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OfflineTrippyMomp
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IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG
    #26605577 - 04/16/20 11:02 AM (3 years, 9 months ago)

ok I know I messed up bought a grow kit. its a Midwest growkit with 5 grain spawn bags 2 of them. Inoculated both with 7 ccs of spore culture via syringe. How do you know if a bag needs more water, its been 3 days and no signs of mycelium bags don't seem as wet and steamy as other bags ive seen online, does that just happen once allot of growth has happened from heat. Just nervous my bag is too dry, I used it the day I received the shipment and they claim to send out freshly made bags. Bought this kit to learn about the hobby really would appreciate advice.


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Offlineshevanel
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26605608 - 04/16/20 11:09 AM (3 years, 9 months ago)

A little dry is better than too wet.

Germination of spores can take days to weeks. Just check back on it in week or two.


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OfflineMorbility
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: shevanel]
    #26605676 - 04/16/20 11:43 AM (3 years, 9 months ago)

:whathesaid:

1st rule of Mush Cult: Cultivate your patience.
2nd rule of Mush Cult: Cultivate your patience.

Honestly though, you'd be surprised at how little moisture is needed to make these things grow. I'm still wrapping my head around dialing it in, but if it seems dry there's a good chance it's fine. Make sure you keep your heat in check too--70F to 75F is ideal, 80F is edging up against promoting contamination. Things usually start to take off once you see a little patch of myc beginning, but the waiting can be bitch.


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OfflineTrippyMomp
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: shevanel]
    #26606105 - 04/16/20 03:46 PM (3 years, 9 months ago)

thank you very much really appreciate the advice I figured I'm just being impatient and just don't know what to expect. I put the remaining 6 ccs of liquid culture into a sterile liquid culture jar as an insurance policy. I will keep you guys posted. I'm using Pink Thai Elephant shrooms, the jar will also additionally tell me if I got good spores so I like the thought of that as well.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26606114 - 04/16/20 03:51 PM (3 years, 9 months ago)

Also temperature is set to 75 in my house. Lately its actually been worrying me these past couple nights have dropped into the 50/60s leaving it about 70 degrees in the am this should still be ok though right. Also it sounds like I put a little extra juice in the bags thinking it would help. glad I only did 7 ccs not like 30 which I heard from another post and it being way to soggy. Ill keep you posted guys. Also anything that works as good as the aerosol Lysol literally cant find any these days with this corona shit. I have other "mold cleaning products which ive been avidly wiping and sterilizing. Also as far a temp goes when it gets hotter in the summer I also bought the kit that has thermos and humidity regulation. Hopefully this 110$ will be a good learning experience to say the least.


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OfflineA.k.aM
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26606118 - 04/16/20 03:53 PM (3 years, 9 months ago)

The myc generates heat as it grows which causes condensation.

Lc is tricky especially with spores, if it grows I’d make a brf paste plate to test it on.

Cleaning won’t really do anything, the bag should be sterile.

And damn five pounds of grain for 110?? Maybe I should get in the grow kit game.

I know you know but man you could’ve bought a pressure cooker a case of jars and 20lbs of bird seed for that and ran shoeboxes nonstop for months.


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Edited by A.k.a (04/16/20 03:56 PM)


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OfflineMorbility
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26606155 - 04/16/20 04:27 PM (3 years, 9 months ago)

I agree with A.k.a. It's always good to keep your grow area clean and sanitized, but spraying Lysol, Oust, etc. around only makes the air smell pretty and wastes your cleaning products. The only sterile environment in a grow is inside your jars or your bags.

A temperature dip at night isn't going to hurt anything. It might slow your colonization down a bit, but as long as you aren't freezing your spawn you're fine.


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OfflineTrippyMomp
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility]
    #26606568 - 04/16/20 07:53 PM (3 years, 9 months ago)

yeah i wish i would have done more research, the sellers was reputable and i guess i got so excited i stopped looking into details.. I have a pressure cooker for food that will work in the future I assume. I dont have any Agar plates unfortunately. When I move i plan to have a better area dedicated to it. What do you think are the odds this thing takes off.? along with the spores i injected in the substrate jar of liquid. Shouldn't I see visible activity in the jar before my bags.


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OfflineA.k.aM
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26606582 - 04/16/20 07:59 PM (3 years, 9 months ago)

I bet it’ll go. I did like 20+ spore syringe to grain grows when I started.

