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Offlineaspiringmyconoob
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Registered: 04/18/20
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are my grain bags contaminated? please help * 1
    #26610993 - 04/18/20 04:38 PM (3 years, 9 months ago)

Hi everyone,

I am new to this website and to mycology. I am still discovering how to navigate the website and forums so I apologize if something similar has been asked and answered previously, I promise I will improve and become more knowledgeable with all of your great help. I have already learned so much from you guys.

I inoculated 5 one pound rye berry grain spawn bags a month ago yesterday with a spore syringe from a respected vendor. A week ago yesterday, so after 3 weeks of mycelial growth, I mixed the spawn bags up. The recovery has seemed to be somewhat slow and maybe even slightly stalled. The mycelium has continued to colonize the berries, but it just seems slow. I have the bags in the dark in a small laundry room where the window is covered, and I'm using a space heater for heat. The room has been around 74-76 degrees, but there has been a few times when the heater has shut off and then a couple hours or so go by before it gets turned back on. Yesterday I noticed some condensation in the bags. We recently had a cold spell come through and I did not realize that the temperature in the room dropped to 70, so I had to adjust the space heater to get the heat back up. The condensation has greatly improved, but there is still some condensation in the bags.

I'm afraid that the seemingly stalled growth and the condensation may be a sign of contamination. I will show pictures right before I mixed the bags and then pictures I took today, 4 weeks and one day post inoculation and one week since mixing the bags. The mycelium looked very healthy and strong before mixing. Oh, I also forgot to mention that when I do laundry, I have been removing the bags from the laundry room and place them in a cabinet in the kitchen where it is unfortunately cooler while I do laundry because I was afraid that the chemical small and whatever floating in the air during laundry might enter the bags through the filter and cause contamination or the chemical fumes from the laundry might interfere with the colonization. The laundry room is unfortunately the only room I have to use. Is it ok to leave the bags in the laundry room while I do laundry?

Any and all help and tips and tricks would greatly be appreciated. I would be so disappointed and devastated if I had to start over, but if I need to then I'll do what I need to do. Also the quality of these images has been drastically decreased to be able to upload them, but if anyone needs the original high quality images then let me know and I can give them to you. I posted this initially in the "just beginning" forum, but then I realized I probably should have posted it in the "mushroom cultivation" forum. I apologize for any confusing or if I'm somehow not following rules or need to do something different. I'm a definitely a newbie.

























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Offlinerumfor69
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Re: are my grain bags contaminated? please help [Re: aspiringmyconoob] * 2
    #26611043 - 04/18/20 05:05 PM (3 years, 9 months ago)

Indirect light is good at all stages of growth. There's never a need for darkness.
Room temp lower seventies Β°F is good don't go hotter.

Condensation is normal and is fine.
Bouncing back slow isn't necessarily a bad thing. Most contams grow fast.
The laundry room fumes from detergent wont hurt them but again a nice
stable place with indirect light and no perfume fumes would be better.

All you can do is wait to see if they colonize 100%. Working from spores
is always a dice roll on contamination anyhow and overall I think it's ok
so far.


--------------------

Links For Beginners And Beyond:mspoil:


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InvisibleHtaeh
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Registered: 10/14/18
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Re: are my grain bags contaminated? please help [Re: rumfor69]
    #26611072 - 04/18/20 05:19 PM (3 years, 9 months ago)

In my opinion looking good! Lower temperatures (within reason) don't cause any problems just slower colonisation as far as I'm aware.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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Offlineaspiringmyconoob
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Re: are my grain bags contaminated? please help [Re: rumfor69]
    #26611506 - 04/18/20 08:54 PM (3 years, 9 months ago)

Awesome, thank you so much for the quick reply and for the information. I'm glad to know that some indirect light will not hurt them because there is minimal light coming from the gap around the door frame and from the lights on the space heater. My temperature has mostly been around 74-76 degrees, do you think I should lower the temperature to the lower 70's?

I'm very glad to hear about the condensation because my heart sank when I first saw it. I think I panicked at first because I didn't realize that the temperature of the room had dropped about 5 degrees. After I got the room temperature back up, most of the condensation went away besides the remaining condensation you see in the pics. I was still worried though because of the slow recovery and because I really haven'y seen that much condensation stay after a temperature change, but you've relieved my worry.

