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OfflineRivendell
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Registered: 03/29/20
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My First Grow Journal * 1
    #26606256 - 04/16/20 05:23 PM (3 years, 9 months ago)

Have decided to make good use of my stay at home time and pick up a hobby I've long had interest in. 

Started a journal of my first build and grow.    Wish me luck! (Also seems the journal posts to community, so I thought I'd cross thread over here in Cultivation)  Hope that's cool..
https://www.shroomery.org/forums/showflat.php/Number/26606220

Starting up a grow..

1st time actively cultivating, but lifelong science nerd..that's quarantined in Colorado.  So why not?

I've done a ton of lurking in the boards and FB groups, read up on a million teks and thought I'd give it my own go.

Still early in the build process (photos attached).  Using "The Mushroom Cultivator:A Practical Guide to Growing Mushrooms at Home" by Stamets and Chilton as a field guide.

I've got a SAB box w/ HEPA + dual fans (plan is to give agar a go...with some bird seed jars from a vendor and sealed while I'm doing transfers)

Planning on working with BODs Agar Tek https://www.shroomery.org/forums/showflat.php/Number/21922023

Also have a temp/humidity controlled fruiting chamber (that I'll still need to dial in) with with a lower water box/fogger for moisture control.  Fruiting chamber has seedling heating pads on the bottom and rear of the chamber with reflective backing.  Trays for spawn + casing mix.  1 big one currently, may looking into 3 down the road.. we'll see.

Plan on doing BODs Bucket Tek https://www.shroomery.org/forums/showflat.php/Number/24077162

Jiffy Mix Casing
https://www.shroomery.org/forums/showflat.php/Number/18880716#18880716

Thoughts?  Gotcha's for a rook?

Day 1 Pics










Edited by Rivendell (04/16/20 05:48 PM)


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OfflineRivendell
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Registered: 03/29/20
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26606340 - 04/16/20 05:55 PM (3 years, 9 months ago)

Day 2 Pics..

Still a work in progress, need to do some sterlization and cleaning still. 

But thought I'd post some more viewable pics of the setup in the daytime.

Full Rig on a shelving rack


Lower Water Chamber w/ Fogger


Humidity/Temperature controlled Fruiting Chamber w/ Probes


Lower Water Chamber & Fruiting Chamber - PVC connected


SAB Box


SAB HEPA Filter/Dual Fans


Edited by Rivendell (04/16/20 06:32 PM)


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OfflineJimbo DeMan
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Re: My First Grow Journal [Re: Rivendell]
    #26606376 - 04/16/20 06:09 PM (3 years, 9 months ago)

Hey, Dude, Hello there. I just started doing this also. Having one hell of a time trying to use this site. How do you post pics ? Tried talking to people but got no replies. Must be doing something wrong. Then had a problem with my password. I'm on a temp password now. Really frustrated with it all.


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Offlinebigmanmcbig
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Re: My First Grow Journal [Re: Jimbo DeMan]
    #26606398 - 04/16/20 06:19 PM (3 years, 9 months ago)

You have to post pictures to a picture file you have associated with your account you may need to make a new one. Once you upload the picture you get a link and that link becomes the picture in any new post you make. Just copy and paste the link which they give to you immediately after you upload a picture.


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OfflineJimbo DeMan
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Re: My First Grow Journal [Re: bigmanmcbig]
    #26607463 - 04/17/20 08:13 AM (3 years, 9 months ago)

Wow, a response,  thanks. Problem is I cant figure how to copy and paste, tried several times. Not used to doing it. This fellow Rivendell is setting up some high end stuff. Not me, just a small bucket grow. Any way, glad you responded. How do you copy and paste ? As I mentioned, and have seen others mention, kinda hard posting on the site. Kool ass hobby especially for those looking for somekind of therapy you cant get from a doctor and get cheap. I've been reading about anxiety and microdosing.


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Offlinebigmanmcbig
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Re: My First Grow Journal [Re: Jimbo DeMan]
    #26607542 - 04/17/20 08:59 AM (3 years, 9 months ago)

Once you upload the picture to your files it immediately takes you to a page where the images have their respective links. you just click on them and it copies it for you or you just copy it. simple as highlight and control + c to copy then control + v to paste to a post.


