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OfflineDidntSeeYouThere
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Question about inoculation
    #26604447 - 04/15/20 11:41 PM (3 years, 9 months ago)

I have some questions as a noob.

I just inoculated my jars, but would it be bad if I used more than 1cc of the syringe to fill one wide mouth jar?

and should I keep the jars covered with foil or uncovered? I don't have microphone tape rn.

also would it be bad to keep them in the still air box for colonization?

much thanks, peace and love 💘


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Offlineshevanel
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Re: Question about inoculation [Re: DidntSeeYouThere]
    #26604458 - 04/15/20 11:47 PM (3 years, 9 months ago)

Are you innoculating from a spore syringe to brf [pint?] jars there champ?



Edited by shevanel (04/15/20 11:48 PM)


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OfflineDidntSeeYouThere
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Re: Question about inoculation [Re: shevanel]
    #26604471 - 04/15/20 11:59 PM (3 years, 9 months ago)

yes that's right, champ


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Offlineshevanel
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Re: Question about inoculation [Re: DidntSeeYouThere]
    #26604478 - 04/16/20 12:04 AM (3 years, 9 months ago)

Youll be fine. I wouldn't sweat it. Unless it was like 10-15-20cc+ then it could increase potential to cause a problem.

What concerns me is the lack of MP tape. So your inoc/injection hole in the lid is exposed/unfiltered?


Edited by shevanel (04/16/20 12:08 AM)


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OfflineSFS96
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Re: Question about inoculation [Re: DidntSeeYouThere]
    #26604479 - 04/16/20 12:05 AM (3 years, 9 months ago)

Less spores is better than more. Take the foil off. And there’s really no reason to colonize in SAB unless u just want too.


--------------------
How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


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OfflineDidntSeeYouThere
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Re: Question about inoculation [Re: shevanel]
    #26604493 - 04/16/20 12:11 AM (3 years, 9 months ago)

yes its uncovered,exposed and how come it increases potential for a problem?


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Offlineshevanel
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Re: Question about inoculation [Re: DidntSeeYouThere]
    #26604500 - 04/16/20 12:16 AM (3 years, 9 months ago)

Because without a filter the dirty air can bring unwanted microscopic assassins into your jar.


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OfflineSFS96
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Re: Question about inoculation [Re: shevanel]
    #26604507 - 04/16/20 12:22 AM (3 years, 9 months ago)

what kind of jar setup are u using? Iv never used mp tape on any jars but I don’t know what kind of lid setup u have


--------------------
How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


Edited by SFS96 (04/16/20 12:23 AM)


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Offlineshevanel
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Re: Question about inoculation [Re: SFS96]
    #26604510 - 04/16/20 12:25 AM (3 years, 9 months ago)

I assume hes drilled a hole into a lid and inoculated through it like that straight up, no micropore tape to cover the hole afterwards.

If you're implying that isnt required then thats news to me.. however i don't use pftek or brf but i understand sterile technique and practice.


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OfflineSFS96
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Re: Question about inoculation [Re: shevanel]
    #26604516 - 04/16/20 12:29 AM (3 years, 9 months ago)

Yeah it’s fucked if he drilled a hole with no filter but he didn’t specify what his setup was or if it was PF Tek or grain jars.


--------------------
How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


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Offlineshevanel
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Re: Question about inoculation [Re: SFS96]
    #26604524 - 04/16/20 12:33 AM (3 years, 9 months ago)

He confirmed what he was using when i asked [in post 2 & 3] so i just inferred he probably did drill a hole when he mentioned not having any mp tape.


Edited by shevanel (04/16/20 12:36 AM)


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OfflineSFS96
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Re: Question about inoculation [Re: shevanel]
    #26604528 - 04/16/20 12:37 AM (3 years, 9 months ago)

Missed that somehow lol if it’s brf jars there’s no need for mp tape because u use vermiculite at the top of the jars as a filter. Is think mp tape would probably slow down colonization a little bit since there’s already a verm Filter.


--------------------
How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


Edited by SFS96 (04/16/20 12:38 AM)


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Offlineshevanel
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Re: Question about inoculation [Re: SFS96]
    #26604530 - 04/16/20 12:38 AM (3 years, 9 months ago)

Ah, thats neat[about verm layer]. I totally forgot about that. Well, we shall see...lets hope he used it then.


Edited by shevanel (04/16/20 12:48 AM)


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OfflineDidntSeeYouThere
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Re: Question about inoculation [Re: shevanel]
    #26604577 - 04/16/20 01:08 AM (3 years, 9 months ago)

yes I used a verm layer at the top and with brf pf tek jars with regular lid with holes, no mp tape


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Offlineshevanel
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Re: Question about inoculation [Re: DidntSeeYouThere]
    #26604603 - 04/16/20 01:33 AM (3 years, 9 months ago)

Youre golden.


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