Probably 90% of spores I’ve worked with germinated by day 5.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: A.k.a]
    #26606604 - 04/16/20 08:09 PM (3 years, 9 months ago)

What’s this sterile liquid culture jar you shot spores into? Just curious. Did you order a pre-made and sterilized LC?


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Roger Clemency]
    #26606883 - 04/16/20 10:14 PM (3 years, 9 months ago)

yeah premade and sterilized, its a mason jar with a self healing injector port and a filter. maybe that was a wrong move


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26606889 - 04/16/20 10:21 PM (3 years, 9 months ago)

dope this is all really useful I think technically today is the end of day 3. I injected them on Tuesday evening. Its all super interesting for sure. ONE thing im also really worried about is contamination. After the bag is colonized and its time to dump it in the casting and all that with the fancy kit it bought do I need  to trip about cleanliness. I WOULD BE SO MAD AT MY SELF IF I GROW GREEN MOLD LOL


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26606979 - 04/16/20 11:26 PM (3 years, 9 months ago)

The odds are not stacked in your favor putting spores straight to grain. I have done it myself countless times and only mold to show for it. If you want to increase your chances of growing fruits you will want to learn agar. This hobby isn't quick and easy which is why the idea of squirting some spores on grain and growing mushrooms is a false advertisement. Lots of reading, patience, and cleanliness is how you end up being successful in this hobby.

Having said that people do get lucky from time to time but I wouldn't put all your spores in 2 grain bags (pun intended).


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: CocaineBuffet]
    #26607589 - 04/17/20 09:14 AM (3 years, 9 months ago)

It depends on the syringes.

I bought four from a big vendor when I started and did tons of syringe to grain grows and never lost a jar. Sometimes I had a little bacteria but still got a good grow out of all of them.

I also made spore lcs, finally tested two when I got agar and both came up clean which shocked me.


My theory is anywhere that sells PE syringes will probably have clean syringes since they have to use a machine to get the spores that also separates contams.

At least according to what I read. And experienced myself.


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OfflineTrippyMomp
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: A.k.a]
    #26607791 - 04/17/20 10:29 AM (3 years, 9 months ago)

fair enough can only wait and see I guess. hopefully these next couple days the myc takes off. Today the bags were noticeably way more concinnated, trying to not get my hopes up to much but wouldn't that kinda indicate a heat reaction aka the spores becoming alive and breaking down the seed.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26607818 - 04/17/20 10:37 AM (3 years, 9 months ago)

also any recommendations for spores if this fails ill need more obviously. still not seeing any changes in my liquid culture jar that's inoculated as well. Need a good spore site for the U.S. Preferably thanks squad.


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OfflineMorbility
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26608489 - 04/17/20 04:06 PM (3 years, 9 months ago)

Hate to disappoint you, but you're probably just seeing normal condensation in that bag. Noticeable moisture this early in the grow doesn't relate to mycelial activity since there isn't tons of it happening. It can definitely cause a ramp in humidity once things take off and your spawn is colonized, though.

We can't discuss vendors here for the purposes of growing, since spores are intended for microscopy use only. Any of the bigger/older vendors that are advertised here should be a perfectly good source of scientific material in the States. Also keep in mind that you sometimes get what you pay for depending on who you source from. :wink:


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"Life is a great wheel. Sometimes it grinds you down into the mud, and other times it lifts you up into the light."
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility]
    #26612572 - 04/19/20 09:49 AM (3 years, 9 months ago)

Hey Morbidity or anyone else. Im gonna set up pf tek, how big of an issue is it that I bought the taller half pint jars. I didn't see wide mouth ones..if its a huge issue ill go buy the others however if it really isn't that big of a deal then am I  good? Also realized my LC I ordered was not incubating at the right temp. stuck it in with my kombucha heater for fermentation which was at 80 turned it up to 83 and  it seems like the black spore specs are reproducing and conglomerating together


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OfflineMorbility
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp] * 1
    #26613038 - 04/19/20 01:34 PM (3 years, 9 months ago)

I only do tubs and shoeboxes, so I can't really speak to PF tek aside from what I've read. You can use regular sized jars if you want to try it out, but once you're ready to remove the cakes it can be much more difficult than with the wide mouth ones. I think shoeboxes are better for people just starting out, but that's just my opinion. PF tek will definitely work as a learning tool, but to me it seems like a lot of extra work and babysitting needed vs. just setting up a shoebox.