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OfflineJimbo DeMan
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Re: My First Grow Journal [Re: bigmanmcbig]
    #26608659 - 04/17/20 05:32 PM (3 years, 9 months ago)

Thanks Bud, I'll try it later on. I clicked to add you to an address book and I dont even see an icon for it. Kool site but a little hard to get the hang of. I did start a journal and posted it if you care to read it. I'll work on it more this weekend and try to post some pics. Wish it was as easy for me as Facebook. Peace.


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OfflineRivendell
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Re: My First Grow Journal [Re: Jimbo DeMan] * 1
    #26610506 - 04/18/20 12:58 PM (3 years, 9 months ago)

So while I'm getting my agar stuff going, I thought I'd throw some Multispore to Grain and start working on my sterile techniques.

Started off by cleaning everything externally before placing into SAB.  Have a gallon of 91% iso that I've cut down, 500ml iso + 150ml water.  Put the iso into a small bowl for usage (glass blown piece from a class two months ago seemed good as any) and soaked up with folded piece of paper towel to clean.


Placed everything inside SAB and turned on for 10 mins prior to use.


Put on lab coat and nitrile gloves to begin work in the SAB.  Wiped everything down placed into SAB with iso again.  Cut vacuum sealed pre-order bird seed jar and put needle onto 2 test MS needles, Golden Teacher & Pioppino, wiped each needle down, gently shook needle and each jar and shot a couple of drops into each bird seed container to begin colonizing.



Moved Colonized Bird Seed jars into fruiting chamber to spawn and dialed in temp/humidity sensors.



Checked after an hour and the area outside temp was a little cool, so I've wrapped the area up in painters plastic to keep temps warmer in grow area.  This is just going to be a temporary fix as I have a ez-up greenhouse coming in a few days with rollup windows/door.




Will check later today on how the jars are looking after 24 hrs. 

Going to get the agar going today and PC it, then pour into petri so I can start isolating genetics, but thought I'd see how this goes in the meantime.

Thoughts?  Improvements?  Advise for a 1st time grower?


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26610511 - 04/18/20 01:01 PM (3 years, 9 months ago)

By the way.. is there anyway to post short vid clips that meet the size requirements?  I have a few small videos of the misting in the fruiting chamber that I thought was cool and wanted to post.. but don't immediately see how.  Thanks fam!


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Invisiblealaskappalachian
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Re: My First Grow Journal [Re: Rivendell]
    #26610560 - 04/18/20 01:24 PM (3 years, 9 months ago)

First off- welcome to the Shroomery!  GT is a good variety to start with.  Never grown Pioppino.  Look forward to seeing what you turn out.  Good you didn't put all your eggs in one basket using all your MS solution on a bunch of grain jars (as spore syringe to grain is always a gamble, although that doen't mean those won't colonize just fine!).  Never seen a SAB with fans and a HEPA.  Moving air in a SAB is the opposite of what you are trying to achieve and instead of scrub-and-evacuate I feel like it's going to be more churn-and-contaminate.  I dunno, but just wanted to offer that up to save you some headache when you  work with agar; which brings me to that.  Bod's agar tek is perfect.  No pours are rad too.  Definately delve into agar ASAP.  You don't need a humidifier for Cubes, but I have no idea what Pioppinos like...  Good to have a setup ready for more complex myco work donw the road though so you'll be ahead of hte curve.  Also (sidenote): love that bowl- nice job.  If I had any sage advice it would be to ditch buying premade jars and such and just read up on how to make your own spawn (crazy simple) and grab some kind of grain and coir and go nuts.  Advice #2 is don't overthinkn it.  Make clean spawn, learn proper field capacity for your sub, and maintain good surface conditions, (much easier than people think once it clicks with you) and grow, grow, grow.  Practice makes pounds.  Thanks for sharing!  Look forward to seeing updates. :highfive1:


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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OfflineRivendell
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Re: My First Grow Journal [Re: alaskappalachian]
    #26610654 - 04/18/20 02:08 PM (3 years, 9 months ago)

Thanks for the feedback!

Just a couple of notes.. These units were bought from a commercial gourmet farmer (oysters, etc) that is cranking these units out.  The SAB is a positive push (will dig up the link later) that pushes air down into the box and out the holes.  Similar to the idea of a flow hood, but in a SAB. 

All of the spores are from Sporeworks, so I'm sure they'll all be solid. 

As I mentioned at the top, I'm just getting my setup going.. and wanted to get something started right away.  That's why I bought the pre-mades.  I have an All American PC 21.5 that will get showcased soon. 