From what you can see, how much longer do you think I should allow the grain to colonize? I'm going to mix my grain in a monotub with prepasteurized compost substrate for mushrooms I bought from an established vendor. I received the compost a week ago yesterday, and it's supposed to be good for up to 14 days at room temperature. I jumped the gun and bought the compost too early, rookie mistake. I'm guessing when I received it, it was probably already at room temperature for a day or 2. I'm guessing that I would need to mix my grain with the compost by this coming Friday for it to be good. The vendor stated that the compost could be frozen and then left to unthaw at room temperature when ready to be used. Have you ever frozen already pasteurized compost and then unthawed and used it when you needed it, or is it too risky? If my grain won't be ready by this Friday and it's too risky to unthaw and use it, then I will just order new so I have fresh compost when it's ready to be mixed with the grain. I'm not sure if the freezing could potentially dry it out some or kill the good bacteria.

Thanks again for your help and the information. I know I have asked a lot of questions, but there is so much to learn. I come up with a question, it gets answered, then I read something else that makes me question what I read or gives me new ideas. This is going to be a learning experience, but I greatly appreciate the knowledge and wisdom that I can hopefully learn from you and other experienced veterans on here.


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Offlineaspiringmyconoob
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Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26611527 - 04/18/20 09:03 PM (3 years, 9 months ago)

Awesome, thank you so much for the quick reply and information. Do you see a problem with me doing laundry while the colonizing bags remain in the room, or should I continue to place them somewhere else while I am doing laundry? I am going to place my monotub in the same laundry room, do you think the fumes and whatever is floating in the air while I do laundry could contaminate the colonizing monotub? While the tub is colonizing, I will have the holes covered with non-breathable packing tape and the lid on. From what I read, the lid is imperfect and therefore will allow enough gas exchange for colonization, but will it allow contamination to enter the tub? I've seen people place plastic wrap over the tub and then place the lid on or place the colonizing tub in a trash bag, I'm not sure if i need to do any of that or not. Again, I'm a noob and trying to learn so I apologize for my 1000 questions. Any and all help is greatly appreciated, thanks again.


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InvisibleThe Fresh PrintsS
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Re: are my grain bags contaminated? please help [Re: aspiringmyconoob]
    #26611583 - 04/18/20 09:38 PM (3 years, 9 months ago)

It wont hurt to do your laundry in the same room. The bags should be safe if the filter patch is legit.


--------------------


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Offlineaspiringmyconoob
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Re: are my grain bags contaminated? please help [Re: The Fresh Prints]
    #26611609 - 04/18/20 09:47 PM (3 years, 9 months ago)

Awesome ok good, thanks for the reply. When my monotub is colonizing, should I place plastic wrap over the top before I put the lid on or put the monotub in a trash bag while colonizing to keep out contamination and increase co2? I guess I should have stated that I was worried about the fumes because I use ammonia in my colors and bleach with my whites, both put off a good amount of chemical odor until the cycle rinses. Thanks again for the reply, all help is greatly appreciated.


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InvisibleHtaeh
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Registered: 10/14/18
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Re: are my grain bags contaminated? please help [Re: aspiringmyconoob]
    #26612001 - 04/19/20 03:24 AM (3 years, 9 months ago)

Quote:

aspiringmyconoob said:
Awesome, thank you so much for the quick reply and for the information. I'm glad to know that some indirect light will not hurt them because there is minimal light coming from the gap around the door frame and from the lights on the space heater. My temperature has mostly been around 74-76 degrees, do you think I should lower the temperature to the lower 70's?




Lower 70s is just fine. Higher temps can raise chances for contamination.

Quote:

aspiringmyconoob said:
I'm very glad to hear about the condensation because my heart sank when I first saw it. I think I panicked at first because I didn't realize that the temperature of the room had dropped about 5 degrees. After I got the room temperature back up, most of the condensation went away besides the remaining condensation you see in the pics. I was still worried though because of the slow recovery and because I really haven'y seen that much condensation stay after a temperature change, but you've relieved my worry.