I strongly advise against breaking out a heater/warming mat/etc. At 80F you're pushing the edge of promoting contams, and it isn't going to ramp your colonization time up that much. Ideal temps are 70F-75F or thereabouts. A little above or below is okay, but unless you're trying to grow in a garage without insulation in the Winter, heating your grow up is going to cause more problems than it solves, especially since you're still learning how things should look, etc.


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"Life is a great wheel. Sometimes it grinds you down into the mud, and other times it lifts you up into the light."
"Are we strapped to this wheel?"
"That's a given. The point is, is that most times you get a second chance. You just gotta wait for the wheel."


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Offlinepoisoned
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility]
    #26613105 - 04/19/20 02:25 PM (3 years, 9 months ago)

Tall PF cakes often have issues with compression, which makes the bottoms hard to colonize sometimes.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: poisoned]
    #26613203 - 04/19/20 03:32 PM (3 years, 9 months ago)

Yeah man ditch the heater lc doesn’t need it. They go quick anyways.

I forget what jars these are but I think this is what you’re talking about.



I only made a few but it was fine. I guess occasionally they stall out from something relates to gas exchange. I’ve heard claims if it stalls you can flip it upside down or on the side to make it finish but idk.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: A.k.a]
    #26613487 - 04/19/20 05:49 PM (3 years, 9 months ago)

fair enough I might just order some online I do have two plastic shoe box size totes how do you set those up opposed to pf tek. I just watched the video on it so it makes sense but im up for any suggestions if its as easy as pf tek then im down It seems like I have everything but the perlite which ill prob just order via amazon.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26613505 - 04/19/20 05:59 PM (3 years, 9 months ago)

I don’t like pf tek myself.

Shoeboxes you just mix colonized grain with coir.


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OfflineTrippyMomp
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: A.k.a]
    #26613528 - 04/19/20 06:10 PM (3 years, 9 months ago)

so anything grain related is for a dump and mix type of thing huh ? ive heard you can food process your cakes or cheese grate them to mix them with casing, ever tried that. this is all just super new and fascinating. Can you do agar to pf tek inoculation ? I have some agar stuff on the way also ordered lions main mycelium just for fun too.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26613531 - 04/19/20 06:13 PM (3 years, 9 months ago)

how do I get coir ?


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OfflineMorbility
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26613538 - 04/19/20 06:18 PM (3 years, 9 months ago)

Yup, grains are dump n' mix. Couldn't be simpler. You don't need perlite for shoeboxes. They're just smaller scale tubs, so you'd just be using whatever grain spawn you've decided on and coir to start out.

I've never heard of anyone doing agar to PF, since PF is inoculated with a syringe. You probably could rig up a way to do it, but if you're working with agar it makes PF unnecessary aside from experimentation and playing around.

People have had success grating up their cakes and mixing them with their substrate of choice, but again, if you're going to learn how to do things with agar you're just looking at creating more work for yourself.

Coir can be had on Amazon or as reptile bedding at a pet store. You want the solid bricks of it that need to be rehydrated, but a bag of loose stuff will work too if that's all you can get.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility]
    #26613578 - 04/19/20 06:40 PM (3 years, 9 months ago)

well I guess I just have the stuff to do agar later. I just kept reading that agar is the way to go and sometimes I don't know who to believe but I have more than enough stuff to keep me busy I have the taller 1/2 pint jars now and the shorty's coming from amazon have perlite on the way I figure im to committed  to not try pf tek now. hoping this LC grows more I haven't seen any frothiness yet. the guy said he would get me a new syringe if this one doesn't pan out but im bummed about the grains if they don't  work lol


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OfflineMorbility
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26613855 - 04/19/20 08:46 PM (3 years, 9 months ago)

Your grains could still work out. It's waaaayy too early to be declaring them a lost cause after 3 days. I've had good LC take well over a week to show up in my grain jars after inoculation, but everything you see on here would have you thinking that no recovery from LC after 3 days means it's done for. I've also had spores take 2 weeks to show signs of life on agar. Hell, I had a stalled jar that didn't fully colonize sitting for over two months that I spawned to a shoebox for the hell of it and I just pulled the first harvest today. Genetics, environment, experience, and tons of other things all factor into how fast you see things take off.