Later today, I'm going to pull together some agar and PC, then do a pour tek in the SAB.  From there, I'll start isolating the cultures.. I have GT, B+, APE, Melmac (also Golden Oysters) and doing transfers.  But I wanted to get something going so I can practice the coir bucket tek/jiffy mix casing techniques.  I have an igloo cooler for that coir sterlization/transfer I'll post pics of when we get there.


Edited by Rivendell (04/18/20 02:11 PM)


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26611171 - 04/18/20 06:02 PM (3 years, 9 months ago)

Looked around for awhile and found some good recommendations for this setup. Seemed to offer the best of both worlds (SAB/FH) so for a beginner it seemed like a no brainer.

Specs from vendor -

Description

This item will not ship until April 27 due to the coronavirus slow down.

Add ons: 6 watt Uv light

Work 4 times faster than with a glove box!

The TerraFirma flow box is specifically designed for those mycologists that do not want to wear gloves, want to save time or regularly use bulk substrate bags or trays for their mushroom growing. This design gives the user more room, easy access and also allows you to work with out the restriction of gloves.

2 large 5200 RPM fans move air through the HEPA filter into the box and the air is forced out through two hand entry ports ( like a “flow hood” ). This high airflow does not allow air from the outside with algae spores to enter the box while in use.

Under the lid is a large HEPA filter, which stands for High Efficiency Particulate Air, is a designation used to describe filters that are able to trap 99.97 percent of particles that are 0.3 microns. This easy-to-use glove box creates an clean environment for your mushroom cloning, inoculation and spawning tasks. If used correctly greatly reduces the chance of contaminated product. Often used as an inoculation chamber for inoculating sterilized grain, cloning, or adding grain spawn to your grow bags or trays for the home grower. The unit then can then be discreetly stored and moved without hassle.



(Standard configuration) Included in each unit is one fully assembled HEPA Flow box chamber,

(1) Plastic Storage container 24L x 18W x 15 1/4H 80Q 76L

(2) fans

(2) power supplies

(2) ABS rings.

(2) ABS plugs

(1) True 12' x 14' "high flow" True HEPA filter.


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26611352 - 04/18/20 07:29 PM (3 years, 9 months ago)

Having a real go with the temps, but I think I finally have it figured out.

Over the evening, the heating pads/reflectors didn't seem to do much good.. temp was 69.4 when I woke up this AM and checked :shocked:

I immediately grabbed a space heater and have the environment stabilized in the low 80s.



Last I checked the temp was at 82.2 (Seems to now be at 82.5)


Temps Based on Mushroom Cultivator




Edited by Rivendell (04/18/20 07:30 PM)


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Offlinebigmanmcbig
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Re: My First Grow Journal [Re: Rivendell]
    #26611542 - 04/18/20 09:09 PM (3 years, 9 months ago)

I like the set up. The only  thing i would suggest (and somebody might have already so sorry if they did) is that I also did a similar set up with the translucent garbage plastic bag walls. Be careful that you dont restrict airflow with the walls put up, not a big deal just watch it when the time comes. Other than that you're golden and will work perfect.


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Offlinejcm4620
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Re: My First Grow Journal [Re: Rivendell]
    #26611618 - 04/18/20 09:50 PM (3 years, 9 months ago)

you fell for pretty and shiny things combined with advertising my friend 😃 you will laugh at yourself later like we all did when we over thought and over engineered useless shit just cuz it looked cool and fancy. we all spent $ on crap only to let it end up collecting dust on the garage floor. if your growing cubes all of that shit is gnna be counter productive. it will cause you more problems and take up more of your time fucking with it. i just wish you would have came here and read shit first. we could have saved you a lot of $$. my advice first thing is to take a baseball bat or a shotgun and destroy that horrible thing of a non still air contamination station. as stated that is the complete opposite  of a STILL AIR BOX hence the term still air. its not a positive push particle accelerator & penetration and non sterile contamination imitation combination flow hood thingy ma jig. a flow hood is something totally different and you prolly could have bought one after all that. but all you need for cubes is a plastic tote and the know how that you will get by reading on here. i dont even wanna know how much you got taken for on all that stuff. but im willing to wager everything i have that it was way way more than you needed to spend. just chalk it up to a lesson learned. but a plain ol monotub is all you need to fruit an ungodly amount of mushrooms. now im not sayin all that shit might not be horrible for more exotic species. you wont know til you try. but you will be very shocked to see how un technical cultivation really is when you have the foundation which is clean spawn. we have all been there and wasted time and $ so dont feel bad. and dont take this as an insult in any way shape or form. some ppl are completely butt hurt & cry babies when someone simply tries to tell them the truth based on their own past mistakes. but if you dont destroy that contam box now you will later when you see how it does the opposite of what you want it to do. a pressure cooker and a dehydrator are the only somewhat big investments needed. everything else is cheap to get. i wish you nothing but success man. and maybe one day you can try to fruit some exotics in those which i would like to see. but if you take anything i say seriously plz build an actual still air box. it is essential to obtaining clean spawn. and always remember clean spawn is the most important thing. hell with clean spawn you can fruit in a dirty empty toilet bowl.😃


