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Invisiblemushhead
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From agar to grain - My first agar log
    #26604440 - 04/15/20 11:39 PM (3 years, 9 months ago)

So I recently started some agar plates with B+ Spore and they've been colonizing for about 5 days, I've had some good growth and decided to transfer some myc into a rye jar. I cleaned out my STB and got my jar, tools, and dish inside. I cut out a piece of myc (with some spores on it as well) and dropped it into the jar, I shook the chunk of agar into the side of the jar.

Now given everything works out well, given my cleanliness was enough, would this kind of inoculation succeed? I've only been working with agar for a few weeks now.

Furthermore if this does work, I'll keep this thread updated with pics, maybe I'll try a g2g again.


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Edited by mushhead (04/18/20 07:30 PM)


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Offlineshevanel
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Re: From agar to grain? [Re: mushhead] * 1
    #26604463 - 04/15/20 11:50 PM (3 years, 9 months ago)

Most people would have made a xfer from the MS plate to a new agar plate[and again later]in order to clean up the growth.

What you did [can] work but you havnt minimized the risk of contams by having inoculate thats assured clean.


Edited by shevanel (04/15/20 11:52 PM)


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OfflineSFS96
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Re: From agar to grain? [Re: mushhead]
    #26604467 - 04/15/20 11:52 PM (3 years, 9 months ago)

That’s how it’s done but you should really take multiple transfers to other plates before you go to grain. You might as well just put spores strait to a grain doing it the way you did. The point of agar is to transfer until your sure you have a clean culture. You don’t know if your culture is clean now because you used your germination plate to go to grain.


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Invisiblemushhead
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Re: From agar to grain? [Re: shevanel]
    #26604469 - 04/15/20 11:55 PM (3 years, 9 months ago)

Quote:

shevanel said:
Most people would have made a xfer from the MS plate to a new agar plate in order to clean up the growth.




Yeah I actually have other plates that I transferred to, but there was just a good amount left on the original plate so I thought why not? You haven't really answered my question, but one can assume the answer from your response that its a safe practice - tried and true? Perhaps, I have yet to experience it.


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Offlineshevanel
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Re: From agar to grain? [Re: mushhead]
    #26604473 - 04/15/20 11:59 PM (3 years, 9 months ago)

Using the germination plate to inoculate grain isnt considered a safe practice by any stretch.  Like the other person said. Its almost like using the spore syringe straight to grain. [Which can work] but youre using agar in your practice so thats what youre trying to avoid doing.


Edited by shevanel (04/16/20 12:01 AM)


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Invisiblemushhead
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Re: From agar to grain? [Re: shevanel]
    #26604482 - 04/16/20 12:06 AM (3 years, 9 months ago)

I agree with you, and I know the basic agar transfer process which I am following. I am not without an imagination so while doing transfers I decided to attempt to knock up a jar, did I do correctly? What I mean is when I have a few transfers and some beautiful plates is this the way you knock up your jars?


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OfflineSFS96
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Re: From agar to grain? [Re: mushhead]
    #26604486 - 04/16/20 12:09 AM (3 years, 9 months ago)

Watch the last video here and you will know if u did it right.Easy af cult videos


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Re: From agar to grain? [Re: mushhead]
    #26604491 - 04/16/20 12:11 AM (3 years, 9 months ago)

That's one way. You got it.

I like to drop the wedges [1 plate per master for future G2G] right on top..no shake, no movement..when the myc recovers and later jumps onto the grain and gets a nice golf ball sized thats when i give it some help and shake it up.


Edited by shevanel (04/16/20 12:13 AM)


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Invisiblemushhead
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Re: From agar to grain? [Re: SFS96]
    #26604494 - 04/16/20 12:13 AM (3 years, 9 months ago)

Quote:

SFS96 said:
Watch the last video here and you will know if u did it right.Easy af cult videos




I really appreciate it! I did it almost just as himself there so I'm sure i'll have some good results. I'll post em here why not?