It's also really difficult not to helicopter your projects when starting out. I'm still somewhat guilty of it over a year later, but we learn more as experience develops. I've got a triched out tub in quarantine right now that's still giving me tons of side pins to harvest. I just poke my head in every now and then to see how big my fruits are and then go back to being a couch potato locked in my house while I wait for flush #5 to finish out. :grin: And I've got a shoebox fruiting. And I've got jars colonizing and dishes waiting for transfers. Once you get your additional gear in you'll be able to branch into other aspects of the hobby, and that helps cut down on stress and loving your grows to death.

PF tek is totally cool if you went all in. There's a reason people still use it after all this time. It isn't going to get you mad canopies, but you'll definitely learn from it. I'm of the mindset that if you have any interest in doing more than just little stuff or want to explore this as a hobby, agar=mandatory. It opens up tons of avenues and makes things much cleaner and predictable. It's 100% the way to go, and it's not difficult at all. The amount of info here is overwhelming and even intimidating at times, but for a nerd like me it's fun as hell.


--------------------

Picture-Perfect Poly Lids
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"Life is a great wheel. Sometimes it grinds you down into the mud, and other times it lifts you up into the light."
"Are we strapped to this wheel?"
"That's a given. The point is, is that most times you get a second chance. You just gotta wait for the wheel."


Edited by Morbility (04/19/20 08:49 PM)


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Offlinepoisoned
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility]
    #26614332 - 04/20/20 04:45 AM (3 years, 9 months ago)

If you're already doing A2A, you can also do A2G. It's the same procedure, there's just a different substrate below that lid.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: poisoned]
    #26614453 - 04/20/20 06:28 AM (3 years, 9 months ago)

To be clear and thanks for all the help guys. I was naive and have learned more since inoculation. I took spores directly and put them in  two 5 grain bags. I then put the remaining spores into a premade sterilized LC jar that I bought. So I'm not giving up but have come to the conclusion that I would have had a much better chance getting the spores awake first. It's all very interesting. Also my grains are under a water heater It was sitting at 79 which sketched me out my house temp is like 75 all the time. Think I can just keep it at 75 to buffer it from dropping lower than that ? It was either 75 or 79 on the temp control so I dont have allot of middle ground.  Also only been since tuesday since I injected the grain bags. Can you visibly isolate with agar or do I need a microscope as well.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26614456 - 04/20/20 06:30 AM (3 years, 9 months ago)

I'll put a list up of all the supplies I've ordered in the past two days when I get out of bed.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26614459 - 04/20/20 06:35 AM (3 years, 9 months ago)

This is my lc jar hopefully you can see what's growing and tell me if this looks right down bu the magnet little black dots are conglomeating into dots almost together by hair like structures


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26614844 - 04/20/20 09:58 AM (3 years, 9 months ago)

ultimately my goal is to be self sufficient and no longer need to order spores etc. online. Kinda excited to mess with this lions main when it arrives.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26614934 - 04/20/20 10:35 AM (3 years, 9 months ago)

Hey dude I had a quick look through your thread. And it feels to me like you really want to succeed with this. Im a noob myself, but Im starting to see some results. What I can tell you though is that a good way to start is through agar. Its awesome if you can get proper petri dishes and all, but even pp5 like pastywhites easy tek will get you somewhere quicker than shooting syringes you're not sure are clean into grain or pf tek.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: redhandmat]
    #26615385 - 04/20/20 01:25 PM (3 years, 9 months ago)

I really do want to succeed. I tend to obsess over hobbies so I feel like this one will keep me busy. I have 20 petri dishes a dissection lit and agar powder and clean syringes all on the way could totally do agar I'm making my sab box today. Also got an inoculation loop have a nice torch etc. I think everything to do agar but how do you transfer agar to a syringe you make some sort of LC with the clean agar ?