--------------------
PANAEOLUS FRUITING MADE SIMPLE



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OfflineRivendell
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Re: My First Grow Journal [Re: jcm4620]
    #26612153 - 04/19/20 06:44 AM (3 years, 9 months ago)

lol..certainly not going to destroy it!

We'll see how it works out.  If the positive pressure doesn't end up keeping thing sterile, then all I have to do is not turn on the fans.. and voila.  Still A SAB. 

Seems to have a lot of good reviews though from a couple of hundred people purchasing and its the 3rd generation of his boxes. Either way, I'm not sweating losing a few bucks over it.  Not exactly breaking the bank.

And I do have an All American PC as well.  Just haven't gotten to that yet.  Hope to do some agar work today and will have to PC it before I pour in the SAB. 

These jars are just my very first attempt, while I get the other stuff going.  (Also have a few hundred hours of Tek reading in Shroomery Forums.  If you look to the top I'm using specific teks for my processes)


Edited by Rivendell (04/19/20 06:46 AM)


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Offlineredhandmat
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Re: My First Grow Journal [Re: Rivendell]
    #26612171 - 04/19/20 06:55 AM (3 years, 9 months ago)

Hey! you're setup looks visually cool. But I was just wondering, why didnt you just make a flow hood if you could invest all this? Sorry if you already answered this in the thread. Im a noob myself, but it seems like many of the experienced growers that grow things in this forum have simple setups. PC is a must and then the most advanced have a flow hood. Especially with the newer mono and shoebox teks. It feels like keeping it simple still leads to amazing results.


Edited by redhandmat (04/19/20 06:56 AM)


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OnlineLogicaL ChaosM
Ascension Energy & Alien UFOs
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26612175 - 04/19/20 06:57 AM (3 years, 9 months ago)

Damn, looks like a High-Tech Myco Lab! :eek:

Cool set-up :popcorn:


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OfflineSockadin
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Re: My First Grow Journal [Re: redhandmat]
    #26612179 - 04/19/20 07:02 AM (3 years, 9 months ago)

Op I think you got ripped off on the SAB with a dual fan.

Still Air Box- this is the name of it.

You do not want air flow in your chamber that your working on clean cultures on.

I think all that electronic stuff is cool, but everything you have is useless all you need is a plastic box and a pressure cooker for this hobby.

Items required: Pressure Cooker
Plastic tote for a SAB
Shoe boxes  for fruiting
Large Quart JARS FOR grain
Small no pour Petros
Grain
Coir


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OfflineRivendell
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Re: My First Grow Journal [Re: Sockadin]
    #26612227 - 04/19/20 07:26 AM (3 years, 9 months ago)

We'll see I guess.  Like I said this is my first go at it.  Some of the items like the temp/humidity controlled fruiting chamber is so I don't have to constantly go down and mist and can keep a controlled environment.  The SAB is still a SAB without the fans going.  As mentioned, we'll give it a few go's on sterile work and see what happens.  I hear what everyone is saying, but I also have read up on this too.  As for why a SAB vs just going FB.. part of it was that I had not fully read up on the Flow Box designs prior to purchasing my setup.  If it doesn't work out, then I'll replace it.

And this setup is for more than just cubes.. plan on starting a small farmers market level business with gourmet mushies as well.  This is just a side fun project for myself.  Interested in the fruits of course, but part of the fun for me is the locking down of the science of it all.


