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tomcards



Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
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Storing Parafilmed plates and slants in sealed or unsealed ziplock bags?
#26607442 - 04/17/20 08:03 AM (3 years, 9 months ago) |
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Guys,
I'm beginning to work with agar for the first time. I'll wrap my inoculated plates and slants with Parafilm. For storage, I've read that inoculated plates and slants wrapped with Parafilm should be placed in a Ziplock bag in the refrigerator.
But, is the Ziplock bag closed or open? I know that closed Ziplock bags allow for some GE. But it seems that if the plate or slant is already wrapped with Parafilm, putting it inside a closed Ziplock bag might suffocate the mycelium.
So which is it? Open or closed Ziplock bag to store Parafilmed plates and slants. Please advise.
Thanks,
Tom
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Ombisha
Transmutant



Registered: 02/17/20
Posts: 667
Loc: Tartaria
Last seen: 1 year, 3 months
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Re: Storing Parafilmed plates and slants in sealed or unsealed ziplock bags? [Re: tomcards]
#26607492 - 04/17/20 08:27 AM (3 years, 9 months ago) |
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i store sealed plates in two sealed bags... but i only started working with agar a few months ago, so i am gonna tag along in this thread. Maybe i will learn something
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Mycoactive
Scientist


Registered: 11/20/19
Posts: 185
Last seen: 27 days, 42 minutes
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Re: Storing Parafilmed plates and slants in sealed or unsealed ziplock bags? [Re: Ombisha]
#26607529 - 04/17/20 08:53 AM (3 years, 9 months ago) |
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If you're putting them in the fridge, most species won't consume much oxygen. If you have a very clean fridge, no bag is necessary. If it's the same fridge where you store food, then the bag can help keep the outside surfaces clean. They should be totally fine in a closed ziplock bag.
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fungi4us
Stranger
Registered: 09/28/19
Posts: 19
Last seen: 1 year, 9 months
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Re: Storing Parafilmed plates and slants in sealed or unsealed ziplock bags? [Re: tomcards]
#26607613 - 04/17/20 09:22 AM (3 years, 9 months ago) |
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the bag is kinda like a condom you only use them when necessary. #1 being in a dirty home but as long as you do normal cleaning daily then you should be ok like when suge night got out everyone was safe riiiiiiiiggghttttt....lol
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tomcards



Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
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Re: Storing Parafilmed plates and slants in sealed or unsealed ziplock bags? [Re: fungi4us]
#26607655 - 04/17/20 09:41 AM (3 years, 9 months ago) |
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Thanks guys. Yes, the plates and slants will be in my food fridge. So, I will close the bags. I'll stack two Parafilmed plates in each sealed bag.
Last question. Can I store several slants in each closed bag, or should I store one slant in each bag?
Thanks.
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Mycoactive
Scientist


Registered: 11/20/19
Posts: 185
Last seen: 27 days, 42 minutes
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Re: Storing Parafilmed plates and slants in sealed or unsealed ziplock bags? [Re: tomcards]
#26607693 - 04/17/20 09:52 AM (3 years, 9 months ago) |
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Quote:
tomcards said: Thanks guys. Yes, the plates and slants will be in my food fridge. So, I will close the bags. I'll stack two Parafilmed plates in each sealed bag.
Last question. Can I store several slants in each closed bag, or should I store one slant in each bag?
Thanks.
Several in the same bag is fine.
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tomcards



Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
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Re: Storing Parafilmed plates and slants in sealed or unsealed ziplock bags? [Re: Mycoactive]
#26607789 - 04/17/20 10:28 AM (3 years, 9 months ago) |
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Thank you, Mycoactive!
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
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Re: Storing Parafilmed plates and slants in sealed or unsealed ziplock bags? [Re: Mycoactive]
#26607796 - 04/17/20 10:29 AM (3 years, 9 months ago) |
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Slants in fridge makes sense, you want them to grow as little as possible.
Inoculated plates should be stored at room temp, because you want them to grow.
As far as ziplocks, I use individual ziplock sandwich bags for each plate instead of wrapping the plates.
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tomcards



Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
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Re: Storing Parafilmed plates and slants in sealed or unsealed ziplock bags? [Re: LadysKnight]
#26607824 - 04/17/20 10:37 AM (3 years, 9 months ago) |
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Yes, I know that inoculated plates should be grown at room temperature.
I was referring to storing inoculated master plates (whose growth I want to inhibit) in the refrigerator.
Thanks for clarifying that point for others.
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