|
HouseOfRoses
Official Gay™

Registered: 02/09/18
Posts: 114
Loc: Oregon
Last seen: 10 months, 3 days
|
Why am I not seeing any growth?
#26610456 - 04/18/20 12:40 PM (3 years, 9 months ago) |
|
|
So I inoculated five bags of rye grains, two I inoculated with Honduras and thee with golden teacher. Both of syringes I had used in the past successfully... but its been since 3/25 and not even a hint of growth. Though, thankfully no signs of contam either. I have kept the room in the mid 70s, sometimes it dips down lower at night which I know is a little too cold. They used to be in a different room that is constantly in the upper 70s, but I had a situation that warranted moving them, they had been in there for probably two weeks though before I moved them. I put about 1 ML in each bag and the spot around the port looks a little dark with moisture so I'm hesitant to put in more and have too much fluid in the bags. I thought maybe there was growth in the middle I just couldn't see so i carefully shifted the bag around to get a look, nope, nadda, nothing in the middle either. What might have gone wrong? should I just try to reinoculate them...? keep them warmer for a while longer and hope for the best?
|
DezGWest7
JacQ


Registered: 04/09/20
Posts: 29
Last seen: 1 year, 8 months
|
Re: Why am I not seeing any growth? [Re: HouseOfRoses]
#26610480 - 04/18/20 12:49 PM (3 years, 9 months ago) |
|
|
So I'm newer side to mycology as well. I have a similar situation used 1 GT Syringe on Rye, only one of them has the obvious signs of life. The other two, nothing, nada. If I'm going to go by common sense, I'd say there just wasn't enough spores left over after inoculating the first. Could I be wrong and it'll show activity, its possible but I doubt leading up to 4 weeks if you see NO signs of life, then there just won't be any. Time to reinoculate. Shake Well this time, every time
|
HouseOfRoses
Official Gay™

Registered: 02/09/18
Posts: 114
Loc: Oregon
Last seen: 10 months, 3 days
|
Re: Why am I not seeing any growth? [Re: DezGWest7]
#26610492 - 04/18/20 12:53 PM (3 years, 9 months ago) |
|
|
Yeah that's what I figured. It just feels weird that its all five of them, and I used two different spore syringes. If it were all one syringe I would think oh maybe I just forgot to shake it or maybe I got ripped off and there weren't any spores actually in there, or I forgot something. And alas the torch I use for heating my needles is out of fuel so might be a minute until I get a change to refill it.
|
DezGWest7
JacQ


Registered: 04/09/20
Posts: 29
Last seen: 1 year, 8 months
|
Re: Why am I not seeing any growth? [Re: HouseOfRoses]
#26610523 - 04/18/20 01:06 PM (3 years, 9 months ago) |
|
|
It sucks, man. You waited all that time for no activity. I mean patience is necessary but not when you notice nothing going on. I look it over every 3-5 days to see if there's something i need to do. Did you get your liquid from trusted vendor ?
|
HouseOfRoses
Official Gay™

Registered: 02/09/18
Posts: 114
Loc: Oregon
Last seen: 10 months, 3 days
|
Re: Why am I not seeing any growth? [Re: DezGWest7]
#26610596 - 04/18/20 01:39 PM (3 years, 9 months ago) |
|
|
trusted on the spore traders subreddit, and the same syringes produced results in BRF jars too stangely
|
jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
|
Re: Why am I not seeing any growth? [Re: HouseOfRoses]
#26610767 - 04/18/20 02:55 PM (3 years, 9 months ago) |
|
|
one word AGAR learn it know it live it love it its a crapshoot going spore to grain anyways. agar is a sure thing for the most part. it will keep you from wasting time and grains
|
Htaeh
Immortal


Registered: 10/14/18
Posts: 320
|
Re: Why am I not seeing any growth? [Re: jcm4620]
#26610779 - 04/18/20 03:00 PM (3 years, 9 months ago) |
|
|
Could also be that the spores just didn't germinate. Also I believe the cold isn't bad for colonisation, its just slower.
-------------------- "To write is to wage war" ~ Voltaire "The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D. "A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher "We'll know our disinformation program is complete when everything the US public believes is false." ~ William J. Casey, 1981 "Fear always works to influence the population, when the population is uneducated." "The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~ Maximilien Robespierre "Knowledge makes a man unfit to be a slave." ~ Frederick Douglass
|
Hide
I Have No Bung Hole