Edited by Rivendell (04/19/20 07:32 AM)


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Offlinejcm4620
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Re: My First Grow Journal [Re: Sockadin]
    #26612258 - 04/19/20 07:45 AM (3 years, 9 months ago)

also get your terminology down. it is very important when describing things to others that you get this correct. it helps to prevent others from seeing it and picking up bad habits & passing on the bad information. you inoculate jars and let them colonize. once colonized its often called spawn. spawning  them is the physical act of mixing your colonized grain(spawn) to your bulk substrate. so on and so on also paul stammets is a hack and his books are loaded with bad information and outdated techniques i assumed thats what books you were showing. the best thing you can do is read thru trusted and proven teks on here. every question you could possibly have to ask has already been asked 1,000 times. the search bar in the upper right is the best tool this site has to offer. you can literally ask it a question like you were talking to someone and it will tell you what you want to know. once learned how to use it properly that is. also when i said shoot it with a shotgun that was obviously just an attempt at adding some color to the fact that the thing is a horrible idea and will give you so many problems you will eventually feel like shooting it with a shotgun😃 to start look up bods and pastys teks and go from there. good luck to ya


--------------------
PANAEOLUS FRUITING MADE SIMPLE



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OfflineRivendell
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Re: My First Grow Journal [Re: jcm4620]
    #26612278 - 04/19/20 07:55 AM (3 years, 9 months ago)

Appreciate the feedback.. Definitely a noob cultivator, but have put some effort into research prior to starting all this.  Here through the 100s of forums and read/reread a bunch of the posted teks.  By no means hating on the community knowledge.  Trying to suck it up like a sponge.  Right now is literally the first experiment and trying to get a setup going that can do trays (vs small shoeboxes) and straw bags (have the lime/straw/container to sterlize on the way from Amazon)  for my oysters, etc.  As for Bods/Pastys.. literally had the up from the beginning and have plans to follow for the agar pour tek and the bucket coir tek.  Can you elaborate some more on the Stamets book issue?  I'd heard a lot of good things and am using it as a manual for the different life cycles/stages to understand more as I grow into this hobby.
Any specifics that I should look out for?  Sorry I missed the playfulness..seems some want to see failure.  I'm ok with failure, because its the only way you learn (as long as one actually learns the lessons from the failures).  Which is why I'm posting a journal vs just doing it on my own and not just lurking.


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Offlinebigmanmcbig
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Re: My First Grow Journal [Re: Rivendell]
    #26612348 - 04/19/20 08:19 AM (3 years, 9 months ago)

Don't let people get you down my man. I think what you have is amazing and you will have a lot of fun like I did messing around and having successes. You're good sir.


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Offlineredhandmat
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Re: My First Grow Journal [Re: bigmanmcbig]
    #26612372 - 04/19/20 08:28 AM (3 years, 9 months ago)

Quote:

bigmanmcbig said:
Don't let people get you down my man. I think what you have is amazing and you will have a lot of fun like I did messing around and having successes. You're good sir.




I (at least) was not tryign to get him down. Im new myself and wasnt sure if I had missed some things. So I asked to see if what he was doing was necessary. He now made it clear that he is just playing with it and trying some things out like his SAB FH mix. And thats cool! This is a hobby, and we do it because we find it fun. So the more fun you have the better :laugh: (just maybe make it clear as to not confuse people)


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OfflineRivendell
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Re: My First Grow Journal [Re: redhandmat]
    #26612397 - 04/19/20 08:37 AM (3 years, 9 months ago)

Nah.. you guys aren't getting me down. I've seen some of the vinegar on the FB groups and know what to expect.  Also just setting expectations with those that aren't fans of my setup.. that this is my very first attempt at mushie cultivation and I'm still in the process of getting things pulled together.  Just a virgin breaking his cherry.  I get that I have a ton to learn and I do appreciate the feedback.

So what should I look out for in the Stamets as outdated knowledge?  I do like the charts, etc they provide for tracking.. as that's the science of it all.  Also learning more about the lifecycle & stages.. troubleshooting section and per species grow info is useful.  (I'm assuming that is whats off?)  Right now I'm a sponge and don't even have the language down right..lol


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Offlineredhandmat
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Re: My First Grow Journal [Re: Rivendell]
    #26612434 - 04/19/20 08:48 AM (3 years, 9 months ago)

Quote:

Rivendell said:
Nah.. you guys aren't getting me down. I've seen some of the vinegar on the FB groups and know what to expect.  Also just setting expectations with those that aren't fans of my setup.. that this is my very first attempt at mushie cultivation and I'm still in the process of getting things pulled together.  Just a virgin breaking his cherry.  I get that I have a ton to learn and I do appreciate the feedback.