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Re: From agar to grain? [Re: mushhead]
    #26604495 - 04/16/20 12:13 AM (3 years, 9 months ago)

Please do. Everyone loves pics and talking about them.


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Invisiblemushhead
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Re: From agar to grain? [Re: shevanel]
    #26604496 - 04/16/20 12:15 AM (3 years, 9 months ago)

Quote:

shevanel said:
That's one way. You got it.

I like to drop the wedges [1 plate per master for future G2G] right on top..no shake, no movement..when the myc recovers and later jumps onto the grain and gets a nice golf ball sized thats when i give it some help and shake it up.




Alright, hopefully my little shimmying it into the grain wasn't the best idea, but hey we learn.


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Re: From agar to grain? [Re: mushhead]
    #26604505 - 04/16/20 12:20 AM (3 years, 9 months ago)

At least you got a little bit of practice :thumbup: maybe u will get some mushrooms too:mushroom2:


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Re: From agar to grain? [Re: mushhead]
    #26604506 - 04/16/20 12:20 AM (3 years, 9 months ago)

Either way works. Its in there. I dont fuss with it because id rather not: A. Get the wedge stuck to the glass and. B. knock the myc off the wedge so that it recovers as a nice piece not a bunch of teeny tiny pieces if that makes sense.


Edited by shevanel (04/16/20 12:22 AM)


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Offlinepoisoned
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Re: From agar to grain? [Re: shevanel]
    #26604796 - 04/16/20 04:21 AM (3 years, 9 months ago)

Nothing wrong with dropping that plate to grain. Sure, it might contam, but you might also get shrooms quicker. And you get a practice run.

Do some risky attempts if you have a general plan on how to improve on those techniques.


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Edited by poisoned (04/16/20 04:22 AM)


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Invisiblemushhead
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Re: From agar to grain? [Re: poisoned]
    #26605875 - 04/16/20 01:32 PM (3 years, 9 months ago)

Quote:

poisoned said:
Nothing wrong with dropping that plate to grain. Sure, it might contam, but you might also get shrooms quicker. And you get a practice run.

Do some risky attempts if you have a general plan on how to improve on those techniques.




I appreciate the advice truly!



Some amazing growth here, this is the first plate I transferred to, the others are still recovering.

EDIT

Only hours later. I also made up some more plates for the next transfers.


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Edited by mushhead (04/16/20 05:45 PM)


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Invisiblemushhead
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Re: From agar to grain? [Re: mushhead]
    #26607835 - 04/17/20 10:42 AM (3 years, 9 months ago)


Plate looks good, however I did spot some mold growing on the edges as you can tell. I was wondering if I should just grab another cutting after some myc growth or cut off the contams since they haven't sporulated yet.


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Invisiblemushhead
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Re: From agar to grain? [Re: mushhead]
    #26610425 - 04/18/20 12:27 PM (3 years, 9 months ago)



So I decided to just chop off the budding mold before it sporulated and had good results, no other croppings of the pesky hyphae have surfaced as of yet. My other transfers are doing quite well, notice the missing numbers of the ones that didn't quite do so well heh. I'm attempting further transfers today from other ms plates of B+. I'm having a lot of trouble procuring iso alcohol, I can't even get 70% anywhere let alone 91%, this has lead me to the only options available: Lysol, peroxide, hand sanitizer... anyone have any other suggestions that I can attempt to procure?

EDIT: Got my question answered elsewhere: Everclear 190 proof watered down....3 parts Everclear to 1 part water should get you around 70%. Also I ended up just buying 99.9% iso off ebay for an easier solution.


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Edited by mushhead (04/18/20 07:31 PM)


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Invisiblemushhead
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Re: From agar to grain? [Re: mushhead]
    #26611891 - 04/19/20 01:33 AM (3 years, 9 months ago)


Got round two agar plates made up, decided to add a little color to these guys, green is better then basic MEA yellow >~>


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Invisiblemushhead
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Re: From agar to grain? [Re: mushhead]
    #26612916 - 04/19/20 12:27 PM (3 years, 9 months ago)


This morning I took two transfers from this beauty and dropped them onto new agar - However I have some very promising growth on my first attempts!