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26615426 - 04/20/20 01:42 PM (3 years, 9 months ago)

Nice! Sounds like you're all set to jump in and really get into things. Since you've got syringes coming, I highly recommend doing the Josex Poke for LC. It takes a little longer to colonize, but it's never once failed me. It works for doing dish-to-dish transfers as well. The tek is a little lengthy to read, but if you follow it exactly as he describes you can't go wrong.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility]
    #26619584 - 04/22/20 09:02 AM (3 years, 9 months ago)

Were approaching the beginning of day 8 since spore inject into my 5 grain bags. Starting to get a lil stanky in my closet might move to a diffent area of my house lol. My LC jar looks like its finally colonizing little floating black specs of the spore attching to hair/string like white stuff floating around. What do I have to lose from reinjecting with the alive  mycelium now ? I know I risk contam but dont want this bag to go to waste what do I have to lose lol. Also when should i consider the first inject a loss and maybe try again ? I was told that can be 20 plus days to germinate. Those grains would be ripe af by then lol based on how they smell now


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26619674 - 04/22/20 09:46 AM (3 years, 9 months ago)

If they smell bad, they're probably bad.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: poisoned]
    #26619768 - 04/22/20 10:21 AM (3 years, 9 months ago)

:whathesaid:

You shouldn't be smelling anything at this point. If you are, I guarantee whatever you're growing isn't mushrooms. Sorry to say dude, but it sounds like this is a no-go.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility]
    #26620013 - 04/22/20 12:58 PM (3 years, 9 months ago)

So its not normal for grains to smell a tiny bit in bags. Just kinda smells like sweaty grains it's not like spoiled yet. But its being incubated in a plastic toat it's been like 73-75 degrees with my house fluctuating at night. I have 2 syringes of gt on the way for microscopy purposes;).


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620015 - 04/22/20 12:59 PM (3 years, 9 months ago)

How would I know if my LC is clean and not contaminated


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620018 - 04/22/20 01:01 PM (3 years, 9 months ago)

What would I have to lose if I pressure cook my used old syringe take some of the LC out and shoot it in there ? I wont have my clean syringes till the end of the month


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620814 - 04/22/20 06:49 PM (3 years, 9 months ago)

I based my response on your description of the smell. Usually you can smell the grains a little through whatever filter is on the jar or bag, but it shouldn't be overpowering. Anything strong or off-putting means bad news.

LC is tough to tell good from bad. That's one of the reasons it's not recommended for people new to the hobby until you get a little more experience. The only way I know of is to look at the LC in the jar--it should be free floating colonies of myc toward the middle (think jellyfish). If it's lazy, staying near the bottom, and not really uniform in shape at first it's probably no good. If you see foam or scum forming on the top, also not good. I give them a sniff test after I use them to inoculate just to be to sure about what I dumped into my jars, but there's no foolproof way to verify.

Quote:

What would I have to lose if I pressure cook my used old syringe take some of the LC out and shoot it in there ?



You risk contaminating the spawn bags as well as the LC that you're trying to cultivate. You can absolutely reuse the syringe in the future, but if you try to reinoculate a questionable grow with questionable LC, I'd estimate you have about a 90% chance of guaranteeing outright failure because you're still learning a lot of this stuff for the first time. Some people get lucky doing stuff like that, but from what I've read it looks like more failures vs. successes with similar attempts.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility]
    #26620864 - 04/22/20 07:21 PM (3 years, 9 months ago)

I think maybe it was just concentrated smell because of the lid being shut. The colonies are starting to float around allot more. I might just run a mini grow op woth the lc to test the lc I have allot of all the ingredients now. Think I'm gonna let my bag sit another week before I toss it


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620911 - 04/22/20 07:49 PM (3 years, 9 months ago)

I think the mini grow is a good idea. If the bags work in the meantime you'll know everything was viable, and if it doesn't end up colonizing you won't be left wondering what would've happened if you hadn't touched it.


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"Life is a great wheel. Sometimes it grinds you down into the mud, and other times it lifts you up into the light."
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620919 - 04/22/20 07:54 PM (3 years, 9 months ago)

Your best bet would be to buy the ingredients to make agar plates (pasty plates or similar no pour methods are easy to start with)

With agar you can check your LC but you can also grow out clean cultures and make LC with it you know will likely be clean.

Agar is the best way, even for beginners, IMO.

https://www.shroomery.org/forums/showflat.php/Number/19208976

https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: sh4d0ws]
    #26621461 - 04/23/20 03:55 AM (3 years, 9 months ago)

What chemicals can I use in place of rubbing alcohol and couod I just buy like a bottle of Smirnoff 100 proof ,? Its that or I buy some shit for like 20$ for 32 ounce alcohol on offer up lol. Want my SAB to be clean and I dont have a ton of isopropyl


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26621463 - 04/23/20 03:57 AM (3 years, 9 months ago)

While iso has a lot of uses in this hobby, it's completely unnecessary to clean your SAB with it. I just wash it out with warm water and put it on the table.


