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Nephedryn
Apothecary



Registered: 11/29/18
Posts: 18
Last seen: 11 months, 22 days
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Can you grow the bacteria bacillus amyloliquefaciens in hydrogaurd on a plate of agar?
#26611566 - 04/18/20 09:27 PM (3 years, 9 months ago) |
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I'm in Canada and cant get hydrogaurd anymore, any packages seem to get held at the border. I like the product and have about 20 ml's left. The thought crossed my mind that one could keep the bacteria alive by growing it out on agar, isolating the bacteria and making a liquid culture out of it for a perpetual root inoculate. If anyone has any thoughts on this experiment please leave a reply on why it would or wouldn't work. Posted in the growery but the forum seems dead, not sure where to post on the shroomery but i know a better discussion could be had here.
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Pliny_the_Elder
Stranger

Registered: 02/22/20
Posts: 13
Last seen: 2 years, 11 months
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Re: Can you grow the bacteria bacillus amyloliquefaciens in hydrogaurd on a plate of agar? [Re: Nephedryn]
#26613715 - 04/19/20 07:41 PM (3 years, 9 months ago) |
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I think it would be easier to use it as a starter like when make breading or propagating beer yeast. Make a sterile liquid solution with a food source the bacillus can eat, add you 20 ml starter, let it sit on a stir plate for a couple days.
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Solipsis
m̶a̶d̶ disappointed scientist


Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 5 months, 18 days
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Re: Can you grow the bacteria bacillus amyloliquefaciens in hydrogaurd on a plate of agar? [Re: Pliny_the_Elder]
#26619859 - 04/22/20 11:06 AM (3 years, 9 months ago) |
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Never heard of it, what does it do? sounds interesting.
I have used Bti before to grow out of certain mosquito cakes onto agar (LB medium i believe), and i have also put Beauveria spores onto agar to start propagating.  In general that stuff works i guess. But it may eventually be more about how good are you to control its development, for example spore formation of Bti.
Seems like a decent idea to put on agar first for the simple reason that you might wanna be sure that you are growing the right bacteria before using  Assuming you are able to roughly identify them anyway.
Maybe with more experience one can be more sure that you get the 'starter' you expect to get just like from yeast. IDK how well you can assume that it will just be the same, for example you cannot make fungal liquid culture without proper sterile conditions whereas you can do it with yeast (also because of massive inoculation i guess).
But aside of mass inoculation, do make sure you know what you are doing in terms of scaling up and controlling growth and a bunch more.
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