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OfflineTrippyMomp
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Registered: 04/16/20
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620015 - 04/22/20 12:59 PM (3 years, 9 months ago)

How would I know if my LC is clean and not contaminated


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OfflineTrippyMomp
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Registered: 04/16/20
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620018 - 04/22/20 01:01 PM (3 years, 9 months ago)

What would I have to lose if I pressure cook my used old syringe take some of the LC out and shoot it in there ? I wont have my clean syringes till the end of the month


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OfflineMorbility
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620814 - 04/22/20 06:49 PM (3 years, 9 months ago)

I based my response on your description of the smell. Usually you can smell the grains a little through whatever filter is on the jar or bag, but it shouldn't be overpowering. Anything strong or off-putting means bad news.

LC is tough to tell good from bad. That's one of the reasons it's not recommended for people new to the hobby until you get a little more experience. The only way I know of is to look at the LC in the jar--it should be free floating colonies of myc toward the middle (think jellyfish). If it's lazy, staying near the bottom, and not really uniform in shape at first it's probably no good. If you see foam or scum forming on the top, also not good. I give them a sniff test after I use them to inoculate just to be to sure about what I dumped into my jars, but there's no foolproof way to verify.

Quote:

What would I have to lose if I pressure cook my used old syringe take some of the LC out and shoot it in there ?



You risk contaminating the spawn bags as well as the LC that you're trying to cultivate. You can absolutely reuse the syringe in the future, but if you try to reinoculate a questionable grow with questionable LC, I'd estimate you have about a 90% chance of guaranteeing outright failure because you're still learning a lot of this stuff for the first time. Some people get lucky doing stuff like that, but from what I've read it looks like more failures vs. successes with similar attempts.


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"Life is a great wheel. Sometimes it grinds you down into the mud, and other times it lifts you up into the light."
"Are we strapped to this wheel?"
"That's a given. The point is, is that most times you get a second chance. You just gotta wait for the wheel."


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OfflineTrippyMomp
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility]
    #26620864 - 04/22/20 07:21 PM (3 years, 9 months ago)

I think maybe it was just concentrated smell because of the lid being shut. The colonies are starting to float around allot more. I might just run a mini grow op woth the lc to test the lc I have allot of all the ingredients now. Think I'm gonna let my bag sit another week before I toss it


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OfflineMorbility
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620911 - 04/22/20 07:49 PM (3 years, 9 months ago)

I think the mini grow is a good idea. If the bags work in the meantime you'll know everything was viable, and if it doesn't end up colonizing you won't be left wondering what would've happened if you hadn't touched it.


--------------------

Picture-Perfect Poly Lids
Simple & Temporary One-Piece Metal Lids

"Life is a great wheel. Sometimes it grinds you down into the mud, and other times it lifts you up into the light."
"Are we strapped to this wheel?"
"That's a given. The point is, is that most times you get a second chance. You just gotta wait for the wheel."


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Invisiblesh4d0ws
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26620919 - 04/22/20 07:54 PM (3 years, 9 months ago)

Your best bet would be to buy the ingredients to make agar plates (pasty plates or similar no pour methods are easy to start with)

With agar you can check your LC but you can also grow out clean cultures and make LC with it you know will likely be clean.

Agar is the best way, even for beginners, IMO.

https://www.shroomery.org/forums/showflat.php/Number/19208976

https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954


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OfflineTrippyMomp
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: sh4d0ws]
    #26621461 - 04/23/20 03:55 AM (3 years, 9 months ago)

What chemicals can I use in place of rubbing alcohol and couod I just buy like a bottle of Smirnoff 100 proof ,? Its that or I buy some shit for like 20$ for 32 ounce alcohol on offer up lol. Want my SAB to be clean and I dont have a ton of isopropyl


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Offlinepoisoned
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: TrippyMomp]
    #26621463 - 04/23/20 03:57 AM (3 years, 9 months ago)

While iso has a lot of uses in this hobby, it's completely unnecessary to clean your SAB with it. I just wash it out with warm water and put it on the table.


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Offlineredhandmat
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: poisoned]
    #26621490 - 04/23/20 04:53 AM (3 years, 9 months ago)

Quote:

poisoned said:
While iso has a lot of uses in this hobby, it's completely unnecessary to clean your SAB with it. I just wash it out with warm water and put it on the table.




