFart
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Re: Mislabeled Spores? What is this? [Re: Sockadin] * 1
    #26612914 - 04/19/20 12:26 PM (3 years, 9 months ago)

Logical Chaos:  haha yeah, it was pretty trippy looking.  I'm glad this was isolated from my other projects.  If you look closely at some of the photos you can see the hyphae cupping the droplets.


Sockadin:  Thanks. I had another project ruined by the inoculation process. Seems like everything that came from the one vendor was bunk. Since switching to someone new, I've had much more success.


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OfflineSockadin
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Re: Mislabeled Spores? What is this? [Re: FungiFart] * 2
    #26613000 - 04/19/20 01:03 PM (3 years, 9 months ago)

Yeah it can happen. Can't blame the vendors though, they only sell spores for microscopy and not Cultivation so bactiera is part of MS. Just keep swimming.


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OfflineFungiFart
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Re: Mislabeled Spores? What is this? [Re: Sockadin]
    #26613058 - 04/19/20 01:48 PM (3 years, 9 months ago)

Very true, I didn't look at that way.  However, I wouldn't mention any names as it very well could be my lack of knowledge that caused the contams. Thanks again for taking the time to give advice. On to the next project.


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