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Offlineeagle12
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Registered: 04/11/19
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Last seen: 3 years, 6 months
When do you like to cut transfers for agar to agar?
    #26605820 - 04/16/20 12:58 PM (3 years, 9 months ago)

Would like to get this thread going. I like to wait about a week after i see myc before i do any transfers...what are your thoughts?


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OfflineGrowtech
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Registered: 11/09/19
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Last seen: 2 years, 5 months
Re: When do you like to cut transfers for agar to agar? [Re: eagle12]
    #26605845 - 04/16/20 01:11 PM (3 years, 9 months ago)

For me, the earlier the better... if you are doing a transfer to get rid of contamination, let it grow a bit away from the contamination and take a transfer from the leading edge of growth.  I don't think there's a specific time frame so much as an actual growth amount (of course they are loosely tied together).  Taking a transfer when the leading edge is halfway between center of petri and edge pf petri seems about right to me (assuming you are using a petri and they are 100mm dishes).


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Mycolo.gyGenetics & yield tracking system


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Offlinepoisoned
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Registered: 04/17/13
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Re: When do you like to cut transfers for agar to agar? [Re: Growtech]
    #26605925 - 04/16/20 01:52 PM (3 years, 9 months ago)

That's quite a lot. I use 80mm dishes and I usually noc them 3 times. Don't even have the room to let them grow to 50mm.

Actually, I'm adding some 60mm glass dishes to my inventory soon. I loved the plastic 60mm ones.


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How I do glass dishes


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OfflineGrowtech
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Re: When do you like to cut transfers for agar to agar? [Re: poisoned]
    #26605934 - 04/16/20 01:55 PM (3 years, 9 months ago)

What are you doing the transfers for?  If it's to get rid of contams, that's different than trying to isolate a single strain.  Basically transfer earlier rather than later, but also trying to not include any possible contams.


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Mycolo.gyGenetics & yield tracking system


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Offlinepoisoned
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Re: When do you like to cut transfers for agar to agar? [Re: Growtech]
    #26605939 - 04/16/20 01:57 PM (3 years, 9 months ago)

It depends entirely on contam I'm dealing with. If there's a mold in there, I better do a transfer before it sporulates. If there was a sporulated mold in my previous transfer, I'll also do another transfer as soon as possible.

For bacteria, I let it grow out a bit more. But not to 50mm.


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How I do glass dishes


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OfflineGrowtech
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Re: When do you like to cut transfers for agar to agar? [Re: poisoned]
    #26605949 - 04/16/20 02:00 PM (3 years, 9 months ago)

Just for clarification, I never meant 50mm... I said from the center to halfway to the edge on a 100mm plate which is 25mm.  :smile:  50mm of growth before a transfer is silly.


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Mycolo.gyGenetics & yield tracking system


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Offlineeagle12
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Re: When do you like to cut transfers for agar to agar? [Re: Growtech]
    #26606575 - 04/16/20 07:55 PM (3 years, 9 months ago)

Might have cut a little early most was dime size growth and i took 3.cuts


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OfflineSockadin
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Re: When do you like to cut transfers for agar to agar? [Re: eagle12]
    #26606583 - 04/16/20 08:00 PM (3 years, 9 months ago)

I think maybe we are all over thinking the question.
Are you starting from a spore swipe?

Cut out a few sections and isolate the organized growth.

If your running away from contams re think your sterile procedure.


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Offlineeagle12
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Re: When do you like to cut transfers for agar to agar? [Re: Sockadin]
    #26609376 - 04/18/20 12:11 AM (3 years, 9 months ago)

Yes spore swipe


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Invisiblec10h12n2o
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Re: When do you like to cut transfers for agar to agar? [Re: eagle12]
    #26609514 - 04/18/20 03:22 AM (3 years, 9 months ago)

For spores I like to use the streaking technique in my sig (covered in detail In my agar guide)

This makes it extremely easy to get a clean culture in very few transfers, especially if you transfer from later zones

I prefer to transfer colonies from the latest zone that germinates, basically as soon as it is visible, or around the size of a match head maximum.  Will often put 2 or 3 of these colony transfers onto the t1 plate, then transfer from those before they intersect to individual t2 plates, which are almost always safe to use for ms grows at that point



Here are some examples of what it looks like in practice:



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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (04/18/20 03:28 AM)


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Offlinemetaphoric
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Re: When do you like to cut transfers for agar to agar? [Re: c10h12n2o]
    #26609532 - 04/18/20 03:43 AM (3 years, 9 months ago)

Lately I've been really into the poke method. Takes a couple extra days maybe but it's so simple and pretty.

If you're not familiar with the poke method you just take a sterile needle, poke some clean growth, then poke your new plate. It will look like you haven't transferred anything at all but in a couple days you'll see myc growing out of your microscopic transfer.

IMO it is great for both eliminating contams and creating more uniform genetics. I'll usually PC 5 vendor needles in a tall ziploc then use each needle for 2 transfers. No flame. 1 plate becomes 10.


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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OfflineSYF8
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Re: When do you like to cut transfers for agar to agar? [Re: metaphoric]
    #26610098 - 04/18/20 10:28 AM (3 years, 9 months ago)

Quote:

metaphoric said:
Lately I've been really into the poke method. Takes a couple extra days maybe but it's so simple and pretty.

If you're not familiar with the poke method you just take a sterile needle, poke some clean growth, then poke your new plate. It will look like you haven't transferred anything at all but in a couple days you'll see myc growing out of your microscopic transfer.

IMO it is great for both eliminating contams and creating more uniform genetics. I'll usually PC 5 vendor needles in a tall ziploc then use each needle for 2 transfers. No flame. 1 plate becomes 10.




I’m familiar now, thanks.  I’ll do some traditional transfers and some poke transfers with my next plates that grow out and see what happens.


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Offlinemetaphoric
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Registered: 05/18/15
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Re: When do you like to cut transfers for agar to agar? [Re: SYF8]
    #26610424 - 04/18/20 12:26 PM (3 years, 9 months ago)

I'm still a bit of a noob on agar so try that and also the traditional scalpel methods too to figure out what you like.

I think the guy who wrote the poke tek was named Josex. You should try finding that so you can read it all for yourself.


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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