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magichappens
Stranger

Registered: 04/16/20
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please help .s
#26606019 - 04/16/20 02:51 PM (3 years, 9 months ago) |
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hey shroomery friends, i looking here for a while, fresh new sing up finally. I have trouble about cultivation, i'm wait for your ideas and help. Whatever i did, grain jars didn't progress. After inoculation myc begin to grow in third day generally, but after then stopped, i did different moisture content jars,if too wet myc begin to grow after then stop, if too dry didn't show anything. I finally set ideal MC but still same. Liquid culture looks fine i guess, i can't figure out the problem, i'm stuck, i'm really need help. Here pics.
 Edit: i bought rye from different sources, try different variants : soak 12/24hour, simmer 5/10/20min, no drying/15min drying/30min drying, 1 to 5 ml liquid culture, also spore, pc 1 to 1.5 hour, or steam cooker 2 hour. I should notice steam jars grow faster but anyway they all stop.
Edited by magichappens (04/16/20 02:56 PM)
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Ombisha
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Registered: 02/17/20
Posts: 667
Loc: Tartaria
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okey, so, what did you use to inoculate your jars?
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Mycoactive
Scientist


Registered: 11/20/19
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Last seen: 1 month, 14 days
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Re: please help .s [Re: Ombisha]
#26606085 - 04/16/20 03:36 PM (3 years, 9 months ago) |
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What do the lids look like?
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magichappens
Stranger

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Re: please help .s [Re: Ombisha]
#26606094 - 04/16/20 03:41 PM (3 years, 9 months ago) |
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i use liquid culture, aprox 2-3-4cc for per quart jar, also tried 1.5cc spore, if you ask @Mycoactive i can only find basic lid jars in my location but i'm sure its sterile
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Ombisha
Transmutant



Registered: 02/17/20
Posts: 667
Loc: Tartaria
Last seen: 1 year, 4 months
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you need to modify the lid to allow for air exchange.
Was the liquid culture knocked up with spores? Thats always a shit shoot... you have to get a clean culture first.
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Morbility
Cybernetic Psychonaut


Registered: 12/04/18
Posts: 166
Loc: Ilus IV
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@Ombisha: Pic #3 looks like he used LC since all the myc is starting from the bottom, which is pretty typical.
@Onlinemagichappens: We need a little more info to help you out. A few questions from me:
- How long are you letting the jars colonize before declaring them stalled?
- How old is your LC and are you shaking/swirling it as you inoculate each jar?
- Are you using a still air box to do your work?
LC can be kind of hit-or-miss. I've had very weak LC that I didn't allow to grow long enough and used it anyway. Out of the 10 jars I used it on, 8 didn't do anything.
I've also had LC grow rapidly in some jars and not in others because I was lazy about breaking the myc apart and didn't swirl between jars. I just pour from a media bottle, though, so if OP is using a syringe for his LC this wouldn't really factor in.
LC inoculations can also be veeerrrrrry lazy in some cases for no apparent reason. I've seen jars take over a month to colonize and still produced viable shoeboxes when they were finally ready to spawn.
-------------------- Picture-Perfect Poly Lids Simple & Temporary One-Piece Metal Lids "Life is a great wheel. Sometimes it grinds you down into the mud, and other times it lifts you up into the light." "Are we strapped to this wheel?" "That's a given. The point is, is that most times you get a second chance. You just gotta wait for the wheel."
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magichappens
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Registered: 04/16/20
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Liquid culture is clean, i read before gas exchange not important or maybe i read wrongπ€¦ββοΈ i'll poke jars and fill with polyfill or micropore in sterile environment, i hope work fine. Thanks a lot you save me. i'll update in few days.
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Morbility
Cybernetic Psychonaut


Registered: 12/04/18
Posts: 166
Loc: Ilus IV
Last seen: 1 year, 7 months
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LC jars don't need gas exchange as long as your nutrients are low. Once you inoculate the grain jars it is definitely required. I doubt you will be able to add gas exchange holes to the jars that are colonizing now and have success, since your tape or Poly-Fil needs to be sterilized in the pressure cooker as well. You should be fine in the future though. If you're going to use Poly-Fil, just make sure it's very tight in the hole or you'll have contamination problems. It also helps to trim the Poly-Fil to about 1cm on both sides of the lid after you've poked it through.
-------------------- Picture-Perfect Poly Lids Simple & Temporary One-Piece Metal Lids "Life is a great wheel. Sometimes it grinds you down into the mud, and other times it lifts you up into the light." "Are we strapped to this wheel?" "That's a given. The point is, is that most times you get a second chance. You just gotta wait for the wheel."
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magichappens
Stranger

Registered: 04/16/20
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Last seen: 3 years, 8 months
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heyyoi, rescue operation is succesful, thanks again how i ignore gas exchange i dont know, new jars also began to colonize, with rescued jars i have 27 jar total π i noticed without air exchange in first days colonizing really fast, now new jars exact same variants go slow, any idea why is that? Also thanks again a lot, i cant notice the problem by myself ππ»
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