Registered: 11/25/14
Posts: 159
Last seen: 1 year, 3 months
|
Re: Why am I not seeing any growth? [Re: Htaeh]
#26610815 - 04/18/20 03:18 PM (3 years, 9 months ago) |
|
|
I inoculated my first jars in November and had the same problem on a few. At first I figured I hadn't let the syringe cool enough after lighting, but thinking about it now it could have been that my grain was too moist. Just a guess, would wait it out though, I got life in the end but it was very very slow to colonise from the first spot of growth.
Edited by Hide (04/18/20 03:20 PM)
|
mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
|
Re: Why am I not seeing any growth? [Re: Htaeh]
#26610833 - 04/18/20 03:25 PM (3 years, 9 months ago) |
|
|
As a man whom has purchased more MS syringes then he is willing to admit, this happens quite a lot, sometimes your spores just don't germinate - when this happens I save the grain as long as there isn't any mold growth after a few weeks of realizing my spores died (or have been dead). Agar is a wonderful tool which I have really gotten into and you get WAY better results, faster growth, and less deviation in mushroom size, shape, even potency. Continuing with my sad experiences with MS syringes I have a recent one where I used a 10cc A+ syringe from a very reputable vendor with zero germination when put to grain, the syringe itself was brand new (at least to me) dated march 2020, the grain sat for two months with no germination of any kind - not even mold. After the jar didn't germinate I got pretty frustrated with MS syringes and grabbed out my B+ spores from my last grow, equipped myself with #11 blades, petri dishes, agar pyrex vessel, agar agar, Malt Extract, and even built myself a new SAB to boot. Its only been two weeks since I started my first dish and I already have isolated good fast growing genes which have now been added to the old A+ jars. Sorry for the long post! My point is Agar is the way to go hands down. Screw MS.

--------------------
Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
|
HouseOfRoses
Official Gay™

Registered: 02/09/18
Posts: 114
Loc: Oregon
Last seen: 10 months, 3 days
|
Re: Why am I not seeing any growth? [Re: mushhead]
#26610862 - 04/18/20 03:36 PM (3 years, 9 months ago) |
|
|
well I guess its a good thing I orders some agar fro the first time a couple days ago... I just need to figure out a way to put it in the bag and reseal it without contaminating anything. I've seen videos how to do it with jars, but that's obviously different
|
Htaeh
Immortal


Registered: 10/14/18
Posts: 320
|
Re: Why am I not seeing any growth? [Re: mushhead]
#26610884 - 04/18/20 03:46 PM (3 years, 9 months ago) |
|
|
No way man savage about your A+ ms, my last grow was B+ and I found an almost empty ms syringe of B+ recently and decided to begin agar. The spores did not germinate (syringe was old and not stored properly) so I bought some A+ and the syringe is packed full of spores, so far geminated some no pours successfully and tonight done about 10 transfer plates (did a few more a few days ago but it was my first time and I fucked all of them aha.. valuable experience though). So with hopes these transfers were better and I get some A+ shoe boxes running!
-------------------- "To write is to wage war" ~ Voltaire "The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D. "A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher "We'll know our disinformation program is complete when everything the US public believes is false." ~ William J. Casey, 1981 "Fear always works to influence the population, when the population is uneducated." "The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~ Maximilien Robespierre "Knowledge makes a man unfit to be a slave." ~ Frederick Douglass
|
mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
|
Re: Why am I not seeing any growth? [Re: HouseOfRoses] 1
#26610885 - 04/18/20 03:47 PM (3 years, 9 months ago) |
|
|
Quote:
HouseOfRoses said: well I guess its a good thing I orders some agar fro the first time a couple days ago... I just need to figure out a way to put it in the bag and reseal it without contaminating anything. I've seen videos how to do it with jars, but that's obviously different
Honestly, jars are the way to go.
--------------------
Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
|
CocaineBuffet
Stranger



Registered: 08/29/19
Posts: 3,456
Last seen: 33 minutes, 17 seconds
|
Re: Why am I not seeing any growth? [Re: HouseOfRoses]
#26610888 - 04/18/20 03:47 PM (3 years, 9 months ago) |
|
|
You can just cut it open, drop the clean agar into the bag and seal it up with micropore tape of just tape period. Its easier with jars if you dont have an impulse sealer.
|
mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
|
Re: Why am I not seeing any growth? [Re: Htaeh] 1
#26610890 - 04/18/20 03:48 PM (3 years, 9 months ago) |
|
|
Quote:
Htaeh said: No way man savage about your A+ ms, my last grow was B+ and I found an almost empty ms syringe of B+ recently and decided to begin agar. The spores did not germinate (syringe was old and not stored properly) so I bought some A+ and the syringe is packed full of spores, so far geminated some no pours successfully and tonight done about 10 transfer plates (did a few more a few days ago but it was my first time and I fucked all of them aha.. valuable experience though). So with hopes these transfers were better and I get some A+ shoe boxes running!
Save me some prints I'll trade you some B+ prints
--------------------
Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
|
Htaeh
Immortal


Registered: 10/14/18
Posts: 320
|
Re: Why am I not seeing any growth? [Re: mushhead]
#26610892 - 04/18/20 03:49 PM (3 years, 9 months ago) |
|
|
Absolutely good man.
-------------------- "To write is to wage war" ~ Voltaire "The Drug Medical System cannot bear examination. To explain it would be to destroy it.." ~ R.T. Trall M.D. "A single lie destroys a whole reputation for integrity." ~ Baltasar Gracian (1601-1658), Spanish philosopher "We'll know our disinformation program is complete when everything the US public believes is false." ~ William J. Casey, 1981 "Fear always works to influence the population, when the population is uneducated." "The secret of freedom lies in educating people, the secret of tyranny is in keeping them ignorant." ~ Maximilien Robespierre "Knowledge makes a man unfit to be a slave." ~ Frederick Douglass
|
|