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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26478310 - 02/10/20 10:29 AM (4 years, 7 days ago)

Nice!


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26492858 - 02/19/20 05:50 AM (3 years, 11 months ago)

Two questions:

1) If no pours are thicker, and thicker plates promote slower, less ropey growth, should one mix up less nute agar to keep ropey growth? (in my case thick poured petris)

2) When blending LI... is fluffy or ropey more frail to the blender blades?


(my apologies, always forget how to spell technical terms for ropey and fluffy)


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26493018 - 02/19/20 08:28 AM (3 years, 11 months ago)

It’s the same for me whether mycelia is tomentose or rhizo.  I don’t give that much thought just blend and pour.


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OfflineA.k.aM
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26493094 - 02/19/20 09:23 AM (3 years, 11 months ago)

No pours are however thick you make them right


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
    #26493136 - 02/19/20 10:00 AM (3 years, 11 months ago)

Quote:

A.k.a said:
No pours are however thick you make them right




I don't make them... (I pour thick plates for LI) But I was reading that thinner plates can help push mycelia into rhizo growth...


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26493643 - 02/19/20 03:56 PM (3 years, 11 months ago)

So by no pour do you mean inject with a syringe?


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26493768 - 02/19/20 05:27 PM (3 years, 11 months ago)

I'm trying to get to the best method of making thick petri dishes for Blending to LI. The last dishes I made were thick and soft and tomentose... and I was wondering if I grow some cubes out on thin hard dishes, get them ropey.... how I might keep them ropey on my thick plates. (I like them thick so there is a ton of agar in my syringe)

I like them thick.


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Clean spawn thread | Put a thermometer on your PC


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InvisiblefahtsterM
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26493930 - 02/19/20 06:55 PM (3 years, 11 months ago)

That’s what she said


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: fahtster] * 1
    #26493970 - 02/19/20 07:23 PM (3 years, 11 months ago)

Aa+, rw, wg are all tomentose. Ive never seen a rhizo with any of these and they perform well. 6-7z dry per flush average.


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26493988 - 02/19/20 07:40 PM (3 years, 11 months ago)

Thats cool to know. I've have speedier grows when my GT are ropey.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26494926 - 02/20/20 10:57 AM (3 years, 11 months ago)

Myc is myc right it’s all getting busted up in the end anyhow.

Dude no joke about aa+ being tomentose, it took three transfers off the germ plate until it wasn’t just a big cotton ball looking mess. I do finally have a nice rhizo plate of it going now though.

I was really impressed with how effective li is, I just hit up my first jar with it last week and it took a while to recover but when it did the whole jar blew up.


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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
    #26497974 - 02/22/20 05:06 AM (3 years, 11 months ago)



AA+ slurryinahurry tek. First time ever fruiting cubes in a mono.




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Germinating old spores progress page
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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: spiritlands]
    #26498077 - 02/22/20 07:27 AM (3 years, 11 months ago)

Nice job! You got a top layer on those? They look a little dry.


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OfflineMycQueen
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26515279 - 03/03/20 07:09 PM (3 years, 11 months ago)

I have several good clean varieties on agar that I want to blend & inoculate into WBS grain jars in one mega session, but I only have one blender blade assembly :frown:.
Which of the below is best practice or acceptable (if none of the below or you have a better idea, please share);
A] Use several jars of sterile water with bleach or IP alcohol added after PCing, as a sanitizing blade rinse between each variety? (what % to use?)
B] Re wrap in foil & PC blade assembly between each variety? (how long?)
C] Buy one blade for each variety? (know where I can get a deal on a dozen oster blades/seals ASAP?)
Thanks


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: MycQueen] * 1
    #26515323 - 03/03/20 07:29 PM (3 years, 11 months ago)

Buy several blade assemblies at Goodman’s. Sterilize everything and do it all in one session.

You can rewrap the blade assembly and pc again. I go 30 minutes at 15 psi. But always get backup blades and pour lids.


http://www.goodmans.net/i/2589/ice-crusher-blender-blade-cutter-for-oster-osteriz.htm

Walmart also has a replacement pack that has blade assembly, gasket and the black plastic base for 10$. Check local stock online before going.

https://www.walmart.com/ip/Oster-Ice-Blade-004961-011-NP0/22967482?wmlspartner=wlpa&selectedSellerId=83&adid=22222222228016405543&wl0=&wl1=g&wl2=m&wl3=69708034976&wl4=pla-116893132856&wl5=9012027&wl6=&wl7=&wl8=&wl9=pla&wl10=8175035&wl11=online&wl12=22967482&veh=sem&gclid=EAIaIQobChMI_e79yOP_5wIVCNbACh1xBgg5EAQYASABEgInNvD_BwE



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OfflineMycQueen
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26515385 - 03/03/20 08:01 PM (3 years, 11 months ago)

Thanks Eat, you're the best!


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Re: EAT LIQUID INOCULATES™ [Re: MycQueen]
    #26515425 - 03/03/20 08:21 PM (3 years, 11 months ago)

OMG how far this idea has come. This is a beautiful write up. Can't believe it has taken me this long to read it... I remember back in the day when we chatted about the idea and I tried to do one but had to abandon the concept because I moved and didn't have time to continue cultivating.

Nicely done!

:thumbup:


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26523535 - 03/08/20 08:26 AM (3 years, 11 months ago)

Quote:

3) Now place the grey gasket down over the silicone and press down to allow the gasket to set in. The gasket is necessary so that the jar seals properly when you are blending the jar. Otherwise it will explode or leak out of the jar during blending.
https://www.shroomery.org/forums/thumbs/15-002/095426404-thumb_SLURRYLID1.jpg




That looks like a wide mouth blender lid set up on the right (I love wide mouth jars). I know a regular mouth jar fits an Oster, but which blender fits a wide mouth jar?


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Re: EAT LIQUID INOCULATES™ [Re: MycQueen]
    #26523570 - 03/08/20 08:45 AM (3 years, 11 months ago)

You can also get regular mouth jelly jars. They work well with the oyster


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26606497 - 04/16/20 07:12 PM (3 years, 9 months ago)

Eats~

I've been having variable leap off with my LI, then I had an amazing batch that colonized an entire 4 quart bag of wheat in 5 days. But I seem to have slower colonization with dishes that race across the agar. I'm thinking that its very helpful if the agar gets colonized below the surface. I'm at 2 parts LME to 3 parts agar, made a bit soft, this makes it race and the plates look pretty, but maybe I should up the sugar to slow the growth and maybe get it deeper in the agar?


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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