Now at the start of this thread I asked about proper practice when transferring your agar into jars and I thought i'd check up on that to see how it was doing and I am tickled by the results.



No contamination in sight, wonderful looking myc growth; sure its a MS germination with no transfers at all, but I have high hopes for this jar.


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Edited by mushhead (04/19/20 02:43 PM)


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InvisibleD3_Myc
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Re: From agar to grain? [Re: mushhead]
    #26615454 - 04/20/20 01:56 PM (3 years, 9 months ago)

Looking good! Off to a great start.


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: D3_Myc]
    #26615496 - 04/20/20 02:10 PM (3 years, 9 months ago)



Plate update time! So I recently a mate of mine sent me some prints (You know who you are :mushroom2: ) and I decided to get some of the spores started. There were some mold spores on the tinfoil unfortunately so I had to transfer only a few days after the spores germinated, however they are looking so beautiful white on green. My other B+ plates are really coming along well.


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead] * 1
    #26620725 - 04/22/20 05:58 PM (3 years, 9 months ago)


Some Very good growth over the last few days.

My attempts at isolating myc types(fluffy/rhizomorphic) has been successful.


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InvisibleD3_Myc
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26620793 - 04/22/20 06:37 PM (3 years, 9 months ago)

That plate on the right sexy as hell


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: D3_Myc]
    #26620874 - 04/22/20 07:30 PM (3 years, 9 months ago)

Quote:

D3monic said:
That plate on the right sexy as hell



It's going to be the main plate I take transfers from for when I spawn this to grain. Honestly it was absolute pure luck not uncommon for a leprechaun such as I but that was only my fifth transfer from my first MS plate.


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Edited by mushhead (04/22/20 08:43 PM)


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Offlineroarkell
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Re: From agar to grain - My first agar log [Re: mushhead] * 1
    #26621656 - 04/23/20 07:03 AM (3 years, 9 months ago)

I noticed with all the varieties I've ran that a lower nutrient ratio encouraged more rhizo growth... I've been running 4g LME, 8g Agar and 388g water. True 1% solution.

These 2 were part of a test group I did with varying nutrient ratios.



It's kinda hard to see because they're fully grown but the lower nute (1.25% in this case) was obviously more rhizo then the second (1.5%)

EDIT: I'm currently running this test group out and have pics and details in the Ecuadorian and gulf coast grow log in my journal if you wanna check it out!


Edited by roarkell (04/23/20 07:19 AM)


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: roarkell]
    #26624795 - 04/24/20 01:37 PM (3 years, 9 months ago)


Just wanted to drop an update of the jars I dropped some MS germination plates onto.
They're looking fantastic! I couldn't be more tickled pink.


Here is some beautiful growth, I am allowing the Gulf Coast cubensis to grow out a little more before I take transfers.


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Edited by mushhead (04/24/20 02:54 PM)


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26627280 - 04/25/20 01:58 PM (3 years, 8 months ago)


These are all my active plates rn. I can spot mold in my gulf coast #3 plate, can you? I have already made the transfers and will observe. I have disposed of the contaminated plate after taking transfers as well. I noticed blue bruising on one of my Golden Teacher plates, the myc grew from below pushing the wedge onto the top of the dish, I'll have to observe this for just a bit longer.


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Edited by mushhead (04/25/20 02:07 PM)


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InvisibleD3_Myc
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26627395 - 04/25/20 02:47 PM (3 years, 8 months ago)

This RW looks like it had some mold too





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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: D3_Myc] * 1
    #26627404 - 04/25/20 02:54 PM (3 years, 8 months ago)

OH right ye I forgot to mention that one. Rusty White cubensis, I took transfers of the myc furthest away from that mold, I made over 30 transfers today haha!