Mycelium breathes, because its a living thing, hence the condensation!

Quote:

aspiringmyconoob said:
From what you can see, how much longer do you think I should allow the grain to colonize? I'm going to mix my grain in a monotub with prepasteurized compost substrate for mushrooms I bought from an established vendor. I received the compost a week ago yesterday, and it's supposed to be good for up to 14 days at room temperature. I jumped the gun and bought the compost too early, rookie mistake. I'm guessing when I received it, it was probably already at room temperature for a day or 2. I'm guessing that I would need to mix my grain with the compost by this coming Friday for it to be good. The vendor stated that the compost could be frozen and then left to unthaw at room temperature when ready to be used. Have you ever frozen already pasteurized compost and then unthawed and used it when you needed it, or is it too risky? If my grain won't be ready by this Friday and it's too risky to unthaw and use it, then I will just order new so I have fresh compost when it's ready to be mixed with the grain. I'm not sure if the freezing could potentially dry it out some or kill the good bacteria.




Grain (AFAIK) should be 100% colonised before introduced as spawn because uncolonized grain acts as a vector for contamination. I personally never set out to use a grow kit nor premade sub, I know everyone is different and yourself a beginner who probably just wants less variables to cause problems, understandably, when you first start out you want some amount of success; that being said I've read that people have experienced issues when purchasing premade sub (that's not to say you will) and making your own sub is an inevitable process you'll need to learn if you wish to continue the hobby with less expense of premade sub and a larger amount of it for multiple grows.

Quote:

aspiringmyconoob said:
Thanks again for your help and the information. I know I have asked a lot of questions, but there is so much to learn. I come up with a question, it gets answered, then I read something else that makes me question what I read or gives me new ideas. This is going to be a learning experience, but I greatly appreciate the knowledge and wisdom that I can hopefully learn from you and other experienced veterans on here.




While many will tell you (and it is a fantastic tool make no mistake) the search function at the top right hand side of the page will answer many if not all of your questions, just be sure to right the key words, select mushroom cultivation forum, newer than 4 years and check the 'one result per thread' checkbox; if you're looking for a tek hit the trusted cultivator checkbox too. However, I'm not expert by any means and can appreciate getting direct answers to my own worded questions so it doesn't bother me you asking such things because I'll always try to help those if I can. It's a huge hobby with many 'no right' answers, here experience is king. I find it really does boil down to 'just get stuck in'.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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OfflineMellowbie
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Re: are my grain bags contaminated? please help [Re: Htaeh] * 1
    #26612115 - 04/19/20 06:06 AM (3 years, 9 months ago)

Hello, I was wondering the same for mine. I will be growing all in bag. Please see below











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πŸŒ™βœ¨πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™
πŸŒ™βœ¨πŸ„πŸŒ™βœ¨πŸ„πŸŒ™βœ¨πŸ„πŸŒ™πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„


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InvisibleHtaeh
Immortal
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Registered: 10/14/18
Posts: 320
Re: are my grain bags contaminated? please help [Re: Mellowbie]
    #26612121 - 04/19/20 06:11 AM (3 years, 9 months ago)

Looks okay to me, though a second opinion from someone more experienced would be better.


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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OfflineUnknown error
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Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26612131 - 04/19/20 06:23 AM (3 years, 9 months ago)

It’s not looking too good. By the look of the uncolonised grain that kit was in a bad way before you inoculated it.


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InvisibleHtaeh
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Re: are my grain bags contaminated? please help [Re: Unknown error]
    #26612134 - 04/19/20 06:29 AM (3 years, 9 months ago)

Not a case of 'still colonising'?


--------------------
"To write is to wage war" ~ Voltaire
"The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D.
"A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher 
"We'll know our disinformation program is complete when everything the US public believes is false."  ~  William J. Casey, 1981
"Fear always works to influence the population, when the population is uneducated."
"The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~  Maximilien Robespierre
"Knowledge makes a man unfit to be a slave."  ~  Frederick Douglass


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OfflineUnknown error
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Re: are my grain bags contaminated? please help [Re: Htaeh] * 2
    #26612137 - 04/19/20 06:31 AM (3 years, 9 months ago)

It all looks very bacterial


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OfflineMellowbie
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Re: are my grain bags contaminated? please help [Re: Unknown error]
    #26612141 - 04/19/20 06:34 AM (3 years, 9 months ago)

Quote:

Unknown error said:
It all looks very bacterial





Are you referring to mine?