So what should I look out for in the Stamets as outdated knowledge?  I do like the charts, etc they provide for tracking.. as that's the science of it all.  Also learning more about the lifecycle & stages.. troubleshooting section and per species grow info is useful.  (I'm assuming that is whats off?)  Right now I'm a sponge and don't even have the language down right..lol




Im an academic (not in mycology but in sociology and business studies) so I respect wanting to follow the science behind it and seeking to optimize parameters (such as temp, humidity, etc) and make efficient use of all resources. But I have learned that when it comes to practical things, such as actually growing plants or mushrooms, you should start by keeping it as simple as possible and getting attention to the basics: so actually growing mushrooms by going print/syringe/tissue to agar plates, cleaning agar plates, then to grain and spawning to simple tecs like this SFF tek . And then growing mushrooms, choosing clones, isolating good strains etc.

Much of this is contingent on not making it harder than it should. As an example that SFF tek I linked above is done so its a do-it-and-forget-it tek that makes efficient use of your time and space. IMO thats especially important for when you want to some day go to commercial as you have said. And once you have a few growths on your belt you can make use of science books to really dial in and optimize even more parameters. But at least then you can judge what it working for you and what is not working. By now we know that a good PC and Flow Hood is something that you can make use of for a long while.


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OfflineRivendell
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Re: My First Grow Journal [Re: redhandmat] * 1
    #26612545 - 04/19/20 09:33 AM (3 years, 9 months ago)

Here are my materials for the next steps I'm planning on jumping into.

PC


My agar and mixing flask


Have a collection of petri dishes
plastic


borosicilate glass - 100mm/60mm


Coir Bucket tek/pellets for bags


Getting some materials for straw bags/cold lime pasteurization that haven't been delivered yet.. Maybe some other things I'm not thinking of.. but as I'm reading the preferred teks of each stage I'm trying to emulate it.


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Offlinebigmanmcbig
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Re: My First Grow Journal [Re: Rivendell] * 2
    #26612827 - 04/19/20 11:55 AM (3 years, 9 months ago)

Sweet stuff my man. go crazy


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Offlineredhandmat
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Re: My First Grow Journal [Re: bigmanmcbig] * 1
    #26612849 - 04/19/20 12:03 PM (3 years, 9 months ago)

looks awesome!


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OnlineLogicaL ChaosM
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26612850 - 04/19/20 12:04 PM (3 years, 9 months ago)

That's some Top-Shelf equipment and supplies u got there! We aint fuckin around! :eek:


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OfflineRivendell
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Re: My First Grow Journal [Re: LogicaL Chaos] * 1
    #26612932 - 04/19/20 12:32 PM (3 years, 9 months ago)

lol.. thanks, not trying to anyways ;-)

If I'm getting into it.. might as well dive in the deep end.


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Re: My First Grow Journal [Re: Rivendell] * 1
    #26612936 - 04/19/20 12:35 PM (3 years, 9 months ago)

Might as well get the good stuff, aimrite?

:popcorn:


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26612942 - 04/19/20 12:38 PM (3 years, 9 months ago)

Have the temp dialed in and stable now at 83.3.  Humidity seems to be an issue keeping up though (although it was at 93% @ the lower temp prior to intro of the heater unit). 


Might have too much water in the lower chamber (although it does seem to be churning out fog and filling the lower resevoir)...

Can see the fogger bubbling and the chamber humid


or the rise in heat might be sapping the humidity, which I noticed as I was getting the temp boosted up.. it was dropping at a corresponding level.  (seen here at 86% earlier on)


I don't see or feel any leaks on the PVC pipes.. hmm..



Also live in the SW US so humidity is an issue inherently in the environment.


Edited by Rivendell (04/19/20 01:00 PM)


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26612944 - 04/19/20 12:39 PM (3 years, 9 months ago)

Exactly.  While I understand some choose the BRF/PF Tek methods to start and learn.. I know I have to get here with some of this equipment/tek anyways.. so why not cannonball in.. make a few hits and misses.. learn.. then eventually churn out canopies.

See you have 56k posts Logical.. if you have thoughts along the way, I'm here to learn, improve & work on perfecting techniques and BE.


Edited by Rivendell (04/19/20 01:02 PM)


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26616635 - 04/21/20 12:10 AM (3 years, 9 months ago)

Day 3 - Not much seen on the existing grain.  Gave it a little shake, but not much else to report there.. so onto the main event.. Today was agar day! 