Here are some quotes from experienced cultivators:
Quote:

Psilosopherr said:
I used to use only iso, and noticed improvements contam wise after switching to soapy water.

frank horrigan has a nice write-up of how to use an SAB in my sig

I still use ISO to sanitize tools/jars that go into said SAB personally. Not sure how necessary this is though..



Quote:

PussyFart said:
I use a real towel in the bottom of my SAB...it is usually soaked in 2-5% bleach solution, or a simple green mix, or just tap water.

I use paper towels soaked in 70% to sanitize everything that goes in.





Im a noob, but I agree with this. I think that soap and water go a long way. You should first and foremost clean the area where you will be working, vacuum, let the dust in the air after vacuuming settle, turn off the AC, and then mop the floor, wipe bench, SAB and everything relevant with water and soap. Go take a shower, change of clothes, brush your teeth etc. Go to the working room (touch as little stuff as possible with your hands). Put a towel sprayed with soap and water (could even add some chlorine maybe) in the bottom of your SAB. If you have iso then you could wipe everything you need as you put it inside the SAB, if not then rely on having clean hands and water and soap to wipe with. I usually go wash my hands thoroughly one last time before I start working inside the SAB.

Now inside the SAB, your hands should never ever hover of any sterilized and open agar plate, grain jar, wte. You should red hot torch any metal equipment you touch agar/spores or wte with (scalpel/inoculation loop/syringe needle). So in practice your hands shouldn't be doing anything but minimizing movements, be slow, deliberate and precise in your hand movements. Always play through the scenario of your actions before you actually do them.


If you do it this way and read well up on sterile tek and watch videos etc you should do fine. I really recommend you like so many others here to forget about LC in the time being, seek instead to develop agar tek. Its sooo much easier to literally SEE the contaminants, and in the long term it lessens the time to get clean culture.

I would go like this: wte medium you have now (lc/spore) take it to agar -> clean the agar through several transfers -----> once a clean agar plate is obtained transfer parts of it to multiple agar plates -> and then transfer some of them to grain jars (WBS/Rye/...wte) -> spawn your grain jars to coir (monotubs/shoeboxes /...wte) --> Bring your mycelium to fruits! and now you can eat/clone/take prints/do LCs...wte you want man!

IMPORTANT note: I read againg through your questions and you are touching on something very very important when you ask "how should I know that my LC is clean?". The answer that I have reached in my limited experience, and hours spent reading in the forums is: you don't. And that's why we use agar to begin with! Never assume that the spores/syringe/LC you get are clean. They most likely are not. So we use agar to see if they are clean, and when they turn out not to be we continue using the agar to clean it up by transfering the clean parts to new agar plates. Once we do that, we test it by fruiting those plates in grain and substrate and see the results.


Edited by redhandmat (04/23/20 05:03 AM)


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OfflineTrippyMomp
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Re: IM DUMB I KNOW BUT HELP; moisture content in SPAWN BAG [Re: Morbility] * 1
    #26650169 - 05/05/20 09:31 AM (3 years, 8 months ago)

Quote:

Morbility said:
:whathesaid:

1st rule of Mush Cult: Cultivate your patience.
2nd rule of Mush Cult: Cultivate your patience.

Honestly though, you'd be surprised at how little moisture is needed to make these things grow. I'm still wrapping my head around dialing it in, but if it seems dry there's a good chance it's fine. Make sure you keep your heat in check too--70F to 75F is ideal, 80F is edging up against promoting contamination. Things usually start to take off once you see a little patch of myc beginning, but the waiting can be bitch.





this is piggy backing off an old post but this looks promising right ? I did a transfer from a GT plate that had way to much going on in it.  Probably wasn't the correct transfer but I was clean about it and just put the chunk on the new agar.
This is the plate I originally flooded on accident. people said it looked pretty heavy with  bacteria. but there was also myc formation so I took a chunk and transferred it to new media.

Transfer:

I'm super new to this but there's deff been development its grown higher since yesterday. should have no problem growing down onto the new agar right. Since everything's on a microscopic level.


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