--------------------
πŸŒ™βœ¨πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™
πŸŒ™βœ¨πŸ„πŸŒ™βœ¨πŸ„πŸŒ™βœ¨πŸ„πŸŒ™πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„


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OfflineUnknown error
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Re: are my grain bags contaminated? please help [Re: Mellowbie] * 1
    #26612145 - 04/19/20 06:36 AM (3 years, 9 months ago)

Yeah. It’s better if you start your own threads when you’ve got a question to avoid confusion.


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OfflineMellowbie
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Re: are my grain bags contaminated? please help [Re: Unknown error]
    #26612149 - 04/19/20 06:39 AM (3 years, 9 months ago)

Quote:

Unknown error said:
Yeah. It’s better if you start your own threads when you’ve got a question to avoid confusion.




Still working on learning this forum and can’t find the β€œstart my on thread” forum... lol.. oy vey


--------------------
πŸŒ™βœ¨πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™
πŸŒ™βœ¨πŸ„πŸŒ™βœ¨πŸ„πŸŒ™βœ¨πŸ„πŸŒ™πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„


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OfflineLogicaL ChaosM
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Re: are my grain bags contaminated? please help [Re: Mellowbie] * 2
    #26612152 - 04/19/20 06:44 AM (3 years, 9 months ago)

Click this link and then click "Post" to start a new thread: https://www.shroomery.org/forums/postlist.php/Board/2

OP, you bag looks fine. The grain looks dry but seems to be colonized easily so yer all good :thumbup:


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OfflineMellowbie
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Re: are my grain bags contaminated? please help [Re: LogicaL Chaos] * 2
    #26612157 - 04/19/20 06:47 AM (3 years, 9 months ago)

Thanks!!! You rock ☝🏼


--------------------
πŸŒ™βœ¨πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™
πŸŒ™βœ¨πŸ„πŸŒ™βœ¨πŸ„πŸŒ™βœ¨πŸ„πŸŒ™πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„βœ¨πŸŒ™πŸ„


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OfflineLogicaL ChaosM
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Re: are my grain bags contaminated? please help [Re: Mellowbie] * 1
    #26612160 - 04/19/20 06:48 AM (3 years, 9 months ago)

Rock on! :headbang:


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Offlineaspiringmyconoob
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Re: are my grain bags contaminated? please help [Re: Htaeh]
    #26612769 - 04/19/20 11:34 AM (3 years, 9 months ago)

You're a life saver sir, I greatly appreciate you taking the time to answer my many questions from an anxious beginner. I will definitely keep the temperature in the lower 70's and not worry about some condensation.

I will probably wait at least another week for the grain to fully colonize then. Yeah, the more I read and research, the more I realize that I will eventually need to make my own grain and substrate. Like you said, with it being my first time, the pre made grain and substrate seemed like the easier solution this time. I want to make sure I get the process down and can even get a harvest before I attempt to learn and master the production of viable grain and substrate. It just seemed that making my own and attempting this for the time would have too many factors and potential avenues to screw up and contaminate for my first attempt. It sounds like I should probably just reorder some substrate closer to when my grain is ready just in case. I would rather spend the extra money and hopefully be safe instead of chancing it and then screwing everything up.

I really do greatly appreciate your time and information. Yeah I really need to figure this site out better. Hell it took me like an hour just to figure out a way to change my picture file extension and then reduce the file size small enough to get the pics on here. I noticed that some people have large pics that look fairly high resolution. My pics are small and the quality is greatly reduced. I'm going to have to figure out how to do it better. Also, I was not aware you could tweak the searches like that, so I will definitely do that. When I first started searching the site I had a hard time even getting around or searching things, but I learn something new everyday. Thanks again for being understanding and taking the time to answer and explain things, you're awesome.


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