Finally got around to reading up/vids on YT for proper operation and conditioning of the All American, so I thought I'd give it a test run and make some agar along the way.

First step was to Prep the All American 921 Pressure Canner.  Gave it a nice soap and water bath followed by lubricating up the seal.  Used vaseline per the Kneady Homesteader on YT.. seems she uses AA PC a lot for canning so followed her prep tek





Marked a kabob stick at 2" and 3" for reference markers.


Filled up the PC to approx 2.75" of water


Next up.. mix and cook up some agar.  Used BODs agar tek https://www.shroomery.org/forums/showflat.php/Number/21922023 with a PDA recipe https://www.shroomery.org/forums/showflat.php/Number/4308913. 

- 400 ml water
- 10g agar agar
- 5g potato flakes
- 10ml honey
- 6 drops red dye







Microwaved to boiling point per tek and transferred to PC.. (with rubber flask cap in foil ball to sterilize when used to top in SAB)



Closed the PC up and checked gaps around..




Time to light up the stove!




Finally! Now we wait for the PC to get up to pressure... Happy 4/20/20 everyone!



Pressure is almost there.. Stabilizing at 250/15 psi..



Now we PC for 15 min @ 15 psi


Everything seemed to go ok.. so lets cut off the stove and let her cool to 0 psi



Cracked the PC open and looks like everything survived



Its cooled a little and with the assistance of a hand towel, I take the agar downstairs and place it into the SAB.  Turn on positive pressure fans pushing HEPA filtered air into the SAB and let environment settle (I know there are opinions on this.. I'm going to run with it until I see reason not to on agar) 



Where a new sleeve of plastic petri dishes await.  Also have a scalpel to cut the sleeve and an iso 70% soaked paper towel to wipe everything down.



I wipe down everything with the iso 70% include the petri dishes freshly removed from sleeve and start to pour stacks.  Its my first time so I have to work on the depth consistency.. but I have a bunch of plates prepped



Going to let them sit in there over night and cool/gel.. Have the UV light on so that'll do its magic.. and we'll see what things look like tomorrow!

Phew!


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26620894 - 04/22/20 07:41 PM (3 years, 9 months ago)

Quick update -

Inoculated some MS to agar yesterday.  1 gourmet - the Black Poplar/Pioppino and 2 cubes - Golden Teacher & B+. 

Made sure to get 4 or 5 drops per plate.. probably excessive, but I did see a lot of spores on the plates which made me hopeful that I don't have too high a contam/fail rate.  Used a sterilized (flame) loop to zigzag solution across agar.



Seems to be quite a bit of condensation on the top... is this more of a plastic PP5 vs Borosilicate glass issue?




I'm sure mostly due that I didn't let it cool enough.  I'll know more for the next pour, but something to keep an eye on.  Still a few more gourmets and cubes to put to plate.

Looks like I'll have 1 less plate for the next round of transfers though..


How long does it take for spores to start developing on agar?  I'm seeing some things saying 8 days to 2 weeks, so more curious as this is the first attempt.  Also see a few threads suggesting maybe the sterilized loop wasn't the way to go?

Still plenty to learn.. if you have any suggestions or tips, feel free to drop a note (be nice.. literally first time for all of this).


Edited by Rivendell (04/22/20 07:51 PM)


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26625560 - 04/24/20 07:48 PM (3 years, 9 months ago)

Trying to regulate the environment better, while allowing for better airflow locally to the boxes.. so enter the indoor greenhouse (EZ-UP)



Have it set back a little from wall, we'll see how it regulates the heat... but have removed the painters plastic around the SAB/Fruiting Chamber.


Hopefully being able to pull back the heater will allow the humidity to come back up... but also have a second fogger coming tomorrow to double the HP on the humidity.



Front and rear doors to the greenhouse.. have the back closed up and folded under to keep an air flow seal


Windows on the sides.. we'll see how the air flow goes.. keeping the one window closed to keep heat in better


the exterior basement windows don't have best seals.. and there is a crawl space as well.. so we'll see if the one window is enough.. if not I'll adjust until the environment is stable and regulated better.


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Re: My First Grow Journal [Re: Rivendell]
    #26625590 - 04/24/20 07:58 PM (3 years, 9 months ago)

Very cool set up. But your overthinking the whole thing.

Oh but please do carry on cause now I'm totally sucked in to see how this turns out! It's almost as good as Tiger King.