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: poisoned]
    #26621490 - 04/23/20 04:53 AM (3 years, 9 months ago)

Quote:

poisoned said:
While iso has a lot of uses in this hobby, it's completely unnecessary to clean your SAB with it. I just wash it out with warm water and put it on the table.




Here are some quotes from experienced cultivators:
Quote:

Psilosopherr said:
I used to use only iso, and noticed improvements contam wise after switching to soapy water.

frank horrigan has a nice write-up of how to use an SAB in my sig

I still use ISO to sanitize tools/jars that go into said SAB personally. Not sure how necessary this is though..



Quote:

PussyFart said:
I use a real towel in the bottom of my SAB...it is usually soaked in 2-5% bleach solution, or a simple green mix, or just tap water.

I use paper towels soaked in 70% to sanitize everything that goes in.





Im a noob, but I agree with this. I think that soap and water go a long way. You should first and foremost clean the area where you will be working, vacuum, let the dust in the air after vacuuming settle, turn off the AC, and then mop the floor, wipe bench, SAB and everything relevant with water and soap. Go take a shower, change of clothes, brush your teeth etc. Go to the working room (touch as little stuff as possible with your hands). Put a towel sprayed with soap and water (could even add some chlorine maybe) in the bottom of your SAB. If you have iso then you could wipe everything you need as you put it inside the SAB, if not then rely on having clean hands and water and soap to wipe with. I usually go wash my hands thoroughly one last time before I start working inside the SAB.

Now inside the SAB, your hands should never ever hover of any sterilized and open agar plate, grain jar, wte. You should red hot torch any metal equipment you touch agar/spores or wte with (scalpel/inoculation loop/syringe needle). So in practice your hands shouldn't be doing anything but minimizing movements, be slow, deliberate and precise in your hand movements. Always play through the scenario of your actions before you actually do them.


If you do it this way and read well up on sterile tek and watch videos etc you should do fine. I really recommend you like so many others here to forget about LC in the time being, seek instead to develop agar tek. Its sooo much easier to literally SEE the contaminants, and in the long term it lessens the time to get clean culture.

I would go like this: wte medium you have now (lc/spore) take it to agar -> clean the agar through several transfers -----> once a clean agar plate is obtained transfer parts of it to multiple agar plates -> and then transfer some of them to grain jars (WBS/Rye/...wte) -> spawn your grain jars to coir (monotubs/shoeboxes /...wte) --> Bring your mycelium to fruits! and now you can eat/clone/take prints/do LCs...wte you want man!

IMPORTANT note: I read againg through your questions and you are touching on something very very important when you ask "how should I know that my LC is clean?". The answer that I have reached in my limited experience, and hours spent reading in the forums is: you don't. And that's why we use agar to begin with! Never assume that the spores/syringe/LC you get are clean. They most likely are not. So we use agar to see if they are clean, and when they turn out not to be we continue using the agar to clean it up by transfering the clean parts to new agar plates. Once we do that, we test it by fruiting those plates in grain and substrate and see the results.


Edited by redhandmat (04/23/20 05:03 AM)


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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility] * 1
    #26650169 - 05/05/20 09:31 AM (3 years, 8 months ago)

Quote:

Morbility said:
:whathesaid:

1st rule of Mush Cult: Cultivate your patience.
2nd rule of Mush Cult: Cultivate your patience.

Honestly though, you'd be surprised at how little moisture is needed to make these things grow. I'm still wrapping my head around dialing it in, but if it seems dry there's a good chance it's fine. Make sure you keep your heat in check too--70F to 75F is ideal, 80F is edging up against promoting contamination. Things usually start to take off once you see a little patch of myc beginning, but the waiting can be bitch.





this is piggy backing off an old post but this looks promising right ? I did a transfer from a GT plate that had way to much going on in it.  Probably wasn't the correct transfer but I was clean about it and just put the chunk on the new agar.
This is the plate I originally flooded on accident. people said it looked pretty heavy with  bacteria. but there was also myc formation so I took a chunk and transferred it to new media.

Transfer:

I'm super new to this but there's deff been development its grown higher since yesterday. should have no problem growing down onto the new agar right. Since everything's on a microscopic level.


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