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26636570 - 04/29/20 11:05 AM (3 years, 8 months ago)


That B+ plate is going to throw pins soon hopefully and I can get some good clones :shocked:


Going back to the jars I did at the start of this thread - They're doing so freakin' well I couldn't be happier. I have had ZERO contamination with my jars and after I had some good plates with some beautiful growth I made more than just the couple I had.


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InvisibleD3_Myc
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26636640 - 04/29/20 11:37 AM (3 years, 8 months ago)

Looking good!


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: D3_Myc] * 1
    #26653374 - 05/06/20 05:31 PM (3 years, 8 months ago)


It's spawning time :0


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InvisibleD3_Myc
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26653504 - 05/06/20 06:28 PM (3 years, 8 months ago)

:sexeh:


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: D3_Myc] * 1
    #26653534 - 05/06/20 06:38 PM (3 years, 8 months ago)

My feelings exactly demonic, I'm doing a 2qt tub which is the smallest tub I have, and then I'm doing a 5qt tub. each will colonize fully with a casing at the start of coir/verm. Once colonized I plan to place a very thin layer of casing to stimulate pins and keep the substrate from drying out.

Edit:
I changed my mind actually, I am going to use all 7qts in a slightly larger tub I have just acquired.


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Edited by mushhead (05/06/20 08:41 PM)


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26676753 - 05/17/20 11:49 AM (3 years, 8 months ago)

Alright so it's been 10 days since I spawned those 7 qts into
1 brick Coir, 1.5qts Verm, 2 Table spoons gypsum
this is a modified tub with holes first covered with one layer of micropore tape and then polyfil once I deemed it time.
FAE has been introduced slowly and the tub is kept at a steady 73f (or 23c)
I have not misted as it does not need it and wont need it till first fruits begin to mature.

As you can see hyphal knots are beginning to form and I expect fruit within the next week.


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Edited by mushhead (05/17/20 12:04 PM)


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InvisibleD3_Myc
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26676786 - 05/17/20 12:15 PM (3 years, 8 months ago)

Pins very soon!


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: D3_Myc]
    #26676787 - 05/17/20 12:18 PM (3 years, 8 months ago)

:aweyeah:


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Re: From agar to grain - My first agar log [Re: mushhead] * 1
    #26676889 - 05/17/20 01:20 PM (3 years, 8 months ago)

Following.
:scarybeat:


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: X-Ray Cat]
    #26677492 - 05/17/20 06:58 PM (3 years, 8 months ago)

:datass:
:aweyeah:
Since I dropped my B+ plates into grain already, followed by my Gulf Coast plates, I decided to start some APE and Rustywhytes.
The APE i started with LC however I did not take pics of the jar or process during that time :crazy: oh well.


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26681674 - 05/19/20 06:54 PM (3 years, 8 months ago)

Two days later and I have some fine pins showing up!
And some funky blob :rolleyes: Looks like B+ mutating again.

Overview of the entire tub

So far everything is looking fantastic, other than the unknown blob.
Any ideas guys?

EDIT: Got my question answered elsewhere, this is more than likely a genetic mutation and I have nothing to worry over.


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Edited by mushhead (05/19/20 08:49 PM)


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26683010 - 05/20/20 10:25 AM (3 years, 8 months ago)


Some great growth already, though I am seeing more of the blobbing.
I have it under a 12/12 6500 cfl, perhaps I need more FAE and the blobbing will cease?


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26683971 - 05/20/20 06:22 PM (3 years, 8 months ago)


B+ still blobbing away I guess :crazy:
I've increased FAE with no real change, however since the increase I am observing blobs become fruits.
If this was an inevitability or not intrigues me.


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Offlineroarkell
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26684242 - 05/20/20 08:24 PM (3 years, 8 months ago)

Could you get a close up of these blobs you speak of? Those look like pins to me...