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OfflineRivendell
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Re: My First Grow Journal [Re: Sockadin]
    #26625601 - 04/24/20 08:03 PM (3 years, 9 months ago)

Its really cold down there.. without the heater, temps are in the low 60s - insert the heater.

The humidity drops as the temp rises, so I'm going to see how adding another fogger effects the system. 

Still dialing in and playing with things.. at least gotta give me some love for the PDA agar for my first attempt ;-)


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Re: My First Grow Journal [Re: Rivendell]
    #26625610 - 04/24/20 08:06 PM (3 years, 9 months ago)

Haha just have fun with it man. I said my thing about how you don't need 1/2 of the shit you bought to grow. But I'm intrigued. Also I'm not like a "right way to be" guy.

So if you want to row your boat in your own direction. Dude it's your boat.

We are here to help if you have any questions. Carry on! This is my new favorite thread btw.


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OfflineRivendell
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Re: My First Grow Journal [Re: Sockadin]
    #26625631 - 04/24/20 08:13 PM (3 years, 9 months ago)

I'm listening and reading.. I hear ya on the SAB/FH angle.. I'll probably get there but like I said, virgin grow attempt.  Breaking my cherry so a lot of what I'm doing is trying to figure things out.

And just wait!  We still have Bucket Tek for Coir.. then mix with spawn (after this colonizes and I drop the transferred agar into new bird seed jars).

And to top it off, looking at the Jiffy Mix casing tek.

Also need to get a liner in the tubs.. trying to read and learn.  Interesting threads here and on FB.  (Shroomery/Agar of Asgard/Several cultivation groups)

So yeah.. having fun and trying to figure this stuff out.. don't even have a concept of how long before I see any mycelium or contam.  We'll see.. logging and will be better 6 months from now I'm sure.


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell] * 1
    #26634600 - 04/28/20 02:58 PM (3 years, 8 months ago)

Starting to see some growth.. since I squirted about 1mL per syringe, need to clean up and start isolating the cultures. 

Pioppino


Golden Teacher


B+


Probably going to T1 transfer this evening.  Thoughts?


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26637432 - 04/29/20 06:47 PM (3 years, 8 months ago)

Have done the T1 Transfers

Pioppino




Golden Teachers





B+




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Offlineredhandmat
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Re: My First Grow Journal [Re: Rivendell]
    #26641506 - 05/01/20 02:57 PM (3 years, 8 months ago)

when you transfer, especially the first transfers, try taking a very small part of the mycelium. Say the equivalent of a rice corn. that way, probabilities to transfer contams is small. Otherwise, its looking good imo! Not sure if all the plates are clean as you are taking pictures from afar. but some of these are looking like myc AFAIK


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OfflineRivendell
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Re: My First Grow Journal [Re: redhandmat]
    #26641678 - 05/01/20 04:22 PM (3 years, 8 months ago)

Yeah, there was an issue with lighting.. as in I didn't have any in the SAB (just the UV light - seen in the blue tinted reflection in pics). However, I just put an LED light bar in there last night.  Should make the pics mush nicer moving forward.

Other big issue is condensation of the petri dishes.  I've noticed that when the petris for T1 were on standby, with the original inoculated cultures still growing, there was no condensation. 

However, when I did my T1 transfer, there was condensation on most all of the plates.  I know that myc does produce heat, but I doubt that's the full reason.

Gonna look for a mason jar to put some hot water in, seal and then put next to the stack (after I iso wipe it that is).

* (and yeah, I know the chunks were big, but being my 1st transfer/use of agar I wanted to hedge my bets.  T2 transfer will have small and better isolated section of solid rhizo growth)


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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26642441 - 05/01/20 11:56 PM (3 years, 8 months ago)

T1 Transfer starting to show some signs of taking off.. a few more days and I'll be spinning up a T2 transfer set (smaller more aggressive growth samples)

Forgive the condensation issues.. still not sure whats up with that, but gave each lid a quick iso wipe to clear the water (have done this a few times now, so far no signs of contam)

Interesting to see the tomentose? growth of the Pioppino





BP



Vs the Rhizomorphic growth
GT





B+



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OfflineRivendell
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Re: My First Grow Journal [Re: Rivendell]
    #26673310 - 05/15/20 06:35 PM (3 years, 8 months ago)

The MS grain is starting to colonize..
BP



GT



Gave those a shake and we'll see where it's at in a few days


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