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b2b
b2b
Registered: 08/09/12
Posts: 18
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Dry tissue rehydration in peroxide and bleach
#26606547 - 04/16/20 07:44 PM (3 years, 9 months ago) |
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I am testing various ways of cloning dry mushroom tissue, but without much success due to immediate contamination.
Tried various combinations using agar plates: - rehydrating in 3% peroxide - 10% bleach - dry tissue - used mush cap and stem samples
I am using laminar hood and always test the agar plates by keeping one plate empty. So I know it is not the environment, but the tissue itself.
Contamination develops with every method I tried, usually within 2 days.
The only thing I did not try is using antibiotic agar.
If you have experience with this, I would love to hear your thoughts.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
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Re: Dry tissue rehydration in peroxide and bleach [Re: b2b]
#26606573 - 04/16/20 07:54 PM (3 years, 9 months ago) |
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Mushrooms are fruited in open air and dehydrated in an even more open environment. Your gonna get healthy mold spores that will always outrun your dry culture.
I have tried this it doesn't work. Sorry to break the bad news.
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Ombisha
Transmutant



Registered: 02/17/20
Posts: 667
Loc: Tartaria
Last seen: 1 year, 4 months
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Re: Dry tissue rehydration in peroxide and bleach [Re: Sockadin]
#26607164 - 04/17/20 03:01 AM (3 years, 9 months ago) |
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Oh, really? I have been planning to try cloning p. serbica this way... Is it widely accepted that "dry cloning" doesn't work? I thought that cronicr had some success with it?
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metaphoric
Mr. Bater



Registered: 05/18/15
Posts: 730
Last seen: 2 years, 3 months
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Re: Dry tissue rehydration in peroxide and bleach [Re: Ombisha] 1
#26607186 - 04/17/20 03:45 AM (3 years, 9 months ago) |
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Do you rip it open and pull a tiny smile from the inside? After washing the outside with alcohol
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 BEHOLD 
        
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Dry tissue rehydration in peroxide and bleach [Re: Ombisha]
#26607221 - 04/17/20 04:54 AM (3 years, 9 months ago) |
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Quote:
Ombisha said: Oh, really? I have been planning to try cloning p. serbica this way... Is it widely accepted that "dry cloning" doesn't work? I thought that cronicr had some success with it?
Exactly one person ever maybe. I'm sure more have done it but it's absolutely not worth the time unless it's the last sample of a near extinct species.
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
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Re: Dry tissue rehydration in peroxide and bleach [Re: bodhisatta]
#26607251 - 04/17/20 05:26 AM (3 years, 9 months ago) |
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Or if you're just having some time on your hand and want to have some fun.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Dry tissue rehydration in peroxide and bleach [Re: poisoned]
#26607307 - 04/17/20 06:40 AM (3 years, 9 months ago) |
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I suppose. But I'd rather use all my time and supplies making things that work and are a good use of the time and resources
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