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: roarkell]
    #26684258 - 05/20/20 08:30 PM (3 years, 8 months ago)


Here's some of the most prominent ones.
I know they're pins but they're blobbing up.


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OfflineX-Ray Cat
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26684419 - 05/20/20 10:09 PM (3 years, 8 months ago)

You have a few mutants but you're good homie.


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Re: From agar to grain - My first agar log [Re: X-Ray Cat]
    #26684446 - 05/20/20 10:31 PM (3 years, 8 months ago)

Quote:

X-Ray Cat said:
You have a few mutants but you're good homie.



OH OH I know they're mutants and I know I'm fine
But this happens every time I grow this specific B+
Other B+ I have don't do this but these genetics are just wild.


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26688045 - 05/22/20 12:09 PM (3 years, 8 months ago)


Myc finally flushing faster, throwing up some really blobbed caps.
Overall as expected I am beginning to see split caps.
This happened my earlier B+ Grow

So I expect to see just around the same amount of it this cycle.
I wonder if anyone can shed some light onto why this occurs?
I already understand that its more likely to be mutation than environment.


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Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


Edited by mushhead (05/22/20 12:33 PM)


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26690734 - 05/23/20 04:07 PM (3 years, 8 months ago)


And we're about to be at full flush, I expect this to go for another two days before the flush has fully grown in.

Final first flush dry weight 60.04g


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Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


Edited by mushhead (05/25/20 01:04 AM)


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26694066 - 05/25/20 01:56 AM (3 years, 8 months ago)

I'll keep this thread updated with subsequent flushes.
Otherwise thanks for dropping in!


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Re: From agar to grain - My first agar log [Re: mushhead]
    #26707194 - 05/30/20 01:50 PM (3 years, 7 months ago)


Second flush a day or so away from full flush. I expect this one to be a nice canopy.


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26708429 - 05/31/20 03:52 AM (3 years, 7 months ago)


Almost a full canopy!


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26709401 - 05/31/20 03:33 PM (3 years, 7 months ago)

Dried weight 86.56g for the second flush.


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26726077 - 06/07/20 09:41 AM (3 years, 7 months ago)


Here is the Third flush from this tub - Looking ok but Sadly possible contam may have me cutting this run short two flushes.


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26727722 - 06/07/20 10:22 PM (3 years, 7 months ago)

Dimethyltryptamine

On your couch
your mind reached out
into the universe.

In this glimpse
your mind did skip
a lifetime in reverse.

The music lulls
A tune of souls
but you only caught a verse.

You climb the schemes
Of cosmic seams
And in them was immersed.

A lifetime seen
In clockwork dreams
All the places you'll traverse.

You'll be dropped
Back in yourself
Now understood your curse.

In the end
Time still ebbs
And with it we converse.

By: mushhead


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Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


Edited by mushhead (06/07/20 11:12 PM)


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InvisibleD3_Myc
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Re: From agar to grain - My first agar log [Re: mushhead] * 1
    #26728057 - 06/08/20 04:20 AM (3 years, 7 months ago)

Nice haul mush!


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OfflineGastronomicus
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Re: From agar to grain - My first agar log [Re: mushhead] * 1
    #26728200 - 06/08/20 06:18 AM (3 years, 7 months ago)

This is a great log


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: Gastronomicus]
    #26729470 - 06/08/20 05:33 PM (3 years, 7 months ago)

Quote:

D3monic said:
Nice haul mush!



Quote:

Gastronomicus said:
This is a great log



Thank you!
I will keep things updated and there are spore prints available.
I still not sure if its a contam or not so we'll see what gives.
:shrug:


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Invisiblemushhead
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Re: From agar to grain - My first agar log [Re: mushhead]
    #26744967 - 06/14/20 09:09 PM (3 years, 7 months ago)

Hey guys this log is concluded!
Following observation for the past 5 days I have determined that tub to be contaminated.
I have rid myself of it today with a sad sigh.
Thanks for tuning in!


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